Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,349,130 C→T 100% E840K (GAA→AAA)  polA ← fused DNA polymerase I 5'‑>3' exonuclease, 3'‑>5' polymerase and 3'‑>5' exonuclease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,349,1300CT85.7% 52.8 / 2.8 21E840K (GAA→AAA) polAfused DNA polymerase I 5'‑>3' exonuclease, 3'‑>5' polymerase and 3'‑>5' exonuclease
Reads supporting (aligned to +/- strand):  ref base C (0/3);  new base T (0/18);  total (0/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AATGTCGGCGGCGGTTCCCTGCATTGGCGCGTTAATGGCTGCACGTTCAGCCGCTGCACGACGAGCACCATTGCTGGATTTGATATCCGGCAGATA  >  minE/2349083‑2349178
                                               |                                                
aaTGTCGGCGGCGGTTCCCTGCATTGGCGCGTTAATGGCTGCACGTTTAGCCGCTGCACGACGAGc                                <  1:2091923/66‑1 (MQ=255)
aaTGTCGGCGGCGGTTCCCTGCATTGGCGCGTTAATGGCTGCACGTTTAGCCGCTGCACGACGAGc                                <  1:872881/66‑1 (MQ=255)
aaTGTCGGCGGCGGTTCCCTGCATTGGCGCGTTAATGGCTGCACGTTTAGCCGCTGCACGACGAGc                                <  1:686597/66‑1 (MQ=255)
aaTGTCGGCGGCGGTTCCCTGCATTGGCGCGTTAATGGCTGCACGTTTAGCCGCTGCACGACGAGc                                <  1:620764/66‑1 (MQ=255)
aaTGTCGGCGGCGGTTCCCTGCATTGGCGCGTTAATGGCTGCACGTTTAGCCGCTGCACGACGAGc                                <  1:513664/66‑1 (MQ=255)
aaTGTCGGCGGCGGTTCCCTGCATTGGCGCGTTAATGGCTGCACGTTTAGCCGCTGCACGACGAGc                                <  1:504060/66‑1 (MQ=255)
aaTGTCGGCGGCGGTTCCCTGCATTGGCGCGTTAATGGCTGCACGTTTAGCCGCTGCACGACGAGc                                <  1:463996/66‑1 (MQ=255)
aaTGTCGGCGGCGGTTCCCTGCATTGGCGCGTTAATGGCTGCACGTTTAGCCGCTGCACGACGAGc                                <  1:443508/66‑1 (MQ=255)
aaTGTCGGCGGCGGTTCCCTGCATTGGCGCGTTAATGGCTGCACGTTTAGCCGCTGCACGACGAGc                                <  1:31519/66‑1 (MQ=255)
aaTGTCGGCGGCGGTTCCCTGCATTGGCGCGTTAATGGCTGCACGTTTAGCCGCTGCACGACGAGc                                <  1:2156730/66‑1 (MQ=255)
aaTGTCGGCGGCGGTTCCCTGCATTGGCGCGTTAATGGCTGCACGTTTAGCCGCTGCACGACGAGc                                <  1:1086356/66‑1 (MQ=255)
aaTGTCGGCGGCGGTTCCCTGCATTGGCGCGTTAATGGCTGCACGTTTAGCCGCTGCACGACGAGc                                <  1:2080470/66‑1 (MQ=255)
aaTGTCGGCGGCGGTTCCCTGCATTGGCGCGTTAATGGCTGCACGTTTAGCCGCTGCACGACGAGc                                <  1:1220302/66‑1 (MQ=255)
aaTGTCGGCGGCGGTTCCCTGCATTGGCGCGTTAATGGCTGCACGTTTAGCCGCTGCACGACGAGc                                <  1:1159928/66‑1 (MQ=255)
aaTGTCGGCGGCGGTTCCCTGCATTGGCGCGTTAATGGCTGCACGTTTAGCCGCTGCACGACGAGc                                <  1:1146088/66‑1 (MQ=255)
aaTGTCGGCGGCGGTTCCCTGCATTGGCGCGTTAATGGCTGCAAGTTTAGCCGCTGCACGACGAGc                                <  1:2056251/66‑1 (MQ=255)
 aTGTCGGCGGCGGTTCCCTGCATTGGCGCGTTAATGGCTGCACGTTTAGCCGCTGCACGACGAGc                                <  1:1264845/65‑1 (MQ=255)
                    gCATTGGCGCGTTAATGGCTGCACGTTTAGCCGCTGCACGACGAGc                                <  1:695229/46‑1 (MQ=255)
                             cgTTAATGGCTGCACGTTCAGCCGCTGCACGACGAGCACCATTGCTGGATTTGATATCCGGCAGATa  <  1:1706965/67‑1 (MQ=255)
                             cgTTAATGGCTGCACGTTCAGCCGCTGCACGACGAGCACCATTGCTGGATTTGATATCCGGCAGATa  <  1:1662822/67‑1 (MQ=255)
                             cgTTAATGGCTGCACGTTCAGCCGCTGCACGACGAGCACCATTGCTGGATTTGATATCCGGCAGATa  <  1:127145/67‑1 (MQ=255)
                                               |                                                
AATGTCGGCGGCGGTTCCCTGCATTGGCGCGTTAATGGCTGCACGTTCAGCCGCTGCACGACGAGCACCATTGCTGGATTTGATATCCGGCAGATA  >  minE/2349083‑2349178

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: