Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,027,446 A→G 100% N134S (AAC→AGC)  malX → fused maltose and glucose‑specific PTS enzyme IIBC components

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,027,4460AG91.7% 32.8 / ‑3.1 12N134S (AAC→AGC) malXfused maltose and glucose‑specific PTS enzyme IIBC components
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (0/11);  total (1/11)
Fisher's exact test for biased strand distribution p-value = 8.33e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATCCAGTCGATCGATA  >  minE/1027409‑1027481
                                     |                                   
cTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAGCATCCag                              <  1:11187/45‑1 (MQ=255)
cTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAGCATCCag                              <  1:1244855/45‑1 (MQ=255)
cTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAGCATCCag                              <  1:1410990/45‑1 (MQ=255)
cTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAGCATCCag                              <  1:1804252/45‑1 (MQ=255)
cTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAGCATCCag                              <  1:1894014/45‑1 (MQ=255)
cTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAGCATCCag                              <  1:2074351/45‑1 (MQ=255)
cTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAGCATCCag                              <  1:268057/45‑1 (MQ=255)
cTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAGCATCCag                              <  1:357258/45‑1 (MQ=255)
cTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAGCATCCag                              <  1:428581/45‑1 (MQ=255)
cTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAGCATCCag                              <  1:675019/45‑1 (MQ=255)
 tGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAGCATCCag                              <  1:538006/44‑1 (MQ=255)
        cACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATCCAGTCGATCGATa  >  1:1842400/1‑65 (MQ=255)
                                     |                                   
CTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATCCAGTCGATCGATA  >  minE/1027409‑1027481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: