| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,065,304 | A→C | 7.6% | E515D (GAA→GAC) | rpoD → | RNA polymerase, sigma 70 (sigma D) factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,065,304 | 0 | A | C | 7.6% | 253.6 / 20.5 | 106 | E515D (GAA→GAC) | rpoD | RNA polymerase, sigma 70 (sigma D) factor |
| Reads supporting (aligned to +/- strand): ref base A (63/35); new base C (4/4); total (67/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.62e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.57e-01 | |||||||||||
Only 100 of 110 total aligned reads displayed.
ACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCG > minE/2065234‑2065371 | aCAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGaa < 1:2755709/71‑1 (MQ=255)aCAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGaa < 1:1880114/71‑1 (MQ=255)aCAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGaa < 1:3557579/71‑1 (MQ=255)aCAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGaa < 1:562301/71‑1 (MQ=255) aGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGAt < 1:722895/71‑1 (MQ=255) tcagCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCg > 1:1060041/4‑71 (MQ=255) tCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCg > 1:1088184/1‑71 (MQ=255) tCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCg > 1:2728218/1‑71 (MQ=255) tCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCg > 1:2300954/1‑71 (MQ=255) tCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCg > 1:2656283/1‑71 (MQ=255) aaaGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCt < 1:2307002/71‑1 (MQ=255) aaaGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCt < 1:218585/71‑1 (MQ=255) tGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTgggg < 1:794942/71‑1 (MQ=255) atGAAGATTGCGAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGACGACGACGATTCGCATCTGGGCGa < 1:2734669/70‑1 (MQ=255) atGAAGATTGCGAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGACGACGACGATTCGCATCTGGGCGa < 1:539733/70‑1 (MQ=255) gAAGATTGCGAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGACGACGACGATTCGCATCTGGGCGa < 1:1103652/69‑1 (MQ=255) gATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCa > 1:1906674/1‑71 (MQ=255) tCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCt > 1:188129/1‑58 (MQ=255) cGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATc > 1:4160679/1‑70 (MQ=255) cGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATc > 1:1376201/1‑70 (MQ=255) cGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCT‑GGGGATTTCATc > 1:2489449/1‑69 (MQ=255) gCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCg < 1:2138166/70‑1 (MQ=255) ccAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAg < 1:258792/71‑1 (MQ=255) aaaGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGa < 1:4061185/71‑1 (MQ=255) aaaGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGa < 1:187460/71‑1 (MQ=255) aaaGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGa < 1:597021/71‑1 (MQ=255) aaaGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGa < 1:2056829/71‑1 (MQ=255) aaGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTg < 1:1005285/53‑1 (MQ=255) aaGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTg < 1:1843075/53‑1 (MQ=255) aaGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGa > 1:1766298/1‑70 (MQ=255) aaGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGa > 1:4015882/1‑70 (MQ=255) aaGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGAt < 1:206824/71‑1 (MQ=255) aaGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGAt < 1:2413665/71‑1 (MQ=255) gagCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTggg > 1:3158147/1‑53 (MQ=255) gagCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAg > 1:2325880/1‑66 (MQ=255) gagCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAg > 1:2749768/1‑66 (MQ=255) gagCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAg > 1:2764132/1‑66 (MQ=255) gagCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAg > 1:1900769/1‑66 (MQ=255) gagCCAATCTCCATGGAAACGCCGATCGGTGACGACGACGATTCGCATCTGGGCGATTTTATCGAGGa > 1:1687634/1‑68 (MQ=255) gagCCAATCTCCATGGAAACGCCGATCGGTGACGACGACGATTCGCATCTGGGCGATTTTATCGAGGa > 1:4238216/1‑68 (MQ=255) gagCCAATCTCCATGGAAACGCCGATCGGTGACGACGACGATTCGCATCTGGGCGATTTTATCGAGGa > 1:3281185/1‑68 (MQ=255) gagCCAATCTCCATGGAAACGCCGATCGGTGACGACGACGATTCGCATCTGGGCGATTTTATCGAGGa > 1:709266/1‑68 (MQ=255) ccAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGa > 1:2518560/1‑65 (MQ=255) ccAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGa > 1:551090/1‑65 (MQ=255) ccAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGa > 1:1928785/1‑65 (MQ=255) aTCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATc < 1:2501722/57‑1 (MQ=255) tctcCATGGAAACGCCGATCGGTGATGATGAAGAt < 1:766894/35‑1 (MQ=255) tctcCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTggggg < 1:606987/48‑1 (MQ=255) tctcCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGa > 1:1569285/1‑58 (MQ=255) ctcCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCAccc > 1:1045689/1‑68 (MQ=255) ctcCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTc > 1:1165973/1‑70 (MQ=255) tcCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTc < 1:940158/51‑1 (MQ=255) tcCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAg > 1:775836/1‑57 (MQ=255) tcCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAg > 1:2152072/1‑57 (MQ=255) tcCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAg > 1:2878610/1‑57 (MQ=255) tcCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTc > 1:1681168/1‑69 (MQ=255) tcCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTc > 1:1731627/1‑69 (MQ=255) cATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTc > 1:2465121/1‑67 (MQ=255) cATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTc > 1:1638111/1‑67 (MQ=255) cATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTc > 1:2182538/1‑67 (MQ=255) cATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAg < 1:2795790/70‑1 (MQ=255) cATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGc > 1:687364/1‑71 (MQ=255) aTGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCg < 1:3701678/52‑1 (MQ=255) aTGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCt < 1:2922867/71‑1 (MQ=255) aTGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCt < 1:345118/71‑1 (MQ=255) aTGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCt < 1:3657052/71‑1 (MQ=255) ggAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGa > 1:2539986/1‑54 (MQ=255) ggAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGa > 1:2218340/1‑54 (MQ=255) ggAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGa > 1:2832599/1‑54 (MQ=255) ggAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGa > 1:3523336/1‑54 (MQ=255) ggAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGa > 1:1751771/1‑54 (MQ=255) gAAACGCCGATCGGTGATGATGAAGATTCGCATCt > 1:1690168/1‑35 (MQ=255) gAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGcc > 1:2476499/1‑71 (MQ=255) gAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGc > 1:1920338/1‑70 (MQ=255) aaCGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGc < 1:2930853/71‑1 (MQ=255) aCGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCAccc < 1:1770446/58‑1 (MQ=255) cGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTg < 1:692985/71‑1 (MQ=255) gCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAg < 1:2488736/46‑1 (MQ=255) gCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGa > 1:131819/1‑48 (MQ=255) gCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGa > 1:2837606/1‑48 (MQ=255) cGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGa > 1:187037/1‑46 (MQ=255) cGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGa > 1:2610645/1‑46 (MQ=255) cGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATaccac > 1:1242852/1‑52 (MQ=255) cGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGAt > 1:807155/1‑71 (MQ=255) tCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGa > 1:3348527/1‑40 (MQ=255) tCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGa > 1:3178993/1‑40 (MQ=255) tCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGa > 1:2779735/1‑40 (MQ=255) tCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGa > 1:3635406/1‑40 (MQ=255) tCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGa > 1:936429/1‑40 (MQ=255) tCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGa > 1:3741131/1‑40 (MQ=255) cGGTGATGATGAAGATTCGCTTCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTg > 1:391621/1‑71 (MQ=255) cGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTg > 1:1543789/1‑71 (MQ=255) tgatgatgaAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTg > 1:4172249/1‑61 (MQ=255) atgatgaAGATTCGCATCTGGGGGATTTCATCGAGGa > 1:3087682/1‑37 (MQ=255) atgatgaAGATTCGCATCTGGGGGATTTCATCGAGGa > 1:1852795/1‑37 (MQ=255) atgatgaAGATTCGCATCTGGGGGATTTCATCGAGGATacc < 1:3098027/41‑1 (MQ=255) atgatgaAGATTCGCATCTGGGGGATTTCATCGAGGATacc < 1:3759643/41‑1 (MQ=255) atgatgaAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGc < 1:4213970/57‑1 (MQ=255) atgaAGATTCGCATCTGGGGGATTTCATCGAGGATACCAACCTCGAGCTGCCGCTGGATTATGCGcccacc > 1:1085443/1‑71 (MQ=255) gaAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCg > 1:2760360/1‑70 (MQ=255) | ACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCG > minE/2065234‑2065371 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |