Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,111,527 G→A 100% C287Y (TGT→TAT)  ydiD → short chain acyl‑CoA synthetase, anaerobic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,111,5270GA100.0% 275.3 / NA 74C287Y (TGT→TAT) ydiDshort chain acyl‑CoA synthetase, anaerobic
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (0/74);  total (0/74)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTGTCTCGCGCTGCTTGAGCAGCAGCGTTGCACCTGTATGCTCGGCGCAACGCCGTTTG  >  minE/1111482‑1111583
                                             |                                                        
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTTagca                                        <  1:31229/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca                                        <  1:396183/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca                                        <  1:1863089/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca                                        <  1:1868881/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca                                        <  1:1879787/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca                                        <  1:1922353/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca                                        <  1:1924862/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca                                        <  1:2004253/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca                                        <  1:2047295/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca                                        <  1:2057719/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca                                        <  1:2083148/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca                                        <  1:2114738/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca                                        <  1:216167/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca                                        <  1:264622/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca                                        <  1:315513/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca                                        <  1:348691/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca                                        <  1:1018169/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca                                        <  1:403148/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca                                        <  1:408906/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca                                        <  1:431766/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca                                        <  1:452696/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca                                        <  1:457625/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca                                        <  1:46729/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca                                        <  1:472396/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca                                        <  1:557053/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca                                        <  1:619956/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca                                        <  1:701390/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca                                        <  1:73532/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca                                        <  1:739301/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca                                        <  1:749166/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca                                        <  1:873604/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca                                        <  1:925147/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca                                        <  1:1096228/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca                                        <  1:1247611/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca                                        <  1:1308357/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca                                        <  1:1314987/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca                                        <  1:1323618/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca                                        <  1:132742/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca                                        <  1:1365642/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca                                        <  1:1383481/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca                                        <  1:140104/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca                                        <  1:1220288/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca                                        <  1:1152348/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca                                        <  1:1140463/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca                                        <  1:1411631/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca                                        <  1:1445662/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca                                        <  1:1093331/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca                                        <  1:1075945/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca                                        <  1:1550037/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca                                        <  1:105290/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca                                        <  1:1041879/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca                                        <  1:1592644/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca                                        <  1:159734/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca                                        <  1:1616152/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca                                        <  1:1007409/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca                                        <  1:1766468/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca                                        <  1:1775458/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTCagca                                        <  1:4130/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTAGagca                                        <  1:944171/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCATGATGCGTATCTCGCGCTGCTTGagca                                        <  1:15048/64‑1 (MQ=255)
ttGGCGCTCGCAGCGTGTTGGTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca                                        <  1:1403959/64‑1 (MQ=255)
 tGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTTCTTGagca                                        <  1:1580631/63‑1 (MQ=255)
 tGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca                                        <  1:271020/63‑1 (MQ=255)
 tGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca                                        <  1:2100637/63‑1 (MQ=255)
 tGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca                                        <  1:722918/63‑1 (MQ=255)
 tGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca                                        <  1:203185/63‑1 (MQ=255)
 tGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca                                        <  1:1024035/63‑1 (MQ=255)
 tGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca                                        <  1:89012/63‑1 (MQ=255)
             cGTGTTGTTAGATATTTTCACTCCTGATGGGTATCTGGCGCTGCTTGagca                                        <  1:1224066/51‑1 (MQ=255)
                  tgttAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca                                        <  1:2139193/46‑1 (MQ=255)
                   gttAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca                                        <  1:1630011/45‑1 (MQ=255)
                      aGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca                                        <  1:298546/42‑1 (MQ=255)
                                   ccTGATGCGTATCTCGCGCTGCTTGAGCAGCAGCGTTGCACCTGTATGCTCGGCGCAACGCCGTTTg  <  1:84477/67‑1 (MQ=255)
                                   ccTGATGCGTATCTCGCGCTGCTTGAGCAGCAGCGTTGCACCTGTATGCTCGGCGCAACGCCGTTTg  <  1:177205/67‑1 (MQ=255)
                                             |                                                        
TTGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTGTCTCGCGCTGCTTGAGCAGCAGCGTTGCACCTGTATGCTCGGCGCAACGCCGTTTG  >  minE/1111482‑1111583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: