| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,236,903 | G→A | 69.2% | A150T (GCT→ACT) | purD → | phosphoribosylglycinamide synthetase phosphoribosylamine‑glycine ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,236,903 | 0 | G | A | 69.2% | 150.4 / 97.6 | 107 | A150T (GCT→ACT) | purD | phosphoribosylglycinamide synthetase phosphoribosylamine‑glycine ligase |
| Reads supporting (aligned to +/- strand): ref base G (4/29); new base A (42/32); total (46/61) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.46e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01 | |||||||||||
Only 100 of 107 total aligned reads displayed.
AACCTGCGCTGGCGTATCTGCGTGAGAAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAGAAGCGGAAGCG > minE/2236841‑2236953 | aaCCTGCGCTGGCGTATCTGCGTGAGAAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGGCTGc > 1:936209/1‑67 (MQ=255)aaCCTGCGCTGGCGTATCTGCGTGAGAAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGGCTGc > 1:238013/1‑67 (MQ=255)aaCCTGCGCTGGCGTATCTGCGTGAGAAAAGCGCGCCAATCGTCATTAAAGCGGACGGTCTGGCTg > 1:328364/1‑66 (MQ=255) tGAGAAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCg < 1:194181/67‑1 (MQ=255) tGAGAAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCg < 1:1579626/67‑1 (MQ=255) tGAGAAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCg < 1:1595536/67‑1 (MQ=255) tGAGAAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCg < 1:836677/67‑1 (MQ=255) tGAGAAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCg < 1:808169/67‑1 (MQ=255) tGAGAAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCg < 1:705636/67‑1 (MQ=255) tGAGAAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCg < 1:1795508/67‑1 (MQ=255) tGAGAAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCg < 1:1803465/67‑1 (MQ=255) tGAGAAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCg < 1:1829280/67‑1 (MQ=255) tGAGAAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCg < 1:1892128/67‑1 (MQ=255) tGAGAAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCg < 1:1011036/67‑1 (MQ=255) tGAGAAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCg < 1:642501/67‑1 (MQ=255) tGAGAAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCg < 1:2048616/67‑1 (MQ=255) tGAGAAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCg < 1:45833/67‑1 (MQ=255) tGAGAAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCg < 1:2117691/67‑1 (MQ=255) tGAGAAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCg < 1:402068/67‑1 (MQ=255) tGAGAAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCg < 1:28715/67‑1 (MQ=255) tGAGAAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCg < 1:388543/67‑1 (MQ=255) tGAGAAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCg < 1:387747/67‑1 (MQ=255) tGAGAAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCg < 1:1119478/67‑1 (MQ=255) tGAGAAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCg < 1:1282660/67‑1 (MQ=255) tGAGAAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCg < 1:1279305/67‑1 (MQ=255) tGAGAAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCg < 1:1245346/67‑1 (MQ=255) tGAGAAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCg < 1:967569/67‑1 (MQ=255) tGAGAAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCg < 1:1456173/67‑1 (MQ=255) tGAGAAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCg < 1:106865/67‑1 (MQ=255) tGAGAAAGGCGCGCCAATCGTCATTAAAGCGGACGGGCTGACTGCCGGGAAAGGCGTTATCGTGGCg < 1:507637/67‑1 (MQ=255) gagaAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCg < 1:211442/66‑1 (MQ=255) agaAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCg < 1:917957/65‑1 (MQ=255) aTTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGgaa < 1:364830/57‑1 (MQ=255) aTTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGgaa < 1:1068785/57‑1 (MQ=255) aTTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGgaa < 1:1947674/57‑1 (MQ=255) aTTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGgaa < 1:953572/57‑1 (MQ=255) aTTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGgaa < 1:63112/57‑1 (MQ=255) aTTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGgaa < 1:2000060/57‑1 (MQ=255) aTTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGgaa < 1:548252/57‑1 (MQ=255) aTTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGgaa < 1:1005769/57‑1 (MQ=255) aTTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGgaa < 1:2065338/57‑1 (MQ=255) aTTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGgaa < 1:1509467/57‑1 (MQ=255) aTTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGgaa < 1:2129321/57‑1 (MQ=255) aTTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGgaa < 1:415337/57‑1 (MQ=255) aTTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGgaa < 1:281640/57‑1 (MQ=255) aTTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGgaa < 1:1608582/57‑1 (MQ=255) aTTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGgaa < 1:1600966/57‑1 (MQ=255) aTTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGgaa < 1:812806/57‑1 (MQ=255) aTTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGgaa < 1:1392398/57‑1 (MQ=255) aTTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGgaa < 1:1402087/57‑1 (MQ=255) aTTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGgaa < 1:1670750/57‑1 (MQ=255) aTTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGgaa < 1:1388578/57‑1 (MQ=255) aTTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGgaa < 1:149342/57‑1 (MQ=255) aTTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGgaa < 1:1350560/57‑1 (MQ=255) aTTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGgaa < 1:1331211/57‑1 (MQ=255) aTTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGgaa < 1:1316258/57‑1 (MQ=255) aTTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGGGGCGATGACGCTGgaa < 1:2031262/57‑1 (MQ=255) ttAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGgaa < 1:744752/56‑1 (MQ=255) tAAAGCTGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAgaagcggaagcg > 1:1664440/1‑67 (MQ=255) tAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCg > 1:1117993/1‑43 (MQ=255) tAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCg > 1:1475716/1‑43 (MQ=255) tAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCGATGAc > 1:1961559/1‑48 (MQ=255) tAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAgaagcggaagcg > 1:928183/1‑67 (MQ=255) tAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAgaagcggaagcg > 1:838248/1‑67 (MQ=255) tAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAgaagcggaagcg > 1:436458/1‑67 (MQ=255) tAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAgaagcggaagcg > 1:990186/1‑67 (MQ=255) tAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAgaagcggaagcg > 1:435146/1‑67 (MQ=255) tAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAgaagcggaagcg > 1:658526/1‑67 (MQ=255) tAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAgaagcggaagcg > 1:775588/1‑67 (MQ=255) tAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAgaagcggaagcg > 1:771846/1‑67 (MQ=255) tAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAgaagcggaagcg > 1:945208/1‑67 (MQ=255) tAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAgaagcggaagcg > 1:680346/1‑67 (MQ=255) tAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAgaagcggaagcg > 1:1757922/1‑67 (MQ=255) tAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAgaagcggaagcg > 1:1018513/1‑67 (MQ=255) tAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAgaagcggaagcg > 1:109541/1‑67 (MQ=255) tAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAgaagcggaagcg > 1:1279573/1‑67 (MQ=255) tAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAgaagcggaagcg > 1:1377190/1‑67 (MQ=255) tAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAgaagcggaagcg > 1:1442075/1‑67 (MQ=255) tAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAgaagcggaagcg > 1:1510508/1‑67 (MQ=255) tAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAgaagcggaagcg > 1:15651/1‑67 (MQ=255) tAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAgaagcggaagcg > 1:1634473/1‑67 (MQ=255) tAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAgaagcggaagcg > 1:1646381/1‑67 (MQ=255) tAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAgaagcggaagcg > 1:1666424/1‑67 (MQ=255) tAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAgaagcggaagcg > 1:1666476/1‑67 (MQ=255) tAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAgaagcggaagcg > 1:1681841/1‑67 (MQ=255) tAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAgaagcggaagcg > 1:371213/1‑67 (MQ=255) tAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAgaagcggaagcg > 1:1896940/1‑67 (MQ=255) tAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAgaagcggaagcg > 1:1988061/1‑67 (MQ=255) tAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAgaagcggaagcg > 1:1988285/1‑67 (MQ=255) tAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAgaagcggaagcg > 1:2040155/1‑67 (MQ=255) tAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAgaagcggaagcg > 1:2066099/1‑67 (MQ=255) tAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAgaagcggaagcg > 1:2072987/1‑67 (MQ=255) tAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAgaagcggaagcg > 1:2106979/1‑67 (MQ=255) tAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAgaagcggaagcg > 1:23182/1‑67 (MQ=255) tAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAgaagcggaagcg > 1:277188/1‑67 (MQ=255) tAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAgaagcggaagcg > 1:311848/1‑67 (MQ=255) tAAAGCGGACGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAgaagcggaagc > 1:235955/1‑66 (MQ=255) tAAAGCGGACGGTCAGACTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAgaagcggaagc > 1:1894074/1‑66 (MQ=255) gACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGgaa < 1:1587945/48‑1 (MQ=255) aCGGTCTGACTGCCGGGAAAGGCGTTATCGTGGCg < 1:1792057/35‑1 (MQ=255) | AACCTGCGCTGGCGTATCTGCGTGAGAAAGGCGCGCCAATCGTCATTAAAGCGGACGGTCTGGCTGCCGGGAAAGGCGTTATCGTGGCGATGACGCTGGAAGAAGCGGAAGCG > minE/2236841‑2236953 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |