Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	1,027,446	A→G	100%	N134S (AAC→AGC)	malX →	fused maltose and glucose‑specific PTS enzyme IIBC components

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,027,446	0	A	G	91.7%	32.8 / ‑3.1	12	N134S (AAC→AGC)	malX	fused maltose and glucose‑specific PTS enzyme IIBC components
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (0/11);  total (1/11)
Fisher's exact test for biased strand distribution p-value = 8.33e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATCCAGTCGATCGATA  >  minE/1027409‑1027481                                      |                                    cTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAGCATCCag                              <  1:11187/45‑1 (MQ=255) cTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAGCATCCag                              <  1:1244855/45‑1 (MQ=255) cTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAGCATCCag                              <  1:1410990/45‑1 (MQ=255) cTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAGCATCCag                              <  1:1804252/45‑1 (MQ=255) cTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAGCATCCag                              <  1:1894014/45‑1 (MQ=255) cTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAGCATCCag                              <  1:2074351/45‑1 (MQ=255) cTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAGCATCCag                              <  1:268057/45‑1 (MQ=255) cTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAGCATCCag                              <  1:357258/45‑1 (MQ=255) cTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAGCATCCag                              <  1:428581/45‑1 (MQ=255) cTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAGCATCCag                              <  1:675019/45‑1 (MQ=255)  tGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAGCATCCag                              <  1:538006/44‑1 (MQ=255)         cACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATCCAGTCGATCGATa  >  1:1842400/1‑65 (MQ=255)                                      |                                    CTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATCCAGTCGATCGATA  >  minE/1027409‑1027481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑