| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,111,527 | G→A | 100% | C287Y (TGT→TAT) | ydiD → | short chain acyl‑CoA synthetase, anaerobic |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,111,527 | 0 | G | A | 100.0% | 275.3 / NA | 74 | C287Y (TGT→TAT) | ydiD | short chain acyl‑CoA synthetase, anaerobic |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (0/74); total (0/74) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TTGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTGTCTCGCGCTGCTTGAGCAGCAGCGTTGCACCTGTATGCTCGGCGCAACGCCGTTTG > minE/1111482‑1111583 | ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTTagca < 1:31229/64‑1 (MQ=255)ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:396183/64‑1 (MQ=255)ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:1863089/64‑1 (MQ=255)ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:1868881/64‑1 (MQ=255)ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:1879787/64‑1 (MQ=255)ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:1922353/64‑1 (MQ=255)ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:1924862/64‑1 (MQ=255)ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:2004253/64‑1 (MQ=255)ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:2047295/64‑1 (MQ=255)ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:2057719/64‑1 (MQ=255)ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:2083148/64‑1 (MQ=255)ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:2114738/64‑1 (MQ=255)ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:216167/64‑1 (MQ=255)ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:264622/64‑1 (MQ=255)ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:315513/64‑1 (MQ=255)ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:348691/64‑1 (MQ=255)ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:1018169/64‑1 (MQ=255)ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:403148/64‑1 (MQ=255)ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:408906/64‑1 (MQ=255)ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:431766/64‑1 (MQ=255)ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:452696/64‑1 (MQ=255)ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:457625/64‑1 (MQ=255)ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:46729/64‑1 (MQ=255)ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:472396/64‑1 (MQ=255)ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:557053/64‑1 (MQ=255)ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:619956/64‑1 (MQ=255)ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:701390/64‑1 (MQ=255)ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:73532/64‑1 (MQ=255)ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:739301/64‑1 (MQ=255)ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:749166/64‑1 (MQ=255)ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:873604/64‑1 (MQ=255)ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:925147/64‑1 (MQ=255)ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:1096228/64‑1 (MQ=255)ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:1247611/64‑1 (MQ=255)ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:1308357/64‑1 (MQ=255)ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:1314987/64‑1 (MQ=255)ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:1323618/64‑1 (MQ=255)ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:132742/64‑1 (MQ=255)ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:1365642/64‑1 (MQ=255)ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:1383481/64‑1 (MQ=255)ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:140104/64‑1 (MQ=255)ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:1220288/64‑1 (MQ=255)ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:1152348/64‑1 (MQ=255)ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:1140463/64‑1 (MQ=255)ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:1411631/64‑1 (MQ=255)ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:1445662/64‑1 (MQ=255)ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:1093331/64‑1 (MQ=255)ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:1075945/64‑1 (MQ=255)ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:1550037/64‑1 (MQ=255)ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:105290/64‑1 (MQ=255)ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:1041879/64‑1 (MQ=255)ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:1592644/64‑1 (MQ=255)ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:159734/64‑1 (MQ=255)ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:1616152/64‑1 (MQ=255)ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:1007409/64‑1 (MQ=255)ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:1766468/64‑1 (MQ=255)ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:1775458/64‑1 (MQ=255)ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTCagca < 1:4130/64‑1 (MQ=255)ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTAGagca < 1:944171/64‑1 (MQ=255)ttGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCATGATGCGTATCTCGCGCTGCTTGagca < 1:15048/64‑1 (MQ=255)ttGGCGCTCGCAGCGTGTTGGTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:1403959/64‑1 (MQ=255) tGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTTCTTGagca < 1:1580631/63‑1 (MQ=255) tGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:271020/63‑1 (MQ=255) tGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:2100637/63‑1 (MQ=255) tGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:722918/63‑1 (MQ=255) tGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:203185/63‑1 (MQ=255) tGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:1024035/63‑1 (MQ=255) tGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:89012/63‑1 (MQ=255) cGTGTTGTTAGATATTTTCACTCCTGATGGGTATCTGGCGCTGCTTGagca < 1:1224066/51‑1 (MQ=255) tgttAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:2139193/46‑1 (MQ=255) gttAGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:1630011/45‑1 (MQ=255) aGATATTTTCACTCCTGATGCGTATCTCGCGCTGCTTGagca < 1:298546/42‑1 (MQ=255) ccTGATGCGTATCTCGCGCTGCTTGAGCAGCAGCGTTGCACCTGTATGCTCGGCGCAACGCCGTTTg < 1:84477/67‑1 (MQ=255) ccTGATGCGTATCTCGCGCTGCTTGAGCAGCAGCGTTGCACCTGTATGCTCGGCGCAACGCCGTTTg < 1:177205/67‑1 (MQ=255) | TTGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTGTCTCGCGCTGCTTGAGCAGCAGCGTTGCACCTGTATGCTCGGCGCAACGCCGTTTG > minE/1111482‑1111583 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |