Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,890,961 G→A 100% P208S (CCG→TCG)  gudP ← predicted D‑glucarate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,890,9610GA100.0% 107.3 / NA 31P208S (CCG→TCG) gudPpredicted D‑glucarate transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/0);  total (31/0)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCT  >  minE/1890895‑1891003
                                                                  |                                          
gCTGATCCATATTGATCAGCGCTCCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACGCCCgg                                            >  1:185327/1‑67 (MQ=255)
gCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCgg                                            >  1:1952818/1‑67 (MQ=255)
gCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCgg                                            >  1:98740/1‑67 (MQ=255)
gCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCgg                                            >  1:978410/1‑67 (MQ=255)
gCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCgg                                            >  1:92552/1‑67 (MQ=255)
gCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCgg                                            >  1:921660/1‑67 (MQ=255)
gCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCgg                                            >  1:727900/1‑67 (MQ=255)
gCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCgg                                            >  1:658990/1‑67 (MQ=255)
gCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCgg                                            >  1:619029/1‑67 (MQ=255)
gCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCgg                                            >  1:565211/1‑67 (MQ=255)
gCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCgg                                            >  1:211466/1‑67 (MQ=255)
gCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCgg                                            >  1:2056068/1‑67 (MQ=255)
gCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCgg                                            >  1:2015622/1‑67 (MQ=255)
gCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCgg                                            >  1:1028767/1‑67 (MQ=255)
gCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCgg                                            >  1:1920164/1‑67 (MQ=255)
gCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCgg                                            >  1:1488217/1‑67 (MQ=255)
gCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCgg                                            >  1:1358461/1‑67 (MQ=255)
gCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCgg                                            >  1:1152860/1‑67 (MQ=255)
gCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCgg                                            >  1:1430621/1‑67 (MQ=255)
                                                     ttATTTACCCCCTAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCt  >  1:1129824/1‑56 (MQ=255)
                                                     ttATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGc   >  1:213701/1‑55 (MQ=255)
                                                     ttATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGc   >  1:573309/1‑55 (MQ=255)
                                                     ttATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCt  >  1:781056/1‑56 (MQ=255)
                                                     ttATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCt  >  1:1148932/1‑56 (MQ=255)
                                                     ttATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCt  >  1:1076512/1‑56 (MQ=255)
                                                     ttATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCt  >  1:654812/1‑56 (MQ=255)
                                                     ttATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCt  >  1:649467/1‑56 (MQ=255)
                                                     ttATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCt  >  1:1200430/1‑56 (MQ=255)
                                                     ttATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCt  >  1:1034413/1‑56 (MQ=255)
                                                     ttATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCt  >  1:983626/1‑56 (MQ=255)
                                                     ttATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCt  >  1:321293/1‑56 (MQ=255)
                                                     ttATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCt  >  1:261940/1‑56 (MQ=255)
                                                     ttATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCt  >  1:1031258/1‑56 (MQ=255)
                                                     ttATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCt  >  1:2132532/1‑56 (MQ=255)
                                                     ttATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCt  >  1:2154570/1‑56 (MQ=255)
                                                     ttATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCt  >  1:13318/1‑56 (MQ=255)
                                                     ttATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCt  >  1:2069716/1‑56 (MQ=255)
                                                     ttATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCt  >  1:1349906/1‑56 (MQ=255)
                                                     ttATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCt  >  1:1972278/1‑56 (MQ=255)
                                                     ttATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCt  >  1:1959235/1‑56 (MQ=255)
                                                     ttATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCt  >  1:1415108/1‑56 (MQ=255)
                                                     ttATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCt  >  1:1922141/1‑56 (MQ=255)
                                                     ttATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCt  >  1:1921760/1‑56 (MQ=255)
                                                     ttATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCt  >  1:1891213/1‑56 (MQ=255)
                                                     ttATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCt  >  1:1442150/1‑56 (MQ=255)
                                                     ttATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCt  >  1:1774260/1‑56 (MQ=255)
                                                     ttATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCt  >  1:1594435/1‑56 (MQ=255)
                                                     ttATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCt  >  1:1518851/1‑56 (MQ=255)
                                                     ttATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCt  >  1:1489162/1‑56 (MQ=255)
                                                     ttATTTACCCCCGAATGTTGATTTGGATCGTGGATGACTTTCAACCAGATAAAGCt  >  1:1238926/1‑56 (MQ=255)
                                                                  |                                          
GCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCT  >  minE/1890895‑1891003

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: