| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,513,416 | T→C | 100% | F203S (TTT→TCT) | gltS → | glutamate transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,513,416 | 0 | T | C | 100.0% | 32.9 / NA | 10 | F203S (TTT→TCT) | gltS | glutamate transporter |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (0/10); total (0/10) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AGTCCCGACGGCGTTTGAAAAGCCGGATGTGGGACGCATGATCACCTCGTTGGTG > minE/2513402‑2513456 | aGTCCCGACGGCGTCTGAAAAGCCGGATGTGGGACGCATGATCACCTCGTTGGTg < 1:1049699/55‑1 (MQ=255)aGTCCCGACGGCGTCTGAAAAGCCGGATGTGGGACGCATGATCACCTCGTTGGTg < 1:1116578/55‑1 (MQ=255)aGTCCCGACGGCGTCTGAAAAGCCGGATGTGGGACGCATGATCACCTCGTTGGTg < 1:1214055/55‑1 (MQ=255)aGTCCCGACGGCGTCTGAAAAGCCGGATGTGGGACGCATGATCACCTCGTTGGTg < 1:1783248/55‑1 (MQ=255)aGTCCCGACGGCGTCTGAAAAGCCGGATGTGGGACGCATGATCACCTCGTTGGTg < 1:1808965/55‑1 (MQ=255)aGTCCCGACGGCGTCTGAAAAGCCGGATGTGGGACGCATGATCACCTCGTTGGTg < 1:2005250/55‑1 (MQ=255)aGTCCCGACGGCGTCTGAAAAGCCGGATGTGGGACGCATGATCACCTCGTTGGTg < 1:2014993/55‑1 (MQ=255)aGTCCCGACGGCGTCTGAAAAGCCGGATGTGGGACGCATGATCACCTCGTTGGTg < 1:2049539/55‑1 (MQ=255)aGTCCCGACGGCGTCTGAAAAGCCGGATGTGGGACGCATGATCACCTCGTTGGTg < 1:349280/55‑1 (MQ=255)aGTCCCGACGGCGTCTGAAAAGCCGGATGTGGGACGCATGATCACCTCGTTGGTg < 1:681479/55‑1 (MQ=255) | AGTCCCGACGGCGTTTGAAAAGCCGGATGTGGGACGCATGATCACCTCGTTGGTG > minE/2513402‑2513456 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |