| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,790,195 | T→A | 100% | L14* (TTG→TAG) | gabD → | succinate‑semialdehyde dehydrogenase I, NADP‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,790,195 | 0 | T | A | 84.1% | 149.4 / 23.3 | 63 | L14* (TTG→TAG) | gabD | succinate‑semialdehyde dehydrogenase I, NADP‑dependent |
| Reads supporting (aligned to +/- strand): ref base T (0/10); new base A (53/0); total (53/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.82e-12 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.44e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GATGAAACTTAACGACAGTAACTTATTCCGCCAGCAGGCGTTGATTAACGGGGAATGGCTGGACGCCAACAATGGTGAAGCCATCGACGTCA > minE/1790154‑1790245 | gATGAAACTTAACGACAGTAACTTATTCCGCCAGCAGGCGTAGAtt > 1:1878891/1‑46 (MQ=255)gATGAAACTTAACGACAGTAACTTATTCCGCCAGCAGGCGTAGATTAACGGGGAATGGCTGGACGcc > 1:475061/1‑67 (MQ=255)gATGAAACTTAACGACAGTAACTTATTCCGCCAGCAGGCGTAGATTAACGGGGAATGGCTGGACGcc > 1:1964218/1‑67 (MQ=255)gATGAAACTTAACGACAGTAACTTATTCCGCCAGCAGGCGTAGATTAACGGGGAATGGCTGGACGcc > 1:1066270/1‑67 (MQ=255)gATGAAACTTAACGACAGTAACTTATTCCGCCAGCAGGCGTAGATTAACGGGGAATGGCTGGACGcc > 1:2117908/1‑67 (MQ=255)gATGAAACTTAACGACAGTAACTTATTCCGCCAGCAGGCGTAGATTAACGGGGAATGGCTGGACGcc > 1:2124694/1‑67 (MQ=255)gATGAAACTTAACGACAGTAACTTATTCCGCCAGCAGGCGTAGATTAACGGGGAATGGCTGGACGcc > 1:2150859/1‑67 (MQ=255)gATGAAACTTAACGACAGTAACTTATTCCGCCAGCAGGCGTAGATTAACGGGGAATGGCTGGACGcc > 1:250171/1‑67 (MQ=255)gATGAAACTTAACGACAGTAACTTATTCCGCCAGCAGGCGTAGATTAACGGGGAATGGCTGGACGcc > 1:306846/1‑67 (MQ=255)gATGAAACTTAACGACAGTAACTTATTCCGCCAGCAGGCGTAGATTAACGGGGAATGGCTGGACGcc > 1:322920/1‑67 (MQ=255)gATGAAACTTAACGACAGTAACTTATTCCGCCAGCAGGCGTAGATTAACGGGGAATGGCTGGACGcc > 1:330661/1‑67 (MQ=255)gATGAAACTTAACGACAGTAACTTATTCCGCCAGCAGGCGTAGATTAACGGGGAATGGCTGGACGcc > 1:368697/1‑67 (MQ=255)gATGAAACTTAACGACAGTAACTTATTCCGCCAGCAGGCGTAGATTAACGGGGAATGGCTGGACGcc > 1:37462/1‑67 (MQ=255)gATGAAACTTAACGACAGTAACTTATTCCGCCAGCAGGCGTAGATTAACGGGGAATGGCTGGACGcc > 1:389039/1‑67 (MQ=255)gATGAAACTTAACGACAGTAACTTATTCCGCCAGCAGGCGTAGATTAACGGGGAATGGCTGGACGcc > 1:400753/1‑67 (MQ=255)gATGAAACTTAACGACAGTAACTTATTCCGCCAGCAGGCGTAGATTAACGGGGAATGGCTGGACGcc > 1:20562/1‑67 (MQ=255)gATGAAACTTAACGACAGTAACTTATTCCGCCAGCAGGCGTAGATTAACGGGGAATGGCTGGACGcc > 1:48804/1‑67 (MQ=255)gATGAAACTTAACGACAGTAACTTATTCCGCCAGCAGGCGTAGATTAACGGGGAATGGCTGGACGcc > 1:526359/1‑67 (MQ=255)gATGAAACTTAACGACAGTAACTTATTCCGCCAGCAGGCGTAGATTAACGGGGAATGGCTGGACGcc > 1:535866/1‑67 (MQ=255)gATGAAACTTAACGACAGTAACTTATTCCGCCAGCAGGCGTAGATTAACGGGGAATGGCTGGACGcc > 1:621825/1‑67 (MQ=255)gATGAAACTTAACGACAGTAACTTATTCCGCCAGCAGGCGTAGATTAACGGGGAATGGCTGGACGcc > 1:629087/1‑67 (MQ=255)gATGAAACTTAACGACAGTAACTTATTCCGCCAGCAGGCGTAGATTAACGGGGAATGGCTGGACGcc > 1:638890/1‑67 (MQ=255)gATGAAACTTAACGACAGTAACTTATTCCGCCAGCAGGCGTAGATTAACGGGGAATGGCTGGACGcc > 1:664818/1‑67 (MQ=255)gATGAAACTTAACGACAGTAACTTATTCCGCCAGCAGGCGTAGATTAACGGGGAATGGCTGGACGcc > 1:695279/1‑67 (MQ=255)gATGAAACTTAACGACAGTAACTTATTCCGCCAGCAGGCGTAGATTAACGGGGAATGGCTGGACGcc > 1:753641/1‑67 (MQ=255)gATGAAACTTAACGACAGTAACTTATTCCGCCAGCAGGCGTAGATTAACGGGGAATGGCTGGACGcc > 1:814882/1‑67 (MQ=255)gATGAAACTTAACGACAGTAACTTATTCCGCCAGCAGGCGTAGATTAACGGGGAATGGCTGGACGcc > 1:899652/1‑67 (MQ=255)gATGAAACTTAACGACAGTAACTTATTCCGCCAGCAGGCGTAGATTAACGGGGAATGGCTGGACGcc > 1:977310/1‑67 (MQ=255)gATGAAACTTAACGACAGTAACTTATTCCGCCAGCAGGCGTAGATTAACGGGGAATGGCTGGACGcc > 1:986340/1‑67 (MQ=255)gATGAAACTTAACGACAGTAACTTATTCCGCCAGCAGGCGTAGATTAACGGGGAATGGCTGGACGcc > 1:1071648/1‑67 (MQ=255)gATGAAACTTAACGACAGTAACTTATTCCGCCAGCAGGCGTAGATTAACGGGGAATGGCTGGACGcc > 1:1254675/1‑67 (MQ=255)gATGAAACTTAACGACAGTAACTTATTCCGCCAGCAGGCGTAGATTAACGGGGAATGGCTGGACGcc > 1:1273603/1‑67 (MQ=255)gATGAAACTTAACGACAGTAACTTATTCCGCCAGCAGGCGTAGATTAACGGGGAATGGCTGGACGcc > 1:1192668/1‑67 (MQ=255)gATGAAACTTAACGACAGTAACTTATTCCGCCAGCAGGCGTAGATTAACGGGGAATGGCTGGACGcc > 1:1292837/1‑67 (MQ=255)gATGAAACTTAACGACAGTAACTTATTCCGCCAGCAGGCGTAGATTAACGGGGAATGGCTGGACGcc > 1:1180083/1‑67 (MQ=255)gATGAAACTTAACGACAGTAACTTATTCCGCCAGCAGGCGTAGATTAACGGGGAATGGCTGGACGcc > 1:1383812/1‑67 (MQ=255)gATGAAACTTAACGACAGTAACTTATTCCGCCAGCAGGCGTAGATTAACGGGGAATGGCTGGACGcc > 1:193418/1‑67 (MQ=255)gATGAAACTTAACGACAGTAACTTATTCCGCCAGCAGGCGTAGATTAACGGGGAATGGCTGGACGcc > 1:1418646/1‑67 (MQ=255)gATGAAACTTAACGACAGTAACTTATTCCGCCAGCAGGCGTAGATTAACGGGGAATGGCTGGACGcc > 1:148199/1‑67 (MQ=255)gATGAAACTTAACGACAGTAACTTATTCCGCCAGCAGGCGTAGATTAACGGGGAATGGCTGGACGcc > 1:1497324/1‑67 (MQ=255)gATGAAACTTAACGACAGTAACTTATTCCGCCAGCAGGCGTAGATTAACGGGGAATGGCTGGACGcc > 1:1512397/1‑67 (MQ=255)gATGAAACTTAACGACAGTAACTTATTCCGCCAGCAGGCGTAGATTAACGGGGAATGGCTGGACGcc > 1:1572192/1‑67 (MQ=255)gATGAAACTTAACGACAGTAACTTATTCCGCCAGCAGGCGTAGATTAACGGGGAATGGCTGGACGcc > 1:1572882/1‑67 (MQ=255)gATGAAACTTAACGACAGTAACTTATTCCGCCAGCAGGCGTAGATTAACGGGGAATGGCTGGACGcc > 1:1610205/1‑67 (MQ=255)gATGAAACTTAACGACAGTAACTTATTCCGCCAGCAGGCGTAGATTAACGGGGAATGGCTGGACGcc > 1:183015/1‑67 (MQ=255)gATGAAACTTAACGACAGTAACTTATTCCGCCAGCAGGCGTAGATTAACGGGGAATGGCTGGACGcc > 1:1893663/1‑67 (MQ=255)gATGAAACTTAACGACAGTAACTTATTCCGCCAGCAGGCGTAGATTAACGGGGAATGGCTGGACGcc > 1:1919062/1‑67 (MQ=255)gATGAAACTTAACGACAGTAACTTATTCCGCCAGCAGGCGTAGATTAACGGGGAATGGCTGGACGc > 1:1449509/1‑66 (MQ=255)gATGAAACTTAACGACAGTAACTTATTCCGCCAGCAGGCGTAGATTAACGGGGAATGGCTGGACGc > 1:827147/1‑66 (MQ=255)gATGAAACTTAACGACAGTAACTTATTCCGCCAGCAGGCGTAGATTAACGGGGAATGGCTGGACGc > 1:1341174/1‑66 (MQ=255)gATGAAACTTAACGACAGTAACTTATTCCGCCAGCAGGCGTAGATTAACGGGGAATGGCTGGACGc > 1:1301527/1‑66 (MQ=255)gATGAAACTTAACGACAGTAACTTATTCCGCCAGCAGGCGTAGATTAACGGGGAATGGCTGGACGc > 1:1072128/1‑66 (MQ=255)gATGAAACTTAACGACAGTAACTTATTCCGCCAGCAGGCGTAGATTAACGGGGAATGGCTGGACGc > 1:751906/1‑66 (MQ=255) tCCGCCAGCAGGCGTTGATTAACGGGGAATGGCTGGACGCCAACAATGGTGAAGCCATCGACGTCa < 1:1155062/66‑1 (MQ=255) tCCGCCAGCAGGCGTTGATTAACGGGGAATGGCTGGACGCCAACAATGGTGAAGCCATCGACGTCa < 1:924463/66‑1 (MQ=255) tCCGCCAGCAGGCGTTGATTAACGGGGAATGGCTGGACGCCAACAATGGTGAAGCCATCGACGTCa < 1:1418228/66‑1 (MQ=255) tCCGCCAGCAGGCGTTGATTAACGGGGAATGGCTGGACGCCAACAATGGTGAAGCCATCGACGTCa < 1:647824/66‑1 (MQ=255) tCCGCCAGCAGGCGTTGATTAACGGGGAATGGCTGGACGCCAACAATGGTGAAGCCATCGACGTCa < 1:1369466/66‑1 (MQ=255) tCCGCCAGCAGGCGTTGATTAACGGGGAATGGCTGGACGCCAACAATGGTGAAGCCATCGACGTCa < 1:483222/66‑1 (MQ=255) tCCGCCAGCAGGCGTTGATTAACGGGGAATGGCTGGACGCCAACAATGGTGAAGCCATCGACGTCa < 1:1880676/66‑1 (MQ=255) tCCGCCAGCAGGCGTTGATTAACGGGGAATGGCTGGACGCCAACAATGGTGAAGCCATCGACGTCa < 1:1926453/66‑1 (MQ=255) ccGCCAGCAGGCGTTGATTAACGGGGAATGGCTGGACGCCAACAATGGTGAAGCCATCGACGTCa < 1:180875/65‑1 (MQ=255) ccGCCAGCAGGCGTTGATTAACGGGGAATGGCTGGACGCCAACAATGGTGAAGCCATCGACGTCa < 1:214064/65‑1 (MQ=255) | GATGAAACTTAACGACAGTAACTTATTCCGCCAGCAGGCGTTGATTAACGGGGAATGGCTGGACGCCAACAATGGTGAAGCCATCGACGTCA > minE/1790154‑1790245 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |