Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,651,936	C→T	100%	A57V (GCG→GTG)	glgC →	glucose‑1‑phosphate adenylyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,651,936	0	C	T	100.0%	57.4 / NA	18	A57V (GCG→GTG)	glgC	glucose‑1‑phosphate adenylyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (0/18);  total (0/18)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GGTAAGTTCCGCATTATCGACTTTGCGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGCG  >  minE/2651911‑2651977                          |                                          ggTAAGTTCCGCATTATCGACTTTGTGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGCg  <  1:1640564/67‑1 (MQ=255) ggTAAGTTCCGCATTATCGACTTTGTGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGCg  <  1:934043/67‑1 (MQ=255) ggTAAGTTCCGCATTATCGACTTTGTGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGCg  <  1:891489/67‑1 (MQ=255) ggTAAGTTCCGCATTATCGACTTTGTGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGCg  <  1:859263/67‑1 (MQ=255) ggTAAGTTCCGCATTATCGACTTTGTGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGCg  <  1:847280/67‑1 (MQ=255) ggTAAGTTCCGCATTATCGACTTTGTGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGCg  <  1:463330/67‑1 (MQ=255) ggTAAGTTCCGCATTATCGACTTTGTGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGCg  <  1:2135610/67‑1 (MQ=255) ggTAAGTTCCGCATTATCGACTTTGTGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGCg  <  1:2115316/67‑1 (MQ=255) ggTAAGTTCCGCATTATCGACTTTGTGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGCg  <  1:186272/67‑1 (MQ=255) ggTAAGTTCCGCATTATCGACTTTGTGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGCg  <  1:1039951/67‑1 (MQ=255) ggTAAGTTCCGCATTATCGACTTTGTGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGCg  <  1:148301/67‑1 (MQ=255) ggTAAGTTCCGCATTATCGACTTTGTGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGCg  <  1:1319010/67‑1 (MQ=255) ggTAAGTTCCGCATTATCGACTTTGTGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGCg  <  1:13040/67‑1 (MQ=255) ggTAAGTTCCGCATTATCGACTTTGTGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGCg  <  1:1237754/67‑1 (MQ=255) ggTAAGTTCCGCATTATCGACTTTGTGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGCg  <  1:1228642/67‑1 (MQ=255) ggTAAGTTCCGCATTATCGACTTTGTGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGCg  <  1:114856/67‑1 (MQ=255) ggTAAGTTCCGCATTATCGACTTTGTGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGCg  <  1:1057398/67‑1 (MQ=255)   tAAGTTCCGCATTATCGACTTTGTGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGCg  <  1:1061060/65‑1 (MQ=255)                          |                                          GGTAAGTTCCGCATTATCGACTTTGCGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGCG  >  minE/2651911‑2651977

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑