| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 813,910 | A→G | 100% | C60C (TGT→TGC) | prsA ← | phosphoribosylpyrophosphate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 813,910 | 0 | A | G | 99.4% | 172.7 / ‑6.6 | 46 | C60C (TGT→TGC) | prsA | phosphoribosylpyrophosphate synthase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (0/45); minor base T (0/1); total (0/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAAT > minE/813848‑813933 | gAAGCTCGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCGCAAg < 1:682979/67‑1 (MQ=255)gAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCGCAAg < 1:1949412/67‑1 (MQ=255)gAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCGCAAg < 1:657152/67‑1 (MQ=255)gAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCGCAAg < 1:591787/67‑1 (MQ=255)gAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCGCAAg < 1:514266/67‑1 (MQ=255)gAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCGCAAg < 1:498454/67‑1 (MQ=255)gAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCGCAAg < 1:462458/67‑1 (MQ=255)gAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCGCAAg < 1:364234/67‑1 (MQ=255)gAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCGCAAg < 1:806731/67‑1 (MQ=255)gAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCGCAAg < 1:2085171/67‑1 (MQ=255)gAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCGCAAg < 1:1934003/67‑1 (MQ=255)gAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCGCAAg < 1:1876943/67‑1 (MQ=255)gAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCGCAAg < 1:1790200/67‑1 (MQ=255)gAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCGCAAg < 1:1021379/67‑1 (MQ=255)gAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCGCAAg < 1:1745216/67‑1 (MQ=255)gAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCGCAAg < 1:971444/67‑1 (MQ=255)gAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCGCAAg < 1:884991/67‑1 (MQ=255)gAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCGCAAg < 1:1473774/67‑1 (MQ=255)gAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCGCAAg < 1:1733796/67‑1 (MQ=255)gAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCGCAAg < 1:893519/67‑1 (MQ=255) aaGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCGCAAg < 1:880308/66‑1 (MQ=255) aaGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCGCAAg < 1:150486/66‑1 (MQ=255) aaGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCGCAAg < 1:762882/66‑1 (MQ=255) cAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCGCAAg < 1:406285/48‑1 (MQ=255) aCTAATTCCATCAGGTTGTCGTTAGTAGGGGCTCAAg < 1:954085/37‑1 (MQ=255) tGTCGTTAGTAGTGGCGCAAGTGGACTGGATGATGAAAat < 1:1746590/40‑1 (MQ=38) tGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGTAAat < 1:1406/40‑1 (MQ=255) tGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAat < 1:759238/40‑1 (MQ=255) tGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAat < 1:698451/40‑1 (MQ=255) tGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAat < 1:821294/40‑1 (MQ=255) tGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAat < 1:687855/40‑1 (MQ=255) tGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAat < 1:992384/40‑1 (MQ=255) tGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAat < 1:666163/40‑1 (MQ=255) tGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAat < 1:603367/40‑1 (MQ=255) tGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAat < 1:472326/40‑1 (MQ=255) tGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAat < 1:367788/40‑1 (MQ=255) tGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAat < 1:352121/40‑1 (MQ=255) tGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAat < 1:2157316/40‑1 (MQ=255) tGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAat < 1:1867768/40‑1 (MQ=255) tGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAat < 1:173819/40‑1 (MQ=255) tGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAat < 1:1610701/40‑1 (MQ=255) tGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAat < 1:1582985/40‑1 (MQ=255) tGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAat < 1:1277859/40‑1 (MQ=255) tGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAat < 1:1163012/40‑1 (MQ=255) tGTCGGTAGTAGGGGCGCAAGTGGACTGGATGATGAAAat < 1:536871/40‑1 (MQ=38) gTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAat < 1:360351/39‑1 (MQ=255) | GAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAAT > minE/813848‑813933 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |