| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,948,123 | T→C | 25.8% | T40A (ACC→GCC) | yjjB ← | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,948,123 | 0 | T | C | 25.8% | 167.3 / 67.0 | 93 | T40A (ACC→GCC) | yjjB | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (26/43); new base C (20/4); total (46/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.22e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCGCCGAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGT > minE/2948060‑2948174 | ccgccgAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTaa < 1:255853/66‑1 (MQ=255)ccgccgAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTaa < 1:1511653/66‑1 (MQ=255)ccgccgAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTaa < 1:1535561/66‑1 (MQ=255)ccgccgAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTaa < 1:1641932/66‑1 (MQ=255)ccgccgAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTaa < 1:1649753/66‑1 (MQ=255)ccgccgAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTaa < 1:1678030/66‑1 (MQ=255)ccgccgAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTaa < 1:177446/66‑1 (MQ=255)ccgccgAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTaa < 1:1831718/66‑1 (MQ=255)ccgccgAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTaa < 1:191382/66‑1 (MQ=255)ccgccgAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTaa < 1:1953312/66‑1 (MQ=255)ccgccgAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTaa < 1:1958784/66‑1 (MQ=255)ccgccgAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTaa < 1:1985195/66‑1 (MQ=255)ccgccgAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTaa < 1:2048580/66‑1 (MQ=255)ccgccgAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTaa < 1:2110937/66‑1 (MQ=255)ccgccgAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTaa < 1:2119345/66‑1 (MQ=255)ccgccgAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTaa < 1:102045/66‑1 (MQ=255)ccgccgAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTaa < 1:291464/66‑1 (MQ=255)ccgccgAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTaa < 1:346079/66‑1 (MQ=255)ccgccgAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTaa < 1:368554/66‑1 (MQ=255)ccgccgAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTaa < 1:455650/66‑1 (MQ=255)ccgccgAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTaa < 1:557963/66‑1 (MQ=255)ccgccgAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTaa < 1:600747/66‑1 (MQ=255)ccgccgAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTaa < 1:651422/66‑1 (MQ=255)ccgccgAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTaa < 1:761400/66‑1 (MQ=255)ccgccgAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTaa < 1:770232/66‑1 (MQ=255)ccgccgAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTaa < 1:902801/66‑1 (MQ=255)ccgccgAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTaa < 1:907265/66‑1 (MQ=255)ccgccgAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTaa < 1:912230/66‑1 (MQ=255)ccgccgAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTaa < 1:997408/66‑1 (MQ=255)ccgccgAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTaa < 1:100535/66‑1 (MQ=255)ccgccgAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTaa < 1:1046010/66‑1 (MQ=255)ccgccgAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTaa < 1:1057541/66‑1 (MQ=255)ccgccgAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTaa < 1:108692/66‑1 (MQ=255)ccgccgAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTaa < 1:1432029/66‑1 (MQ=255)ccgccgAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTaa < 1:1116576/66‑1 (MQ=255)ccgccgAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTaa < 1:1217900/66‑1 (MQ=255)ccgccgAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTaa < 1:1242494/66‑1 (MQ=255)ccgccgAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTaa < 1:1382445/66‑1 (MQ=255)ccgccgAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTaa < 1:124274/66‑1 (MQ=255)ccgccgAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTaa < 1:1334731/66‑1 (MQ=255)ccgccgAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTaa < 1:1268213/66‑1 (MQ=255) cgccgAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTaa < 1:188917/65‑1 (MQ=255) ttGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTaa < 1:2041144/54‑1 (MQ=255) ccGTTATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATAcc > 1:295949/1‑66 (MQ=255) ccGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATAcc > 1:865778/1‑66 (MQ=255) ccGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATAcc > 1:1725247/1‑66 (MQ=255) ccGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCa > 1:1325415/1‑67 (MQ=255) ccGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCa > 1:651154/1‑67 (MQ=255) ccGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCa > 1:353485/1‑67 (MQ=255) ccGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCa > 1:1490763/1‑67 (MQ=255) ccGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCa > 1:1208430/1‑67 (MQ=255) ccGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCa > 1:2131951/1‑67 (MQ=255) ccGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGCAAACACTTTCGGATgcgc > 1:571211/1‑58 (MQ=255) ccGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGCAAACACTTTCGGATGCGCCAGATAcc > 1:1079007/1‑66 (MQ=255) ccGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGCAAACACTTTCGGATGCGCCAGATAcc > 1:1219184/1‑66 (MQ=255) ccGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGCAAACACTTTCGGATGCGCCAGATAcc > 1:997120/1‑66 (MQ=255) ccGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGCAAACACTTTCGGATGCGCCAGATAcc > 1:849110/1‑66 (MQ=255) ccGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGCAAACACTTTCGGATGCGCCAGATAcc > 1:442521/1‑66 (MQ=255) ccGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGCAAACACTTTCGGATGCGCCAGATAcc > 1:148462/1‑66 (MQ=255) ccGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGCAAACACTTTCGGATGCGCCAGATAcc > 1:1527802/1‑66 (MQ=255) ccGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGCAAACACTTTCGGATGCGCCAGATACCa > 1:1567153/1‑67 (MQ=255) ccGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGCAAACACTTTCGGATGCGCCAGATACCa > 1:715801/1‑67 (MQ=255) ccGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGCAAACACTTTCGGATGCGCCAGATACCa > 1:1060118/1‑67 (MQ=255) ccGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGCAAACACTTTCGGATGCGCCAGATACCa > 1:84806/1‑67 (MQ=255) ccGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGCAAACACTTTCGGATGCGCCAGATACCa > 1:1931535/1‑67 (MQ=255) ccGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGCAAACACTTTCGGATGCGCCAGATACCa > 1:378141/1‑67 (MQ=255) ccGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGCAAACACTTTCGGATGCGCCAGATACCa > 1:1321556/1‑67 (MQ=255) ccGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGCAAACACTTTCGGATGCGCCAGATACCa > 1:981580/1‑67 (MQ=255) ccGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGCAAACACTTTCGGATGCGCCAGATACCa > 1:1814554/1‑67 (MQ=255) ccGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGCAAACACTTTCGGATGCGCCAGATACCa > 1:1228554/1‑67 (MQ=255) ccGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGCAAACACTTTCGGATGCGCCAGATACCa > 1:2080341/1‑67 (MQ=255) ccGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGCAAACACTTTCGGATGCGCCAGATACCa > 1:2068548/1‑67 (MQ=255) gATAACGGCCGCCACGGCAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAAt < 1:977938/66‑1 (MQ=255) gATAACGGCCGCCACGGCAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAAt < 1:1848953/66‑1 (MQ=255) gATAACGGCCGCCACGGCAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAAt < 1:1465097/66‑1 (MQ=255) aTAACGGCCGCCACGGCAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAAt < 1:2005113/65‑1 (MQ=255) tAACGGCCGCCACGGTAAACACTTTCTGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGt > 1:112498/1‑67 (MQ=255) tAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATgg > 1:1922585/1‑66 (MQ=255) tAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGt > 1:665563/1‑67 (MQ=255) tAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGt > 1:1656402/1‑67 (MQ=255) tAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGt > 1:584742/1‑67 (MQ=255) tAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGt > 1:514782/1‑67 (MQ=255) tAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGt > 1:472202/1‑67 (MQ=255) tAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGt > 1:1152794/1‑67 (MQ=255) tAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGt > 1:882745/1‑67 (MQ=255) tAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGt > 1:1163582/1‑67 (MQ=255) tAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGt > 1:1170552/1‑67 (MQ=255) tAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGt > 1:1022188/1‑67 (MQ=255) tAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGt > 1:927606/1‑67 (MQ=255) tAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGt > 1:330342/1‑67 (MQ=255) tAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGt > 1:29510/1‑67 (MQ=255) tAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGt > 1:1401104/1‑67 (MQ=255) tAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGt > 1:1773977/1‑67 (MQ=255) | CCGCCGAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGT > minE/2948060‑2948174 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |