| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,733,939 | A→T | 100% | Y159* (TAT→TAA) | pdxJ ← | pyridoxine 5'‑phosphate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,733,939 | 0 | A | T | 78.3% | 45.4 / 9.7 | 23 | Y159* (TAT→TAA) | pdxJ | pyridoxine 5'‑phosphate synthase |
| Reads supporting (aligned to +/- strand): ref base A (5/0); new base T (0/18); total (5/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.97e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACGGTGCGCCAACCTCTGCCGCAGCTTTGATCTGCTCTTC > minE/1733895‑1734004 | cgcgCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCGTCAGCTTAGCAACCGGTGTGGATCTCGa < 1:1763383/67‑1 (MQ=255)cgcgCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCTTAGCAACCGGTGTGGATCTCGa < 1:118555/67‑1 (MQ=255)cgcgCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCTTAGCAACCGGTGTGGATCTCGa < 1:499610/67‑1 (MQ=255)cgcgCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCTTAGCAACCGGTGTGGATCTCGa < 1:334089/67‑1 (MQ=255)cgcgCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCTTAGCAACCGGTGTGGATCTCGa < 1:2145069/67‑1 (MQ=255)cgcgCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCTTAGCAACCGGTGTGGATCTCGa < 1:2076087/67‑1 (MQ=255)cgcgCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCTTAGCAACCGGTGTGGATCTCGa < 1:1865085/67‑1 (MQ=255)cgcgCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCTTAGCAACCGGTGTGGATCTCGa < 1:1760945/67‑1 (MQ=255)cgcgCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCTTAGCAACCGGTGTGGATCTCGa < 1:1743550/67‑1 (MQ=255)cgcgCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCTTAGCAACCGGTGTGGATCTCGa < 1:1720150/67‑1 (MQ=255)cgcgCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCTTAGCAACCGGTGTGGATCTCGa < 1:1676571/67‑1 (MQ=255)cgcgCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCTTAGCAACCGGTGTGGATCTCGa < 1:1380964/67‑1 (MQ=255)cgcgCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCTTAGCAACCGGTGTGGATCTCGa < 1:1329961/67‑1 (MQ=255)cgcgCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCTTAGCAACCGGTGTGGATCTCGa < 1:119956/67‑1 (MQ=255)cgcgCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCTTAGCAACCGGTGTGGATCTCGa < 1:1164532/67‑1 (MQ=255)cgcgCCAGCTCTTGCGCCTGTTAGGCGTCAGTTTTGGCATCAGCTTAGCAACCGGTGTGGATCTCGa < 1:1636022/67‑1 (MQ=255) cTGTTCGGCGTCAGTTTTGGCATCAGCTTAGCAACCGGTGTGGATCTCGa < 1:1717564/50‑1 (MQ=255) ggCGTCAGTTTTGGCATCAGCTTAGCAACCGGTGTGGATCTCGa < 1:2142739/44‑1 (MQ=255) cATAGCAACCGGTGTGGATCTCGATAAACGGTGCGCCAACCTCTGCCGCAGCTTTGATCTGCTCTTc > 1:1441782/1‑67 (MQ=255) cATAGCAACCGGTGTGGATCTCGATAAACGGTGCGCCAACCTCTGCCGCAGCTTTGATCTGCTCTTc > 1:2006865/1‑67 (MQ=255) cATAGCAACCGGTGTGGATCTCGATAAACGGTGCGCCAACCTCTGCCGCAGCTTTGATCTGCTCTTc > 1:596535/1‑67 (MQ=255) cATAGCAACCGGTGTGGATCTCGATAAACGGTGCGCCAACCTCTGCCGCAGCTTTGATCTGCTCTTc > 1:690378/1‑67 (MQ=255) cATAGCAACCGGTGTGGATCTCGATAAACGGTGCGCCAACCTCTGCCGCAGCTTTGATCTGCTCTTc > 1:76153/1‑67 (MQ=255) | CGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACGGTGCGCCAACCTCTGCCGCAGCTTTGATCTGCTCTTC > minE/1733895‑1734004 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |