| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,124,793 | C→T | 6.4% | Q70Q (CAG→CAA) | yhbP ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,124,793 | 0 | C | T | 6.4% | 202.5 / 3.7 | 62 | Q70Q (CAG→CAA) | yhbP | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (35/23); new base T (2/2); total (37/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTGCACACCGCGAATTAACGCTACCGTTTTCGGCTGACCGTTTACCGTTCCGGCAACCGCAGCCTGCGGCCCACTCATCTGCGCGTGGCGCGTTTTTTCT > minE/2124725‑2124859 | tCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTGCACACCGCGAATTAACGCTACCGTTTTCGGCt > 1:470578/1‑70 (MQ=255)tCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTGCACACCGCGAATTAACGCTACCGTTTTCGGCt > 1:519810/1‑70 (MQ=255) ccTTCCAGCCTGCGGATCTCACCTTTAAACTGCACACCGCGAATTAACGCTACCGTTTTCGGCTGACCGtt < 1:1085927/71‑1 (MQ=255) ccTTCCAGCCTGCGGATCTCACCTTTAAACTGCACACCGCGAATTAACGCTACCGTTTTCGGCTGACCGtt < 1:651874/71‑1 (MQ=255) ccTTCCAGCCTGCGGATCTCACCTTTAAACTGCACACCGCGAATTAACGCTACCGTTTTCGGCTGACCGtt < 1:665424/71‑1 (MQ=255) cTTCCAGCCTGCGGATCTCACCTTTAAACTGCACACCGCGAATTAACGCTACCGTTTTCGGTTGACCGtt > 1:1278609/1‑70 (MQ=255) cTTCCAGCCTGCGGATCTCACCTTTAAACTGCACACCGCGAATTAACGCTACCGTTTTCGGCTGACCGtt > 1:3772731/1‑70 (MQ=255) cTTCCAGCCTGCGGATCTCACCTTTAAACTGCACACCGCGAATTAACGCTACCGTTTTCGGCTGACCGtt > 1:2393027/1‑70 (MQ=255) cTTCCAGCCTGCGGATCTCACCTTTAAACTGCACACCGCGAATTAACGCTACCGTTTTCGGCTGACCGt > 1:2271971/1‑69 (MQ=255) ccAGCCTGCGGATCTCACCTTTAAACTGCACACCGCGAATTA‑‑GCTACCGTTTTCGGTTGACCGTTTACCGt > 1:913345/1‑71 (MQ=255) cGGATCTCACCTTTAAACTGCACACCGCGAATTAACGCTACCGTTTTCGGCTGACCGTTTACCGTTCCgg > 1:1233423/1‑70 (MQ=255) ggATCTCACCTTTAAACTGCACACCGCGAATTAACGCTACCGTTTTCGGCTGACCGTTTACCGTTCCGGc > 1:1386188/1‑70 (MQ=255) ggATCTCACCTTTAAACTGCACACCGCGAATTAACGCTACCGTTTTCGGCTGACCGTTTACCGTTCCGGc > 1:519489/1‑70 (MQ=255) ggATCTCACCTTTAAACTGCACACCGCGAATTAACGCTACCGTTTTCGGCTGACCGTTTACCGTTCCGGCa < 1:3788212/71‑1 (MQ=255) aTCTCACCTTTAAACTGCACACCGCGAATTAACGCTACCGTTTTCGGCTGACCGt < 1:4245434/55‑1 (MQ=255) aTCTCACCTTTAAACTGCACACCGCGAATTAACGCTACCGTTTTCGGCTGACCGt < 1:1999298/55‑1 (MQ=255) aTCTCACCTTTAAACTGCACACCGCGAATTAACGCTACCGTTTTCGGCTGACCGt < 1:425798/55‑1 (MQ=255) aTCTCACCTTTAAACTGCACACCGCGAATTAACGCTACCGTTTTCGGCTGACCGt < 1:918424/55‑1 (MQ=255) tctcACCTTTAAACTGCACACCGCGAATTAACGCTACCGTTTTCGGCTGACCGTTTACCGTTCCGGCAAcc > 1:652232/1‑71 (MQ=255) tctcACCTTTAAACTGCACACCGCGAATTAACGCTACCGTTTTCGGCTGACCGTTTACCGTTCCGGCAAcc > 1:1275830/1‑71 (MQ=255) ctcACCTTTAAACTGCACACCGCGAATTAACGCTACCGTTTTCGGCTGACCGTTTACCGTTCCGGCAACCg > 1:2165237/1‑71 (MQ=255) ctcACCTTTAAACTGCACACCGCGAATTAACGCTACCGTTTTCGGCTGACCGTTTACCGTTCCGGCAACCg > 1:2978978/1‑71 (MQ=255) cACCTTTAAACTGCACACCGCGAATTAACGCTACCGTTTTCGGCTGACCGTTTACCGTTCCGGCAACCg > 1:731510/1‑69 (MQ=255) cACCTTTAAACTGCACACCGCGAATTAACGCTACCGTTTTCGGCTGACCGTTTACCGTTCCGGCAACCg > 1:301996/1‑69 (MQ=255) aCCTTTAAACTGCACACCGCGAATTAACGCTACCGTTTTCGTCTGACCGtt > 1:688054/1‑51 (MQ=255) aCCTTTAAACTGCACACCGCGAATTAACGCTACCGTTTTCGGTTGACCGTTTACCGTTCCGGCAACCGCAg < 1:2547781/71‑1 (MQ=255) aCCTTTAAACTGCACACCGCGAATTAACGCTACCGTTTTCGGTTGACCGTTTACCGTTCCGGCAACCGCAg < 1:378144/71‑1 (MQ=255) aCCTTTAAACTGCACACCGCGAATTAACGCTACCGTTTTCGGCTGACCGtt > 1:465674/1‑51 (MQ=255) aCCTTTAAACTGCACACCGCGAATTAACGCTACCGTTTTCGGCTGACCGtt > 1:1114873/1‑51 (MQ=255) aCCTTTAAACTGCACACCGCGAATTAACGCTACCGTTTTCGGCTGACCGt > 1:2819316/1‑50 (MQ=255) aCCTTTAAACTGCACACCGCGAATTAACGCTACCGTTTTCGGCTGACCGt > 1:3299760/1‑50 (MQ=255) aCCTTTAAACTGCACACCGCGAATTAACGCTACCGTTTTCGGCTGACCGt > 1:3848130/1‑50 (MQ=255) aCCTTTAAACTGCACACCGCGAATTAACGCTACCGTTTTCGGCTGACCGt > 1:1678486/1‑50 (MQ=255) aCCTTTAAACTGCACACCGCGAATTAACGCTACCGTTTTCGGCTGACCGTTTACCGTTCCGGCa < 1:1971442/64‑1 (MQ=255) tAAACTGCACACCGCGAATTAACGCTACCGTTTTCGGCTGACCGTTTACCGTTCCGGCaa > 1:4249625/1‑60 (MQ=255) tAAACTGCACACCGCGAATTAACGCTACCGTTTTCGGCTGACCGTTTACCGTTCCGGCaa > 1:1059211/1‑60 (MQ=255) cacCGCGAATTAACGCTACCGTTTTCGGCTGACCGTTTACCGTTCCGGCAACCGCAGCCTGCGGCCCACTc < 1:3484463/71‑1 (MQ=255) ccGCGAATTAACGCTACCGTTTTCGGCTGACCGTTTACCGTTCCGGc < 1:3498283/47‑1 (MQ=255) cgAATTAACGCTACCGTTTTCGGCTGACCGTTTACCGTTCCGGCAACCGCAGCCTGCGGCCCACTCATCTg < 1:1177067/71‑1 (MQ=255) cgAATTAACGCTACCGTTTTCGGCTGACCGTTTACCGTTCCGGCAACCGCAGCCTGCGGCCCACTCATCTg > 1:3022791/1‑71 (MQ=255) gAATTAACGCTACCGTTTTCGGCTGACCGTTTACCGTTCCGGCAACCGCAGCCTGCGGCCCACTCATCTgc < 1:3752954/71‑1 (MQ=255) aTTAACGCTACCGTTTTCGGCTGACCGTTTACCGTTCCGGCAACCGCAGCCTGCGGCCCACTc < 1:4199154/63‑1 (MQ=255) aTTAACGCTACCGTTTTCGGCTGACCGTTTACCGTTCCGGCAACCGCAGCCTGCGGCCCACTCATCTgcgc < 1:1339700/71‑1 (MQ=255) aTTAACGCTACCGTTTTCGGCTGACCGTTTACCGTTCCGGCAACCGCAGCCTGCGGCCCACTCATCTgcgc < 1:452558/71‑1 (MQ=255) ttAACGCTACCGTTTTCGGCTGACCGTTTACCGTTCCGGc < 1:3647475/40‑1 (MQ=255) aaCGCTACCGTTTTCGGCTGACCGTTTACCGTTCCGGCaa > 1:1214297/1‑40 (MQ=255) aaCGCTACCGTTTTCGGCTGACCGTTTACCGTTCCGGCaa > 1:2139687/1‑40 (MQ=255) gCTACCGTTTTCGGCTGACCGTTTACCGTTCCGGCACCCGAAGCCTGCGGCCCACTCATCTGCGCGTGgcg > 1:3865806/1‑71 (MQ=255) gCTACCGTTTTCGGCTGACCGTTTACCGTTCCGGCAACCGCAGCCTGCGGCCCACTCATCTGCGCGTGgcg > 1:3138212/1‑71 (MQ=255) gCTACCGTTTTCGGCTGACCGTTTACCGTTCCGGCAACCGAAGCCTGCGGCCCACTCATCTGCGCGTGgcg > 1:3861132/1‑71 (MQ=255) ccGTTTTCGGCTGACCGTTTACCGTTCCGGCAACCGCAGCCTGCGGCCCa > 1:334751/1‑50 (MQ=255) ccGTTTTCGGCTGACCGTTTACCGTTCCGGCAACCGCAGCCTGCGGCCCa > 1:1863859/1‑50 (MQ=255) ccGTTTTCGGCTGACCGTTTACCGTTCCGGCAACCGCAGCCTGCGGCCCa > 1:4061844/1‑50 (MQ=255) ccGTTTTCGGCTGACCGTTTACCGTTCCGGCAACCGCAGCCTGCGGCCCACTCATCTGCGCGTGGCGCGt < 1:4250802/70‑1 (MQ=255) gTTTTCGGCTGACCGTTTACCGTTCCGGCAACCGCAGCCTGCGGCCCACTCATCTGCGCGTGGCGCGtttt > 1:2942537/1‑71 (MQ=255) gTTTTCGGCTGACCGTTTACCGTTCCGGCAACCGCAGCCTGCGGCCCACTCATCTGCGCGTGGCGCGtttt < 1:2894605/71‑1 (MQ=255) gTTTTCGGCTGACCGTTTACCGTTCCGGCAACCGCAGCCTGCGGCCCACTCATCTGCGCGTGGCGCGtttt < 1:1401409/71‑1 (MQ=255) gTTTTCGGCTGACCGTTTACCGTTCCGGCAACCGCAGCCTGCGGCCCACTCATCTGCGCGTGGCGCGtttt > 1:3867549/1‑71 (MQ=255) ttCGGCTGACCGTTTACCGTTCCGGCAACCGCAGCCTGCGGCCCACTCATCTGCGCGTGGCGCGtttttt > 1:1787616/1‑70 (MQ=255) tCGGCTGACCGTTTACCGTTCCGGCAACCCCAGCCTGCGGCCCACTCATCTg < 1:326420/52‑1 (MQ=255) cGGCTGACCGTTTACCGTTCCGGCAACCGCAGCCTGCGGCCCACTCATCTGCGCGTGGCGCGTTTTttct < 1:771674/70‑1 (MQ=255) cGGCTGACCGTTTACCGTTCCGGCAACCGCAGCCTGCGGCCCACTCATCTGCGCGTGGCGCGTTTTttct < 1:96647/70‑1 (MQ=255) | TCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTGCACACCGCGAATTAACGCTACCGTTTTCGGCTGACCGTTTACCGTTCCGGCAACCGCAGCCTGCGGCCCACTCATCTGCGCGTGGCGCGTTTTTTCT > minE/2124725‑2124859 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |