Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,198,527 G→C 100% L41V (CTG→GTG)  yoaH ← conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,198,5270GC100.0% 247.6 / NA 65L41V (CTG→GTG) yoaHconserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (65/0);  total (65/0)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TGTGTTACTCGTCTTCATCCTCAAAACGCGCCACGATTCGCTCGCCGGAGTGGTTGGCACGCAGCTC  >  minE/1198464‑1198530
                                                               |   
tgtgtTACTCGTCTTCATCCTCAAAACGCGCCACGATTCGCTCGCCGGAGTGGTTGGCACGCACcta  >  1:532118/1‑66 (MQ=255)
tgtgtTACTCGTCTTCATCCTCAAAACGCGCCACGATTCGCTCGCCGGAGTGGTTGGCACGCACcta  >  1:2004791/1‑66 (MQ=255)
tgtgtTACTCGTCTTCATCCTCAAAACGCGCCACGATTCGCTCGCCGGAGTGGTTGGCACGCACcta  >  1:205732/1‑66 (MQ=255)
tgtgtTACTCGTCTTCATCCTCAAAACGCGCCACGATTCGCTCGCCGGAGTGGTTGGCACGCACcta  >  1:2064008/1‑66 (MQ=255)
tgtgtTACTCGTCTTCATCCTCAAAACGCGCCACGATTCGCTCGCCGGAGTGGTTGGCACGCACcta  >  1:2105129/1‑66 (MQ=255)
tgtgtTACTCGTCTTCATCCTCAAAACGCGCCACGATTCGCTCGCCGGAGTGGTTGGCACGCACcta  >  1:2106668/1‑66 (MQ=255)
tgtgtTACTCGTCTTCATCCTCAAAACGCGCCACGATTCGCTCGCCGGAGTGGTTGGCACGCACcta  >  1:2109126/1‑66 (MQ=255)
tgtgtTACTCGTCTTCATCCTCAAAACGCGCCACGATTCGCTCGCCGGAGTGGTTGGCACGCACcta  >  1:211709/1‑66 (MQ=255)
tgtgtTACTCGTCTTCATCCTCAAAACGCGCCACGATTCGCTCGCCGGAGTGGTTGGCACGCACcta  >  1:22209/1‑66 (MQ=255)
tgtgtTACTCGTCTTCATCCTCAAAACGCGCCACGATTCGCTCGCCGGAGTGGTTGGCACGCACcta  >  1:236399/1‑66 (MQ=255)
tgtgtTACTCGTCTTCATCCTCAAAACGCGCCACGATTCGCTCGCCGGAGTGGTTGGCACGCACcta  >  1:283561/1‑66 (MQ=255)
tgtgtTACTCGTCTTCATCCTCAAAACGCGCCACGATTCGCTCGCCGGAGTGGTTGGCACGCACcta  >  1:303480/1‑66 (MQ=255)
tgtgtTACTCGTCTTCATCCTCAAAACGCGCCACGATTCGCTCGCCGGAGTGGTTGGCACGCACcta  >  1:307563/1‑66 (MQ=255)
tgtgtTACTCGTCTTCATCCTCAAAACGCGCCACGATTCGCTCGCCGGAGTGGTTGGCACGCACcta  >  1:321909/1‑66 (MQ=255)
tgtgtTACTCGTCTTCATCCTCAAAACGCGCCACGATTCGCTCGCCGGAGTGGTTGGCACGCACcta  >  1:358288/1‑66 (MQ=255)
tgtgtTACTCGTCTTCATCCTCAAAACGCGCCACGATTCGCTCGCCGGAGTGGTTGGCACGCACcta  >  1:446858/1‑66 (MQ=255)
tgtgtTACTCGTCTTCATCCTCAAAACGCGCCACGATTCGCTCGCCGGAGTGGTTGGCACGCACcta  >  1:1968976/1‑66 (MQ=255)
tgtgtTACTCGTCTTCATCCTCAAAACGCGCCACGATTCGCTCGCCGGAGTGGTTGGCACGCACcta  >  1:543376/1‑66 (MQ=255)
tgtgtTACTCGTCTTCATCCTCAAAACGCGCCACGATTCGCTCGCCGGAGTGGTTGGCACGCACcta  >  1:632586/1‑66 (MQ=255)
tgtgtTACTCGTCTTCATCCTCAAAACGCGCCACGATTCGCTCGCCGGAGTGGTTGGCACGCACcta  >  1:651446/1‑66 (MQ=255)
tgtgtTACTCGTCTTCATCCTCAAAACGCGCCACGATTCGCTCGCCGGAGTGGTTGGCACGCACcta  >  1:655516/1‑66 (MQ=255)
tgtgtTACTCGTCTTCATCCTCAAAACGCGCCACGATTCGCTCGCCGGAGTGGTTGGCACGCACcta  >  1:686908/1‑66 (MQ=255)
tgtgtTACTCGTCTTCATCCTCAAAACGCGCCACGATTCGCTCGCCGGAGTGGTTGGCACGCACcta  >  1:708834/1‑66 (MQ=255)
tgtgtTACTCGTCTTCATCCTCAAAACGCGCCACGATTCGCTCGCCGGAGTGGTTGGCACGCACcta  >  1:847676/1‑66 (MQ=255)
tgtgtTACTCGTCTTCATCCTCAAAACGCGCCACGATTCGCTCGCCGGAGTGGTTGGCACGCACcta  >  1:86457/1‑66 (MQ=255)
tgtgtTACTCGTCTTCATCCTCAAAACGCGCCACGATTCGCTCGCCGGAGTGGTTGGCACGCACcta  >  1:891179/1‑66 (MQ=255)
tgtgtTACTCGTCTTCATCCTCAAAACGCGCCACGATTCGCTCGCCGGAGTGGTTGGCACGCACcta  >  1:891981/1‑66 (MQ=255)
tgtgtTACTCGTCTTCATCCTCAAAACGCGCCACGATTCGCTCGCCGGAGTGGTTGGCACGCACcta  >  1:908033/1‑66 (MQ=255)
tgtgtTACTCGTCTTCATCCTCAAAACGCGCCACGATTCGCTCGCCGGAGTGGTTGGCACGCACcta  >  1:910537/1‑66 (MQ=255)
tgtgtTACTCGTCTTCATCCTCAAAACGCGCCACGATTCGCTCGCCGGAGTGGTTGGCACGCACcta  >  1:946201/1‑66 (MQ=255)
tgtgtTACTCGTCTTCATCCTCAAAACGCGCCACGATTCGCTCGCCGGAGTGGTTGGCACGCACcta  >  1:981291/1‑66 (MQ=255)
tgtgtTACTCGTCTTCATCCTCAAAACGCGCCACGATTCGCTCGCCGGAGTGGTTGGCACGCACcta  >  1:982745/1‑66 (MQ=255)
tgtgtTACTCGTCTTCATCCTCAAAACGCGCCACGATTCGCTCGCCGGAGTGGTTGGCACGCACcta  >  1:1607082/1‑66 (MQ=255)
tgtgtTACTCGTCTTCATCCTCAAAACGCGCCACGATTCGCTCGCCGGAGTGGTTGGCACGCACcta  >  1:1064216/1‑66 (MQ=255)
tgtgtTACTCGTCTTCATCCTCAAAACGCGCCACGATTCGCTCGCCGGAGTGGTTGGCACGCACcta  >  1:1104121/1‑66 (MQ=255)
tgtgtTACTCGTCTTCATCCTCAAAACGCGCCACGATTCGCTCGCCGGAGTGGTTGGCACGCACcta  >  1:1191364/1‑66 (MQ=255)
tgtgtTACTCGTCTTCATCCTCAAAACGCGCCACGATTCGCTCGCCGGAGTGGTTGGCACGCACcta  >  1:1217822/1‑66 (MQ=255)
tgtgtTACTCGTCTTCATCCTCAAAACGCGCCACGATTCGCTCGCCGGAGTGGTTGGCACGCACcta  >  1:1231581/1‑66 (MQ=255)
tgtgtTACTCGTCTTCATCCTCAAAACGCGCCACGATTCGCTCGCCGGAGTGGTTGGCACGCACcta  >  1:1238639/1‑66 (MQ=255)
tgtgtTACTCGTCTTCATCCTCAAAACGCGCCACGATTCGCTCGCCGGAGTGGTTGGCACGCACcta  >  1:1245050/1‑66 (MQ=255)
tgtgtTACTCGTCTTCATCCTCAAAACGCGCCACGATTCGCTCGCCGGAGTGGTTGGCACGCACcta  >  1:1354829/1‑66 (MQ=255)
tgtgtTACTCGTCTTCATCCTCAAAACGCGCCACGATTCGCTCGCCGGAGTGGTTGGCACGCACcta  >  1:144667/1‑66 (MQ=255)
tgtgtTACTCGTCTTCATCCTCAAAACGCGCCACGATTCGCTCGCCGGAGTGGTTGGCACGCACcta  >  1:1480145/1‑66 (MQ=255)
tgtgtTACTCGTCTTCATCCTCAAAACGCGCCACGATTCGCTCGCCGGAGTGGTTGGCACGCACcta  >  1:1526926/1‑66 (MQ=255)
tgtgtTACTCGTCTTCATCCTCAAAACGCGCCACGATTCGCTCGCCGGAGTGGTTGGCACGCACcta  >  1:15273/1‑66 (MQ=255)
tgtgtTACTCGTCTTCATCCTCAAAACGCGCCACGATTCGCTCGCCGGAGTGGTTGGCACGCACcta  >  1:1565546/1‑66 (MQ=255)
tgtgtTACTCGTCTTCATCCTCAAAACGCGCCACGATTCGCTCGCCGGAGTGGTTGGCACGCACcta  >  1:1605124/1‑66 (MQ=255)
tgtgtTACTCGTCTTCATCCTCAAAACGCGCCACGATTCGCTCGCCGGAGTGGTTGGCACGCACcta  >  1:1060224/1‑66 (MQ=255)
tgtgtTACTCGTCTTCATCCTCAAAACGCGCCACGATTCGCTCGCCGGAGTGGTTGGCACGCACcta  >  1:1790011/1‑66 (MQ=255)
tgtgtTACTCGTCTTCATCCTCAAAACGCGCCACGATTCGCTCGCCGGAGTGGTTGGCACGCACcta  >  1:192677/1‑66 (MQ=255)
tgtgtTACTCGTCTTCATCCTCAAAACGCGCCACGATTCGCTCGCCGGAGTGGTTGGCACGCACcta  >  1:18665/1‑66 (MQ=255)
tgtgtTACTCGTCTTCATCCTCAAAACGCGCCACGATTCGCTCGCCGGAGTGGTTGGCACGCACcta  >  1:1827932/1‑66 (MQ=255)
tgtgtTACTCGTCTTCATCCTCAAAACGCGCCACGATTCGCTCGCCGGAGTGGTTGGCACGCACcta  >  1:1821793/1‑66 (MQ=255)
tgtgtTACTCGTCTTCATCCTCAAAACGCGCCACGATTCGCTCGCCGGAGTGGTTGGCACGCACcta  >  1:1817526/1‑66 (MQ=255)
tgtgtTACTCGTCTTCATCCTCAAAACGCGCCACGATTCGCTCGCCGGAGTGGTTGGCACGCACcta  >  1:1798633/1‑66 (MQ=255)
tgtgtTACTCGTCTTCATCCTCAAAACGCGCCACGATTCGCTCGCCGGAGTGGTTGGCACGCACcta  >  1:1650251/1‑66 (MQ=255)
tgtgtTACTCGTCTTCATCCTCAAAACGCGCCACGATTCGCTCGCCGGAGTGGTTGGCACGCACcta  >  1:1762561/1‑66 (MQ=255)
tgtgtTACTCGTCTTCATCCTCAAAACGCGCCACGATTCGCTCGCCGGAGTGGTTGGCACGCACcta  >  1:1729719/1‑66 (MQ=255)
tgtgtTACTCGTCTTCATCCTCAAAACGCGCCACGATTCGCTCGCCGGAGTGGTTGGCACGCACcta  >  1:1705581/1‑66 (MQ=255)
tgtgtTACTCGTCTTCATCCTCAAAACGCGCCACGATTCGCTCGCCGGAGTGGTTGGCACGCACcta  >  1:1678625/1‑66 (MQ=255)
tgtgtTACTCGTCTTCATCCTCAAAACGCGCCACGATTCGCTCGCCGGAGTGGTTGGCACGCACcta  >  1:1668203/1‑66 (MQ=255)
tgtgtTACTCGTCTTCATCCTCAAAACGCGCCACGATTCGCTCGCCGGAGTGGTTGGCACGCACct   >  1:1266071/1‑66 (MQ=255)
tgtgtTACTCGTCTTCATCCTCAAAACGCGCCACGATTCGCTCGCCGGAGTGGTTGGCACGCACct   >  1:1750969/1‑66 (MQ=255)
tgtgtTACTCGTCTTCATCCTCAAAACGCGCCACGATACGCTCGCCGGAGTGGTTGGCACGCACcta  >  1:1422735/1‑66 (MQ=255)
tgtgcTACTCGTCTTCATCCTCAAAACGCGCCACGATTCGCTCGCCGGAGTGGTTGGCACGCACcta  >  1:2087397/1‑66 (MQ=255)
                                                               |   
TGTGTTACTCGTCTTCATCCTCAAAACGCGCCACGATTCGCTCGCCGGAGTGGTTGGCACGCAGCTC  >  minE/1198464‑1198530

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑