| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,656,124 | G→T | 100% | V537V (GTG→GTT) | glgP → | glycogen phosphorylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,656,124 | 0 | G | T | 100.0% | 12.7 / NA | 5 | V537V (GTG→GTT) | glgP | glycogen phosphorylase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (5/0); total (5/0) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
ATATCGCCCAGCAGCTGAATGTGGTGGTGAATCCAAAGGCGTTGTTCGATGTACAAATCAAACGTA > minE/2656102‑2656167 | atatCGCCCAGCAGCTGAATGTTGTGGTGAATCCAAAGGCGTTGTTCGATGTACAAATCAAACGt > 1:123936/1‑65 (MQ=255)atatCGCCCAGCAGCTGAATGTTGTGGTGAATCCAAAGGCGTTGTTCGATGTACAAATCAAACGTa > 1:348736/1‑66 (MQ=255)atatCGCCCAGCAGCTGAATGTTGTGGTGAATCCAAAGGCGTTGTTCGATGTACAAATCAAACGTa > 1:870445/1‑66 (MQ=255)atatCGCCCAGCAGCTGAATGTTGTGGTGAATCCAAAGGCGTTGTTCGATGTACAAATCAAACGTa > 1:872976/1‑66 (MQ=255)atatCGCCCAGCAGCTGAATGTTGTGGTGAATCCAAAGGCGTTGTTCGATGTACAAATCAAACGTa > 1:975451/1‑66 (MQ=255) | ATATCGCCCAGCAGCTGAATGTGGTGGTGAATCCAAAGGCGTTGTTCGATGTACAAATCAAACGTA > minE/2656102‑2656167 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |