| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,687,159 | G→A | 100% | L374L (CTG→TTG) | pck ← | phosphoenolpyruvate carboxykinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,687,159 | 0 | G | A | 100.0% | 15.5 / NA | 6 | L374L (CTG→TTG) | pck | phosphoenolpyruvate carboxykinase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (6/0); total (6/0) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGAAAGC > minE/2687141‑2687189 | gggTTTGATCGGCAGTCAAGCGAGAAACCGGCGGCAACACg > 1:1679614/1‑41 (MQ=255)gggTTTGATCGGCAGTCAAGCGAGAAACCGGCGGCAACACGCCGAAAGc > 1:1205766/1‑49 (MQ=255)gggTTTGATCGGCAGTCAAGCGAGAAACCGGCGGCAACACGCCGAAAGc > 1:1237717/1‑49 (MQ=255)gggTTTGATCGGCAGTCAAGCGAGAAACCGGCGGCAACACGCCGAAAGc > 1:1746779/1‑49 (MQ=255)gggTTTGATCGGCAGTCAAGCGAGAAACCGGCGGCAACACGCCGAAAGc > 1:751250/1‑49 (MQ=255)gggTTTGATCGGCAGTCAAGCGAGAAACCGGCGGCAACACGCCGAAAGc > 1:855108/1‑49 (MQ=255) | GGGTTTGATCGGCAGTCAGGCGAGAAACCGGCGGCAACACGCCGAAAGC > minE/2687141‑2687189 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |