| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,820,597 | T→A | 100% | intergenic (‑90/‑97) | norR ← / → norV | DNA‑binding transcriptional activator/flavorubredoxin oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,820,597 | 0 | T | A | 97.7% | 143.0 / ‑3.6 | 44 | intergenic (‑90/‑97) | norR/norV | DNA‑binding transcriptional activator/flavorubredoxin oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base A (43/0); total (43/1) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.27e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.36e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGA > minE/1820535‑1820656 | gATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATtttatt > 1:950806/1‑67 (MQ=255)gATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATtttatt > 1:2011995/1‑67 (MQ=255)gATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATtttatt > 1:1471737/1‑67 (MQ=255)gATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATtttatt > 1:173474/1‑67 (MQ=255)gATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATtttatt > 1:2014441/1‑67 (MQ=255) gACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTATATTTATAGAGTaaaaa > 1:971152/1‑66 (MQ=255) gACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTATATTTATAGAGTaaaaa > 1:1959350/1‑66 (MQ=255) gACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTATATTTATAGAGTaaaaa > 1:704174/1‑66 (MQ=255) gACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTATATTTATAGAGTaaaaa > 1:1870584/1‑66 (MQ=255) gACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTATATTTATAGAGTaaaaa > 1:62473/1‑66 (MQ=255) gACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTATATTTATAGAGTaaaaa > 1:1735847/1‑66 (MQ=255) gACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTATATTTATAGAGTaaaaa > 1:791698/1‑66 (MQ=255) gACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTATATTTATAGAGTaaaaa > 1:2129919/1‑66 (MQ=255) gACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTATATTTATAGAGTaaaaa > 1:226697/1‑66 (MQ=255) gACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTATATTTATAGAGTAAAAAc > 1:565614/1‑67 (MQ=255) gACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTATATTTATAGAGTAAAAAc > 1:550290/1‑67 (MQ=255) gACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTATATTTATAGAGTAAAAAc > 1:53254/1‑67 (MQ=255) gACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTATATTTATAGAGTAAAAAc > 1:2083389/1‑67 (MQ=255) gACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTATATTTATAGAGTAAAAAc > 1:51036/1‑67 (MQ=255) gACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTATATTTATAGAGTAAAAAc > 1:659497/1‑67 (MQ=255) gACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTATATTTATAGAGTAAAAAc > 1:763563/1‑67 (MQ=255) gACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTATATTTATAGAGTAAAAAc > 1:770182/1‑67 (MQ=255) gACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTATATTTATAGAGTAAAAAc > 1:780474/1‑67 (MQ=255) gACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTATATTTATAGAGTAAAAAc > 1:822565/1‑67 (MQ=255) gACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTATATTTATAGAGTAAAAAc > 1:866709/1‑67 (MQ=255) gACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTATATTTATAGAGTAAAAAc > 1:914315/1‑67 (MQ=255) gACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTATATTTATAGAGTAAAAAc > 1:93598/1‑67 (MQ=255) gACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTATATTTATAGAGTAAAAAc > 1:9459/1‑67 (MQ=255) gACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTATATTTATAGAGTAAAAAc > 1:963589/1‑67 (MQ=255) gACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTATATTTATAGAGTAAAAAc > 1:1671200/1‑67 (MQ=255) gACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTATATTTATAGAGTAAAAAc > 1:1193026/1‑67 (MQ=255) gACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTATATTTATAGAGTAAAAAc > 1:1203094/1‑67 (MQ=255) gACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTATATTTATAGAGTAAAAAc > 1:1208879/1‑67 (MQ=255) gACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTATATTTATAGAGTAAAAAc > 1:123232/1‑67 (MQ=255) gACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTATATTTATAGAGTAAAAAc > 1:1276731/1‑67 (MQ=255) gACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTATATTTATAGAGTAAAAAc > 1:1360468/1‑67 (MQ=255) gACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTATATTTATAGAGTAAAAAc > 1:1523778/1‑67 (MQ=255) gACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTATATTTATAGAGTAAAAAc > 1:1632351/1‑67 (MQ=255) gACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTATATTTATAGAGTAAAAAc > 1:1636026/1‑67 (MQ=255) gACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTATATTTATAGAGTAAAAAc > 1:1738579/1‑67 (MQ=255) gACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTATATTTATAGAGTAAAAAc > 1:1747161/1‑67 (MQ=255) gACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTATATTTATAGAGTAAAAAc > 1:1874230/1‑67 (MQ=255) gACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTATATTTATAGAGTAAAAAc > 1:1985410/1‑67 (MQ=255) gACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTATATTTATAGAGTAAAAAc > 1:199888/1‑67 (MQ=255) gACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTATATTTATAGAGTAAAAAc > 1:2066467/1‑67 (MQ=255) gACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTATATTTATAGAGTAAAAAc > 1:1003049/1‑67 (MQ=255) gACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTATATTTATAGAGTAAAAAc > 1:22962/1‑67 (MQ=255) gACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTATATTTATAGAGTAAAAAc > 1:400904/1‑67 (MQ=255) cATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGa < 1:420775/67‑1 (MQ=255) | GATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGA > minE/1820535‑1820656 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |