| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,935,566 | A→T | 100% | intergenic (‑16/+75) | prfB ← / ← recJ | peptide chain release factor RF‑2/ssDNA exonuclease, 5' ‑‑> 3'‑specific |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,935,566 | 0 | A | T | 100.0% | 37.8 / NA | 13 | intergenic (‑16/+75) | prfB/recJ | peptide chain release factor RF‑2/ssDNA exonuclease, 5' ‑‑> 3'‑specific |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (0/13); total (0/13) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch. | |||||||||||
TTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTC > minE/1935537‑1935577 | ttAATTTCAAACATGGTCTGATTTCTTTTTTTGAGCTAGTc < 1:1104746/41‑1 (MQ=255)ttAATTTCAAACATGGTCTGATTTCTTTTTTTGAGCTAGTc < 1:1139056/41‑1 (MQ=255)ttAATTTCAAACATGGTCTGATTTCTTTTTTTGAGCTAGTc < 1:1231093/41‑1 (MQ=255)ttAATTTCAAACATGGTCTGATTTCTTTTTTTGAGCTAGTc < 1:162992/41‑1 (MQ=255)ttAATTTCAAACATGGTCTGATTTCTTTTTTTGAGCTAGTc < 1:1792583/41‑1 (MQ=255)ttAATTTCAAACATGGTCTGATTTCTTTTTTTGAGCTAGTc < 1:301124/41‑1 (MQ=255)ttAATTTCAAACATGGTCTGATTTCTTTTTTTGAGCTAGTc < 1:518522/41‑1 (MQ=255)ttAATTTCAAACATGGTCTGATTTCTTTTTTTGAGCTAGTc < 1:601398/41‑1 (MQ=255)ttAATTTCAAACATGGTCTGATTTCTTTTTTTGAGCTAGTc < 1:631647/41‑1 (MQ=255)ttAATTTCAAACATGGTCTGATTTCTTTTTTTGAGCTAGTc < 1:835952/41‑1 (MQ=255)ttAATTTCAAACATGGTCTGATTTCTTTTTTTGAGCTAGTc < 1:935698/41‑1 (MQ=255) tAATTTCAAACATGGTCTGATTTCTTTTTTTGAGCTAGTc < 1:575717/40‑1 (MQ=255) aaTTTCAAACATGGTCTGATTTCTTTTTTTGAGCTAGTc < 1:714845/39‑1 (MQ=255) | TTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTC > minE/1935537‑1935577 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |