| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 249,414 | A→C | 12.6% | intergenic (+35/‑41) | brnQ → / → proY | predicted branched chain amino acid transporter/predicted cryptic proline transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 249,414 | 0 | A | C | 12.6% | 66.5 / 3.1 | 31 | intergenic (+35/‑41) | brnQ/proY | predicted branched chain amino acid transporter/predicted cryptic proline transporter |
| Reads supporting (aligned to +/- strand): ref base A (10/17); new base C (2/2); total (12/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.30e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGG > minE/249348‑249484 | cAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGcc < 1:3687704/71‑1 (MQ=255) gtcAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTTTTTTAACCACGGGGCATCGCAGCCCCGTg < 1:4007120/70‑1 (MQ=25) gtcAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCATCGCAGCCCCGTg < 1:1648202/70‑1 (MQ=37) aGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGtt < 1:1055242/71‑1 (MQ=255) aGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGtt < 1:1235186/71‑1 (MQ=255) aGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGtt < 1:1421521/71‑1 (MQ=255) aGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGtt < 1:480523/71‑1 (MQ=255) gcgcTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGtt < 1:102235/70‑1 (MQ=255) cTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGtt < 1:1556641/67‑1 (MQ=255) aCTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGt > 1:1155508/1‑71 (MQ=255) cTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGtt < 1:800536/63‑1 (MQ=255) cTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGtt < 1:2222894/63‑1 (MQ=255) aaTCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGtttttt > 1:3737439/1‑52 (MQ=255) aaTCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAgg < 1:3641500/71‑1 (MQ=255) aaTCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAg > 1:2257211/1‑70 (MQ=255) aaTCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAg > 1:3188291/1‑70 (MQ=255) aaTCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAg > 1:1704588/1‑70 (MQ=255) aaTCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGAAGGGTTAg > 1:1709453/1‑70 (MQ=255) cACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAAt > 1:4063729/1‑71 (MQ=255) cTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTg < 1:758387/71‑1 (MQ=255) aaCATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGa < 1:806386/69‑1 (MQ=255) aCATTTGTTTTAACCACGGGGCTGCGCTGCCCCGTGGTTTTTTATtgtg > 1:596464/1‑49 (MQ=255) aCATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGttt > 1:3677541/1‑40 (MQ=255) tttGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGaaa < 1:3603529/71‑1 (MQ=255) ttGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAg > 1:3507603/1‑71 (MQ=255) ttGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAg > 1:1826248/1‑71 (MQ=255) tGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAg < 1:562965/70‑1 (MQ=255) gTTTTAACCACGGGGCTGCGCTGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGaa > 1:3526216/1‑58 (MQ=255) aCGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGa < 1:1651436/53‑1 (MQ=255) gggCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAAtt < 1:803254/48‑1 (MQ=255) cTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGc < 1:4249942/71‑1 (MQ=255) aTGCCCCGTGGTTTTTTATTGTGTTGATGGTTTAgg < 1:2446801/36‑1 (MQ=255) aTGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAgg < 1:2851897/36‑1 (MQ=255) aTGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAgg < 1:1933706/36‑1 (MQ=255) aTGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAg < 1:607190/59‑1 (MQ=255) aTGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTggg > 1:2024805/1‑71 (MQ=255) | CAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGG > minE/249348‑249484 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |