Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,475,665 A→T 100% T244S (ACC→TCC)  nrdB → ribonucleoside diphosphate reductase 1, beta subunit, ferritin‑like

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,475,6650AT100.0% 112.2 / NA 32T244S (ACC→TCC) nrdBribonucleoside diphosphate reductase 1, beta subunit, ferritin‑like
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (26/6);  total (26/6)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ACGCCAAAATTATTCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGA  >  minE/1475618‑1475708
                                               |                                           
aCGCCAAAATTATTCGCCTTATTGCCCGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCATATGc                           >  1:531299/1‑66 (MQ=255)
aCGCCAAAATTATTCGCCTGATTGCCCGCGCCGAAGCCCTGCACCTGTCCGGCACCCAGCATATGc                           >  1:1693078/1‑66 (MQ=255)
aCGCCAAAATTATTCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCATATg                            >  1:92636/1‑65 (MQ=255)
aCGCCAAAATTATTCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCATATg                            >  1:60454/1‑65 (MQ=255)
aCGCCAAAATTATTCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCATATGc                           >  1:343073/1‑66 (MQ=255)
aCGCCAAAATTATTCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCATATGc                           >  1:981235/1‑66 (MQ=255)
aCGCCAAAATTATTCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCATATGc                           >  1:878415/1‑66 (MQ=255)
aCGCCAAAATTATTCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCATATGc                           >  1:831528/1‑66 (MQ=255)
aCGCCAAAATTATTCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCATATGc                           >  1:809769/1‑66 (MQ=255)
aCGCCAAAATTATTCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCATATGc                           >  1:763756/1‑66 (MQ=255)
aCGCCAAAATTATTCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCATATGc                           >  1:727388/1‑66 (MQ=255)
aCGCCAAAATTATTCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCATATGc                           >  1:693390/1‑66 (MQ=255)
aCGCCAAAATTATTCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCATATGc                           >  1:690767/1‑66 (MQ=255)
aCGCCAAAATTATTCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCATATGc                           >  1:68829/1‑66 (MQ=255)
aCGCCAAAATTATTCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCATATGc                           >  1:1001092/1‑66 (MQ=255)
aCGCCAAAATTATTCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCATATGc                           >  1:252935/1‑66 (MQ=255)
aCGCCAAAATTATTCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCATATGc                           >  1:1469183/1‑66 (MQ=255)
aCGCCAAAATTATTCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCATATGc                           >  1:1646512/1‑66 (MQ=255)
aCGCCAAAATTATTCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCATATGc                           >  1:1748262/1‑66 (MQ=255)
aCGCCAAAATTATTCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCATATGc                           >  1:1806656/1‑66 (MQ=255)
aCGCCAAAATTATTCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCATATGc                           >  1:199319/1‑66 (MQ=255)
aCGCCAAAATTATTCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCATATGc                           >  1:2097481/1‑66 (MQ=255)
aCGCCAAAATTATTCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCATATGc                           >  1:2100318/1‑66 (MQ=255)
aCGCCAAAATTATTCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCATATGc                           >  1:2133733/1‑66 (MQ=255)
aCGCCAAAATTATTCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCATATGc                           >  1:244916/1‑66 (MQ=255)
aCGCCAAAATTATTCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCATATGc                           >  1:272268/1‑66 (MQ=255)
                         ccGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGa  <  1:658911/66‑1 (MQ=255)
                         ccGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGa  <  1:2142251/66‑1 (MQ=255)
                         ccGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGa  <  1:827693/66‑1 (MQ=255)
                         ccGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGa  <  1:345166/66‑1 (MQ=255)
                         ccGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGa  <  1:1582636/66‑1 (MQ=255)
                         ccGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGa  <  1:1238318/66‑1 (MQ=255)
                                               |                                           
ACGCCAAAATTATTCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGA  >  minE/1475618‑1475708

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑