| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,939,251 | G→A | 100% | M43I (ATG→ATA) | fldB → | flavodoxin 2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,939,251 | 0 | G | A | 77.3% | 40.3 / 9.5 | 22 | M43I (ATG→ATA) | fldB | flavodoxin 2 |
| Reads supporting (aligned to +/- strand): ref base G (5/0); new base A (0/17); total (5/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.80e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
ATCGGCCCAGAACTGGTGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGAT > minE/1939195‑1939263 | atCGGCCCAGAACTGGTGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATAGatc < 1:1074476/61‑3 (MQ=255)atCGGCCCAGAACTGGTGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATAGatc < 1:89131/61‑3 (MQ=255)atCGGCCCAGAACTGGTGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATAGatc < 1:282735/61‑3 (MQ=255)atCGGCCCAGAACTGGTGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATAGatc < 1:210364/61‑3 (MQ=255)atCGGCCCAGAACTGGTGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATAGatc < 1:202167/61‑3 (MQ=255)atCGGCCCAGAACTGGTGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATAGatc < 1:197123/61‑3 (MQ=255)atCGGCCCAGAACTGGTGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATAGatc < 1:1966876/61‑3 (MQ=255)atCGGCCCAGAACTGGTGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATAGatc < 1:1793105/61‑3 (MQ=255)atCGGCCCAGAACTGGTGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATAGatc < 1:1783639/61‑3 (MQ=255)atCGGCCCAGAACTGGTGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATAGatc < 1:1711760/61‑3 (MQ=255)atCGGCCCAGAACTGGTGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATAGatc < 1:1690389/61‑3 (MQ=255)atCGGCCCAGAACTGGTGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATAGatc < 1:1516309/61‑3 (MQ=255) tCGGCCCAGAACTGGTGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATAGatc < 1:480221/60‑3 (MQ=255) tCGGCCCAGAACTGGTGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATAGatc < 1:982296/60‑3 (MQ=255) cGGCCCAGAACTGGTGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGAt > 1:1782567/1‑67 (MQ=255) cGGCCCAGAACTGGTGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGAt > 1:1287521/1‑67 (MQ=255) cGGCCCAGAACTGGTGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGAt > 1:1245756/1‑67 (MQ=255) cGGCCCAGAACTGGTGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGAt > 1:2051774/1‑67 (MQ=255) cGGCCCAGAACTGGTGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGAt > 1:1117283/1‑67 (MQ=255) aGAACTGGTGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATAGatc < 1:1138774/53‑3 (MQ=255) aCCTTACAAAACCTCAAGGACGACTCCCCGAAATTAATAGatc < 1:1289699/43‑3 (MQ=255) ccTTACATAACCTCAAGGACGACTCCCCGAAATTAATAGatc < 1:1074268/42‑3 (MQ=255) | ATCGGCCCAGAACTGGTGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGAT > minE/1939195‑1939263 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |