Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,682,133	T→A	100%	N527Y (AAC→TAC)	yhgF ←	predicted transcriptional accessory protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,682,133	0	T	A	100.0%	131.4 / NA	38	N527Y (AAC→TAC)	yhgF	predicted transcriptional accessory protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (38/0);  total (38/0)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TAACAGTTGCTGACGGTTCTGGAACTGACCGTTCTCATCGCGCCAGGCAACGATGTTTTGCGCC  >  minE/2682101‑2682164                                 |                                tAACAGTTGCTGACGGTTCTGGAACTGACCGTACTCATcgc                         >  1:2010829/1‑41 (MQ=255) tAACAGTTGCTGACGGTTCTGGAACTGACCGTACTCATCGCGCCAGGCAACGATGTTTTGCGcc  >  1:566348/1‑64 (MQ=255) tAACAGTTGCTGACGGTTCTGGAACTGACCGTACTCATCGCGCCAGGCAACGATGTTTTGCGcc  >  1:330437/1‑64 (MQ=255) tAACAGTTGCTGACGGTTCTGGAACTGACCGTACTCATCGCGCCAGGCAACGATGTTTTGCGcc  >  1:376857/1‑64 (MQ=255) tAACAGTTGCTGACGGTTCTGGAACTGACCGTACTCATCGCGCCAGGCAACGATGTTTTGCGcc  >  1:407350/1‑64 (MQ=255) tAACAGTTGCTGACGGTTCTGGAACTGACCGTACTCATCGCGCCAGGCAACGATGTTTTGCGcc  >  1:409775/1‑64 (MQ=255) tAACAGTTGCTGACGGTTCTGGAACTGACCGTACTCATCGCGCCAGGCAACGATGTTTTGCGcc  >  1:45860/1‑64 (MQ=255) tAACAGTTGCTGACGGTTCTGGAACTGACCGTACTCATCGCGCCAGGCAACGATGTTTTGCGcc  >  1:47190/1‑64 (MQ=255) tAACAGTTGCTGACGGTTCTGGAACTGACCGTACTCATCGCGCCAGGCAACGATGTTTTGCGcc  >  1:485338/1‑64 (MQ=255) tAACAGTTGCTGACGGTTCTGGAACTGACCGTACTCATCGCGCCAGGCAACGATGTTTTGCGcc  >  1:51098/1‑64 (MQ=255) tAACAGTTGCTGACGGTTCTGGAACTGACCGTACTCATCGCGCCAGGCAACGATGTTTTGCGcc  >  1:241332/1‑64 (MQ=255) tAACAGTTGCTGACGGTTCTGGAACTGACCGTACTCATCGCGCCAGGCAACGATGTTTTGCGcc  >  1:621613/1‑64 (MQ=255) tAACAGTTGCTGACGGTTCTGGAACTGACCGTACTCATCGCGCCAGGCAACGATGTTTTGCGcc  >  1:670657/1‑64 (MQ=255) tAACAGTTGCTGACGGTTCTGGAACTGACCGTACTCATCGCGCCAGGCAACGATGTTTTGCGcc  >  1:707211/1‑64 (MQ=255) tAACAGTTGCTGACGGTTCTGGAACTGACCGTACTCATCGCGCCAGGCAACGATGTTTTGCGcc  >  1:766223/1‑64 (MQ=255) tAACAGTTGCTGACGGTTCTGGAACTGACCGTACTCATCGCGCCAGGCAACGATGTTTTGCGcc  >  1:776168/1‑64 (MQ=255) tAACAGTTGCTGACGGTTCTGGAACTGACCGTACTCATCGCGCCAGGCAACGATGTTTTGCGcc  >  1:861523/1‑64 (MQ=255) tAACAGTTGCTGACGGTTCTGGAACTGACCGTACTCATCGCGCCAGGCAACGATGTTTTGCGcc  >  1:875353/1‑64 (MQ=255) tAACAGTTGCTGACGGTTCTGGAACTGACCGTACTCATCGCGCCAGGCAACGATGTTTTGCGcc  >  1:93460/1‑64 (MQ=255) tAACAGTTGCTGACGGTTCTGGAACTGACCGTACTCATCGCGCCAGGCAACGATGTTTTGCGcc  >  1:987966/1‑64 (MQ=255) tAACAGTTGCTGACGGTTCTGGAACTGACCGTACTCATCGCGCCAGGCAACGATGTTTTGCGcc  >  1:1706445/1‑64 (MQ=255) tAACAGTTGCTGACGGTTCTGGAACTGACCGTACTCATCGCGCCAGGCAACGATGTTTTGCGcc  >  1:1247284/1‑64 (MQ=255) tAACAGTTGCTGACGGTTCTGGAACTGACCGTACTCATCGCGCCAGGCAACGATGTTTTGCGcc  >  1:1450533/1‑64 (MQ=255) tAACAGTTGCTGACGGTTCTGGAACTGACCGTACTCATCGCGCCAGGCAACGATGTTTTGCGcc  >  1:1480719/1‑64 (MQ=255) tAACAGTTGCTGACGGTTCTGGAACTGACCGTACTCATCGCGCCAGGCAACGATGTTTTGCGcc  >  1:1503205/1‑64 (MQ=255) tAACAGTTGCTGACGGTTCTGGAACTGACCGTACTCATCGCGCCAGGCAACGATGTTTTGCGcc  >  1:154468/1‑64 (MQ=255) tAACAGTTGCTGACGGTTCTGGAACTGACCGTACTCATCGCGCCAGGCAACGATGTTTTGCGcc  >  1:1590053/1‑64 (MQ=255) tAACAGTTGCTGACGGTTCTGGAACTGACCGTACTCATCGCGCCAGGCAACGATGTTTTGCGcc  >  1:1664189/1‑64 (MQ=255) tAACAGTTGCTGACGGTTCTGGAACTGACCGTACTCATCGCGCCAGGCAACGATGTTTTGCGcc  >  1:1155831/1‑64 (MQ=255) tAACAGTTGCTGACGGTTCTGGAACTGACCGTACTCATCGCGCCAGGCAACGATGTTTTGCGcc  >  1:1742959/1‑64 (MQ=255) tAACAGTTGCTGACGGTTCTGGAACTGACCGTACTCATCGCGCCAGGCAACGATGTTTTGCGcc  >  1:1766107/1‑64 (MQ=255) tAACAGTTGCTGACGGTTCTGGAACTGACCGTACTCATCGCGCCAGGCAACGATGTTTTGCGcc  >  1:1775139/1‑64 (MQ=255) tAACAGTTGCTGACGGTTCTGGAACTGACCGTACTCATCGCGCCAGGCAACGATGTTTTGCGcc  >  1:1818054/1‑64 (MQ=255) tAACAGTTGCTGACGGTTCTGGAACTGACCGTACTCATCGCGCCAGGCAACGATGTTTTGCGcc  >  1:1846519/1‑64 (MQ=255) tAACAGTTGCTGACGGTTCTGGAACTGACCGTACTCATCGCGCCAGGCAACGATGTTTTGCGcc  >  1:1901853/1‑64 (MQ=255) tAACAGTTGCTGACGGTTCTGGAACTGACCGTACTCATCGCGCCAGGCAACGATGTTTTGCGcc  >  1:1939540/1‑64 (MQ=255) tAACAGTTGCTGACGGTTCTGGAACTGACCGTACTCATCGCGCCAGGCAACGATGTTTTGCGcc  >  1:2106801/1‑64 (MQ=255) tAACAGTTGCTGACGGTTCTGGAACTGACCGTACTCATAGCGCCAGGCAACGATGTTTTGCGcc  >  1:1588627/1‑64 (MQ=255)                                 |                                TAACAGTTGCTGACGGTTCTGGAACTGACCGTTCTCATCGCGCCAGGCAACGATGTTTTGCGCC  >  minE/2682101‑2682164

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑