| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,524,368 | G→T | 100% | A144E (GCG→GAG) | yicC ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,524,368 | 0 | G | T | 100.0% | 71.3 / NA | 20 | A144E (GCG→GAG) | yicC | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (20/0); total (20/0) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AATGCTTTCAGTGCCTGACCTTCGGTTTCGCGCGCGACAATAAAGTCGTCCAGCGTACCATCCAGCG > minE/2524335‑2524401 | aaTGCTTTCAGTGCCTGACCTTCGGTTTCGCGCTTGACAATAAAGTCGTCCAGCGTACCATCCAgcg > 1:1119942/1‑67 (MQ=255)aaTGCTTTCAGTGCCTGACCTTCGGTTTCGCGCTCGACAATaa > 1:1870785/1‑43 (MQ=255)aaTGCTTTCAGTGCCTGACCTTCGGTTTCGCGCTCGACAATAAAGTCGTCCAGCGTACCATCCAgcg > 1:1054583/1‑67 (MQ=255)aaTGCTTTCAGTGCCTGACCTTCGGTTTCGCGCTCGACAATAAAGTCGTCCAGCGTACCATCCAgcg > 1:832945/1‑67 (MQ=255)aaTGCTTTCAGTGCCTGACCTTCGGTTTCGCGCTCGACAATAAAGTCGTCCAGCGTACCATCCAgcg > 1:573623/1‑67 (MQ=255)aaTGCTTTCAGTGCCTGACCTTCGGTTTCGCGCTCGACAATAAAGTCGTCCAGCGTACCATCCAgcg > 1:2058817/1‑67 (MQ=255)aaTGCTTTCAGTGCCTGACCTTCGGTTTCGCGCTCGACAATAAAGTCGTCCAGCGTACCATCCAgcg > 1:1903646/1‑67 (MQ=255)aaTGCTTTCAGTGCCTGACCTTCGGTTTCGCGCTCGACAATAAAGTCGTCCAGCGTACCATCCAgcg > 1:1813454/1‑67 (MQ=255)aaTGCTTTCAGTGCCTGACCTTCGGTTTCGCGCTCGACAATAAAGTCGTCCAGCGTACCATCCAgcg > 1:1782345/1‑67 (MQ=255)aaTGCTTTCAGTGCCTGACCTTCGGTTTCGCGCTCGACAATAAAGTCGTCCAGCGTACCATCCAgcg > 1:1775739/1‑67 (MQ=255)aaTGCTTTCAGTGCCTGACCTTCGGTTTCGCGCTCGACAATAAAGTCGTCCAGCGTACCATCCAgcg > 1:1663222/1‑67 (MQ=255)aaTGCTTTCAGTGCCTGACCTTCGGTTTCGCGCTCGACAATAAAGTCGTCCAGCGTACCATCCAgcg > 1:1568764/1‑67 (MQ=255)aaTGCTTTCAGTGCCTGACCTTCGGTTTCGCGCTCGACAATAAAGTCGTCCAGCGTACCATCCAgcg > 1:1337704/1‑67 (MQ=255)aaTGCTTTCAGTGCCTGACCTTCGGTTTCGCGCTCGACAATAAAGTCGTCCAGCGTACCATCCAgc > 1:1696508/1‑66 (MQ=255)aaTGCTTTCAGTGCCTGACCTTCGGTTTCGCGCTCGACAATAAAGTCGTCCAGCGTACCATCCAgc > 1:1219628/1‑66 (MQ=255)aaTGCTTTCAGTGCCTGACCTTCGGTTTCGCGCTCGACAATAAAGTCGTCCAGCGTACCATCCAgc > 1:1055735/1‑66 (MQ=255)aaTGCTTTCAGTGCCTGACCTTCGGTTTCGCGCTCGACAATAAAGTCGTCCAGCGTACCATCCAgc > 1:66390/1‑66 (MQ=255)aaTGCTTTCAGTGCCTGACCTTCGGTTTCGCGCTCGACAATAAAGTCGTCCAGCGTACCATCCAgc > 1:827625/1‑66 (MQ=255)aaTGCTTTCAGTGCCTGACCTTCGGTTTCGCGCTCGACAATAAAGTCGTCCAGCGTACCATCCAgc > 1:1040365/1‑66 (MQ=255)aaTGCTTTCAGTGCCTGACCTTCGGTTTCGCGCTCGACAATAAAGTCGTCCAGCGTACCATCCAgc > 1:846122/1‑66 (MQ=255) | AATGCTTTCAGTGCCTGACCTTCGGTTTCGCGCGCGACAATAAAGTCGTCCAGCGTACCATCCAGCG > minE/2524335‑2524401 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |