| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 60,568 | G→A | 43.1% | R125R (CGC→CGT) | hepA ← | RNA polymerase‑associated helicase protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 60,568 | 0 | G | A | 43.1% | 25.8 / 62.1 | 51 | R125R (CGC→CGT) | hepA | RNA polymerase‑associated helicase protein |
| Reads supporting (aligned to +/- strand): ref base G (17/12); new base A (8/14); total (25/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.60e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCAGGCCGCTGTACGGCATACGGAACTGTTCGCTGGAATATTTACGCGCGCGATAACGCAGCGCAAAGCGGTCCATACGGTCAATCTGCCCGGCAAACAGACGGTCCTGCGGTTTGCTGAACACCAGTTTGCTATCAA > minE/60501‑60638 | gcAGGCCGCTGTACGGCATACGGAACTGTTCGCTGGAATATTTACGCGCGCGATAACGCAGCGCAAAGCgg < 1:1837831/71‑1 (MQ=255) tACGGCATACGGAACTGTTCGCTGGAATATTTACGCGCGCGATAACGCAGCGCAAAGCGGTCCATa < 1:1817016/66‑1 (MQ=255) tACGGCATACGGAACTGTT‑‑CTGGAATATTTACGCGCGCGATAACGCAGCGCAAAGCGGTCCATa < 1:4051416/64‑1 (MQ=255) aCGGCATACGGAACTGTTCGCTGGAATATTTACGCGCGCGATAACGCAGCGCAAAGCGGTCCATACGGTc > 1:823181/1‑70 (MQ=255) aCGGCATACGGAACTGTTCGCTGGAATATTTACGCGCGCGATAACGCAGCGCAAAGCGGTCCATACGGTc > 1:3307323/1‑70 (MQ=255) aCGGCATACGGAACTGTTCGCTGGAATATTTACGCGCGCGATAACGCAGCGCAAAGCGGTCCATACGGTc > 1:2260752/1‑70 (MQ=255) aCGGCATACGGAACTGTTCGCTGGAATATTTACGCGCGCGATAACGCAGCGCAAAGCGGTCCATACGGTc > 1:1189538/1‑70 (MQ=255) cGGCATACGGAACTGTTCGCTGGAATATTTACGCGCGCGATAACGCAGCGCAAAGCGGTCCATACGGTCa < 1:485460/70‑1 (MQ=255) cGGCATACGGAACTGTTCGCTGGAATATTTACGCGCGCGATAACGCAGCGCAAAGCGGTCCATACGGTCa < 1:3290342/70‑1 (MQ=255) cGGCATACGGAACTGTTCGCTGGAATATTTACGCGCGCGATAACGCAGCGCAAAGCGGTCCATACGGTCa < 1:1993761/70‑1 (MQ=255) ggCATACGGAACTGTTCGCTGGAATATTTACGCGCGCGATAACGCAGCGCAAAACGGTCCATACGGTCAAt < 1:4064867/71‑1 (MQ=255) aTACGGAACTGTTCGCTGGAATATTTACGCGCGCGATAACGCAGCGCAAAACgg < 1:412212/54‑1 (MQ=255) cGGAACTGTTCGCTGGAATATTTACGCGCGCGATAACGCAGCGCAAAGCGGTCCATACGGTCAATCTGccc > 1:1346844/1‑71 (MQ=255) cGGAACTGTTCGCTGGAATATTTACGCGCGCGATAACGCAGCGCAAAGCGGTCCATACGGTCAATCTGccc > 1:672926/1‑71 (MQ=255) cGGAACTGTTCGCTGGAATATTTACGCGCGCGATAACGCAGCGCAAAACGGTCCATACGGTCAATCTGccc > 1:1871186/1‑71 (MQ=255) cGGAACTGTTCGCTGGAATATTTACGCGCGCGATAACGCAGCGCAAAACGGTCCATACGGTCAATCTGccc > 1:2175627/1‑71 (MQ=255) cGGAACTGTTCGCTGGAATATTTACGCGCGCGATAACGCAGCGCAAAACGGTCCATACGGTCAATCTGccc > 1:462705/1‑71 (MQ=255) gAACTGTTCGCTGGAATATTTACGCGCGCGATAACGCAGCGCAAAGCGGTCCATACGGTCAATCTGCCCgg > 1:190824/1‑71 (MQ=255) gAACTGTTCGCTGGAATATTTACGCGCGCGATAACGAAGCGCAAAACGGTCCATa < 1:2598813/55‑1 (MQ=255) aCTGTTCGCTGGAATATTTACGCGCGCGATAACGCAGCGCAAAGCGGTCCATACGGTCAATCTGCCCGGCa < 1:1702231/71‑1 (MQ=255) aCTGTTCGCTGGAATATTTACGCGCGCGATAACGCAGCGCAAAACGGTCCATACGGTCAATCTGCCCGGCa < 1:1148324/71‑1 (MQ=255) aCTGTTCGCTGGAATATTTACGCGCGCGATAACGCAGCGCAAAACGGTCCATACGGTCAATCTGCCCGGCa < 1:183316/71‑1 (MQ=255) aCTGTTCGCTGGAATATTTACGCGCGCGATAACGCAGCGCAAAACGGTCCATACGGTCAATCTGCCCGGCa < 1:2948651/71‑1 (MQ=255) aCTGTTCGCTGGAATATTTACGCGCGCGATAACGCAGCGCAAAACGGTCCATACGGTCAATCTGCCCGGCa < 1:795378/71‑1 (MQ=255) tGTTCGCTGGAATATTTACGCGCGCGATAACGCAGCGCAAAGCGGTCCATACGGTCAATCTGCCCGGCaaa > 1:1567812/1‑71 (MQ=255) tGTTCGCTGGAATATTTACGCGCGCGATAACGCAGCGCAAAGCGGTCCATACGGTCAATCTGCCCGGCaaa > 1:2206139/1‑71 (MQ=255) tGTTCGCTGGAATATTTACGCGCGCGATAACGCAGCGCAAAGCGGTCCATACGGTCAATCTGCCCGGCaaa > 1:1494109/1‑71 (MQ=255) gTTCGCTGGAATATTTACGCGCGCGATAACGCAGCGCAAAGCGGTCCATACGGTCAAt > 1:1997040/1‑58 (MQ=255) gTTCGCTGGAATATTTACGCGCGCGATAACGCAGCGCAAAACGGTCCATACGGTCAATCTGCCCGGCaaa > 1:4220589/1‑70 (MQ=255) gTTCGCTGGAATATTTACGCGCGCGATAACGCAGCGCAAAAAGGTCCATACGGTCAATCTGCCCGGCaaa > 1:375968/1‑70 (MQ=255) gTTCGCTGAAATATTTACGCGCGCGATAACGCAGCGCAAAGCGGTCCATACGGTCAAt > 1:2123092/1‑58 (MQ=255) ttCGCTGGAATATTTACGCGCGCGATAACGCAGCGCAAAGCGGTCCATACGGTCAATCTGCCCGGCaaa < 1:2314138/69‑1 (MQ=255) ttCGCTGGAATATTTACGCGCGCGATAACGCAGCGCAAAGCGGTCCATACGGTCAATCTGCCCGGCaaa < 1:1165828/69‑1 (MQ=255) ttCGCTGGAATATTTACGCGCGCGATAACGCAGCGCAAAACGGTCCATACGGTCAATCTGCCCGGCAAACa < 1:2113698/71‑1 (MQ=255) tCGCTGGAATATTTACGCGCGCGATAACGCAGCGCAAAACGGTCCATACGGTCAATCTGCCCGGCAAACAg > 1:1165335/1‑71 (MQ=255) ggAATATTTACGCGCGCGATAACGCAGCGCAAAACGGTCCATACGGTCAAt > 1:3782147/1‑51 (MQ=255) gAATATTTACGCGCGCGATAACGCAGCGCAAAACgg < 1:3544628/36‑1 (MQ=255) aaTATTTACGCGCGCGATAACGCAGCGCAAAGCGGTCCATACGGTCAATCTGCCCGGCAAACAGACGGTcc < 1:2874476/71‑1 (MQ=255) atatTTACGCGCGCGATAACGCAGCGCAAAGCGGTCCATACGGTCAATCTGCCCGGCAAACAGACGGTcc < 1:3045068/70‑1 (MQ=255) atatTTACGCGCGCGATAACGCAGCGCAAAGCGGTCCATACGGTCAATCTGCCCGGCAAACAGACGGTcat > 1:93995/1‑69 (MQ=255) atatTTACGCGCGCGATAACGCAGCGCAAAGCGGTCCATACGGTCAATCTGCCCGGCAAACAGACGGTCCt > 1:2761254/1‑71 (MQ=255) atatTTACGCGCGCGATAACGCAGCGCAAAGCGGTCCATACGGTCAATCTGCCCGGCAAACAGACGGTCCt > 1:2684015/1‑71 (MQ=255) atatTTACGCGCGCGATAACGCAGCGCAAAGCGGTCCATACGGTCAATCTGCCCGGCAAACAGACGGTCCt > 1:1209822/1‑71 (MQ=255) tatTTACGCGCGCGATAACGCAGCGCAAAACGGTCCATACGGTCAATCTGCCCGGCAAACAGACGGTCCTg < 1:3159682/71‑1 (MQ=255) tACGCGCGCGATAACGCAGCGCAAAGCGGTCCATACGGTCa > 1:2702437/1‑41 (MQ=255) cgcgcgATAACGCAGCGCAAAGCGGTCCATACGGTCAATCTGCCCGGCAAACAGACGGTCCTGCGGTTTGc < 1:3786494/71‑1 (MQ=255) gATAACGCAGCGCAAAACGGTCCATACGGTCAATCTGCCCGGCAAACAGACGGTCCTGCgg < 1:2237566/61‑1 (MQ=255) gATAACGCAGCGCAAAACGGTCCATACGGTCAATCTGCCCGGCAAACAGACGGTCCTGCGGTTTGCTg < 1:3983988/68‑1 (MQ=255) gATAACGCAGCGCAAAACGGTCCATACGGTCAATCTGCCCGGCAAACAGACGGTCCTGCGGTTTGCTGAac < 1:557407/71‑1 (MQ=255) gATAACGCAGCGCAAAACGGTCCATACGGGCAATCTGCCCGGCAAACAGACGGTCCTGCGGTTTGCTGa < 1:355604/69‑1 (MQ=255) tAACGCAGCGCAAAACGGTCCATACGGTCAATCTGCCCGGCAAACAGACGGTCCTGCGGTTTGCTGAaca > 1:3743280/1‑70 (MQ=255) gCGGTCCATACGGTCAATCTGCCCGGCAAACAGACGGTCCTGCGGTTTGCTGAACACCAGTTTGCTATCaa < 1:2739569/71‑1 (MQ=255) | GCAGGCCGCTGTACGGCATACGGAACTGTTCGCTGGAATATTTACGCGCGCGATAACGCAGCGCAAAGCGGTCCATACGGTCAATCTGCCCGGCAAACAGACGGTCCTGCGGTTTGCTGAACACCAGTTTGCTATCAA > minE/60501‑60638 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |