| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,142,011 | T→C | 100% | F152L (TTC→CTC) | katE → | hydroperoxidase HPII(III) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,142,011 | 0 | T | C | 91.3% | 68.9 / ‑0.5 | 23 | F152L (TTC→CTC) | katE | hydroperoxidase HPII(III) |
| Reads supporting (aligned to +/- strand): ref base T (2/0); new base C (21/0); total (23/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GCCGCTCACGGTTATTTCCAGCCATATAAAAGCTTAAGCGATATTACCAAAGCGGATTTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGTACGTTTCTCTACCGTTC > minE/1141954‑1142065 | gCCGCTCACGGTTATTTCCAGCCATATAAAAGCTTAAGCGATATTACCAAAGCGGAtt > 1:2024351/1‑58 (MQ=255)gCCGCTCACGGTTATTTCCAGCCATATAAAAGCTTAAGCGATATTACCAAAGCGGATTTCct > 1:896552/1‑62 (MQ=255)gCCGCTCACGGTTATTTCCAGCCATATAAAAGCTTAAGCGATATTACCAAAGCGGATTTCct > 1:1755048/1‑62 (MQ=255) aGCTTAAGCGATATTACCAAAGCGGATCTCCTCTCAGATCCGAACAAAATCACCCCAg > 1:1741054/1‑58 (MQ=255) aGCTTAAGCGATATTACCAAAGCGGATCTCCTCTCAGATCCGAACAAAATCACCCCAg > 1:447396/1‑58 (MQ=255) aCCAAAGCGGATCTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGTACGTTTCTCTACCGTTc > 1:2034884/1‑67 (MQ=255) aCCAAAGCGGATCTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGTACGTTTCTCTACCGTTc > 1:1338529/1‑67 (MQ=255) aCCAAAGCGGATCTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGTACGTTTCTCTACCGTTc > 1:844668/1‑67 (MQ=255) aCCAAAGCGGATCTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGTACGTTTCTCTACCGTTc > 1:783673/1‑67 (MQ=255) aCCAAAGCGGATCTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGTACGTTTCTCTACCGTTc > 1:648803/1‑67 (MQ=255) aCCAAAGCGGATCTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGTACGTTTCTCTACCGTTc > 1:571524/1‑67 (MQ=255) aCCAAAGCGGATCTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGTACGTTTCTCTACCGTTc > 1:564508/1‑67 (MQ=255) aCCAAAGCGGATCTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGTACGTTTCTCTACCGTTc > 1:452238/1‑67 (MQ=255) aCCAAAGCGGATCTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGTACGTTTCTCTACCGTTc > 1:276170/1‑67 (MQ=255) aCCAAAGCGGATCTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGTACGTTTCTCTACCGTTc > 1:222430/1‑67 (MQ=255) aCCAAAGCGGATCTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGTACGTTTCTCTACCGTTc > 1:1457398/1‑67 (MQ=255) aCCAAAGCGGATCTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGTACGTTTCTCTACCGTTc > 1:139520/1‑67 (MQ=255) aCCAAAGCGGATCTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGTACGTTTCTCTACCGTTc > 1:1942361/1‑67 (MQ=255) aCCAAAGCGGATCTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGTACGTTTCTCTACCGTTc > 1:1936858/1‑67 (MQ=255) aCCAAAGCGGATCTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGTACGTTTCTCTACCGTTc > 1:1886146/1‑67 (MQ=255) aCCAAAGCGGATCTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGTACGTTTCTCTACCGTTc > 1:1444443/1‑67 (MQ=255) aCCAAAGCGGATCTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGTACGTTTCTCTACCGTTc > 1:1572881/1‑67 (MQ=255) aCCAAAGCGGATCTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGTACGTTTCTCTACCGTTc > 1:1565749/1‑67 (MQ=255) aCCAAAGCGGATCTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGTACGTTTCTCTACCGTTc > 1:1563368/1‑67 (MQ=255) | GCCGCTCACGGTTATTTCCAGCCATATAAAAGCTTAAGCGATATTACCAAAGCGGATTTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGTACGTTTCTCTACCGTTC > minE/1141954‑1142065 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |