Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,787,894	T→G	100%	I180L (ATC→CTC)	lysC ←	aspartokinase III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,787,894	0	T	G	100.0%	48.4 / NA	14	I180L (ATC→CTC)	lysC	aspartokinase III
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (14/0);  total (14/0)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TGCCTCCACGGCCAAGCGTCGTTGTACGACCTTTATTTTCGCTACCGATAAATCCCTGGGTGATCAC  >  minE/2787831‑2787897                                                                |    tGCCTCCACGGCCAAGCGTCGTTGTACGACCTTTATTTTCGCTACCGATAAATCCCTGGGTGAGCAc  >  1:285706/1‑67 (MQ=255) tGCCTCCACGGCCAAGCGTCGTTGTACGACCTTTATTTTCGCTACCGATAAATCCCTGGGAGAGCa   >  1:1146965/1‑66 (MQ=255) tGCCTCCACGGCCAAGCGTCGTTGTACGACCTTTATTTTCGCTACCGATAAATCCCTGGGAGAGCAc  >  1:1063130/1‑67 (MQ=255) tGCCTCCACGGCCAAGCGTCGTTGTACGACCTTTATTTTCGCTACCGATAAATCCCTGGGAGAGCAc  >  1:1208320/1‑67 (MQ=255) tGCCTCCACGGCCAAGCGTCGTTGTACGACCTTTATTTTCGCTACCGATAAATCCCTGGGAGAGCAc  >  1:1383258/1‑67 (MQ=255) tGCCTCCACGGCCAAGCGTCGTTGTACGACCTTTATTTTCGCTACCGATAAATCCCTGGGAGAGCAc  >  1:1613040/1‑67 (MQ=255) tGCCTCCACGGCCAAGCGTCGTTGTACGACCTTTATTTTCGCTACCGATAAATCCCTGGGAGAGCAc  >  1:1816470/1‑67 (MQ=255) tGCCTCCACGGCCAAGCGTCGTTGTACGACCTTTATTTTCGCTACCGATAAATCCCTGGGAGAGCAc  >  1:186664/1‑67 (MQ=255) tGCCTCCACGGCCAAGCGTCGTTGTACGACCTTTATTTTCGCTACCGATAAATCCCTGGGAGAGCAc  >  1:1964990/1‑67 (MQ=255) tGCCTCCACGGCCAAGCGTCGTTGTACGACCTTTATTTTCGCTACCGATAAATCCCTGGGAGAGCAc  >  1:2114994/1‑67 (MQ=255) tGCCTCCACGGCCAAGCGTCGTTGTACGACCTTTATTTTCGCTACCGATAAATCCCTGGGAGAGCAc  >  1:242076/1‑67 (MQ=255) tGCCTCCACGGCCAAGCGTCGTTGTACGACCTTTATTTTCGCTACCGATAAATCCCTGGGAGAGCAc  >  1:642655/1‑67 (MQ=255) tGCCTCCACGGCCAAGCGTCGTTGTACGACCTTTATTTTCGCTACCGATAAATCCCTGGGAGAGCAc  >  1:865130/1‑67 (MQ=255) tGCCACCACGGCCAAGCGTCGTTGTACGACCTTTATTTTCGCTACCGATAAATCCCTGGGAGAGCa   >  1:1322362/1‑66 (MQ=255)                                                                |    TGCCTCCACGGCCAAGCGTCGTTGTACGACCTTTATTTTCGCTACCGATAAATCCCTGGGTGATCAC  >  minE/2787831‑2787897

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑