| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 880,753 | G→A | 100% | intergenic (+72/‑122) | yciM → / → pyrF | conserved hypothetical protein/orotidine‑5'‑phosphate decarboxylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 880,753 | 0 | G | A | 100.0% | 78.8 / NA | 22 | intergenic (+72/‑122) | yciM/pyrF | conserved hypothetical protein/orotidine‑5'‑phosphate decarboxylase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (1/21); total (1/21) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AACATACTAATTATTAATGTTCCATTGTGCTCCGGCAACGACGGCGCAGAAAAAGCCTGCCAGGGGAGAAATCGCAACTGTTAATTT > minE/880710‑880796 | aacaTACTAATTATTAATGTTCCATTGTGCTCCGGCAACGACGACGCAGAAAAAGCCTGCCAgggg > 1:2091864/1‑66 (MQ=255) ccATTGTGCTCCGGCAACGACTACGCAGAAAAAGCCTGCCAGGGGAGAAATCGCAACTGTTAAttt < 1:734404/66‑1 (MQ=255) ccATTGTGCTCCGGCAACGACGACGCAGAAAAAGCCTGCCAGGGGAGAAATCGCAACTGTTAAttt < 1:2107026/66‑1 (MQ=255) ccATTGTGCTCCGGCAACGACGACGCAGAAAAAGCCTGCCAGGGGAGAAATCGCAACTGTTAAttt < 1:966254/66‑1 (MQ=255) ccATTGTGCTCCGGCAACGACGACGCAGAAAAAGCCTGCCAGGGGAGAAATCGCAACTGTTAAttt < 1:887257/66‑1 (MQ=255) ccATTGTGCTCCGGCAACGACGACGCAGAAAAAGCCTGCCAGGGGAGAAATCGCAACTGTTAAttt < 1:761971/66‑1 (MQ=255) ccATTGTGCTCCGGCAACGACGACGCAGAAAAAGCCTGCCAGGGGAGAAATCGCAACTGTTAAttt < 1:708230/66‑1 (MQ=255) ccATTGTGCTCCGGCAACGACGACGCAGAAAAAGCCTGCCAGGGGAGAAATCGCAACTGTTAAttt < 1:623430/66‑1 (MQ=255) ccATTGTGCTCCGGCAACGACGACGCAGAAAAAGCCTGCCAGGGGAGAAATCGCAACTGTTAAttt < 1:595677/66‑1 (MQ=255) ccATTGTGCTCCGGCAACGACGACGCAGAAAAAGCCTGCCAGGGGAGAAATCGCAACTGTTAAttt < 1:560864/66‑1 (MQ=255) ccATTGTGCTCCGGCAACGACGACGCAGAAAAAGCCTGCCAGGGGAGAAATCGCAACTGTTAAttt < 1:327579/66‑1 (MQ=255) ccATTGTGCTCCGGCAACGACGACGCAGAAAAAGCCTGCCAGGGGAGAAATCGCAACTGTTAAttt < 1:292758/66‑1 (MQ=255) ccATTGTGCTCCGGCAACGACGACGCAGAAAAAGCCTGCCAGGGGAGAAATCGCAACTGTTAAttt < 1:1089562/66‑1 (MQ=255) ccATTGTGCTCCGGCAACGACGACGCAGAAAAAGCCTGCCAGGGGAGAAATCGCAACTGTTAAttt < 1:2033700/66‑1 (MQ=255) ccATTGTGCTCCGGCAACGACGACGCAGAAAAAGCCTGCCAGGGGAGAAATCGCAACTGTTAAttt < 1:1652336/66‑1 (MQ=255) ccATTGTGCTCCGGCAACGACGACGCAGAAAAAGCCTGCCAGGGGAGAAATCGCAACTGTTAAttt < 1:1566402/66‑1 (MQ=255) ccATTGTGCTCCGGCAACGACGACGCAGAAAAAGCCTGCCAGGGGAGAAATCGCAACTGTTAAttt < 1:1383121/66‑1 (MQ=255) ccATTGTGCTCCGGCAACGACGACGCAGAAAAAGCCTGCCAGGGGAGAAATCGCAACTGTTAAttt < 1:127632/66‑1 (MQ=255) cATTGTGCTCCGGCAACGACGACGCAGAAAAAGCCTGCCAGGGGAGAAATCGCAACTGTTAAttt < 1:1169778/65‑1 (MQ=255) gtgCTCCGGCAACGACGACGCAGAAAAAGCCTGCCAGGGGAGAAATc < 1:2079595/47‑1 (MQ=255) gtgCTCCGGCAACGACGACGCAGAAAAAGCCTGCCAGGGGAGAAATc < 1:1687817/47‑1 (MQ=255) gtgCTCCGGCAACGACGACGCAGAAAAAGCCTGCCAGGGGAGAAATc < 1:1415619/47‑1 (MQ=255) | AACATACTAATTATTAATGTTCCATTGTGCTCCGGCAACGACGGCGCAGAAAAAGCCTGCCAGGGGAGAAATCGCAACTGTTAATTT > minE/880710‑880796 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |