| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,144,345 | G→A | 45.0% | D206N (GAT→AAT) | argG → | argininosuccinate synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,144,345 | 0 | G | A | 45.0% | 35.4 / 123.6 | 91 | D206N (GAT→AAT) | argG | argininosuccinate synthetase |
| Reads supporting (aligned to +/- strand): ref base G (20/30); new base A (17/24); total (37/54) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AATGTCTGTCGAAAAAGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAATTCTGGGAT > minE/2144281‑2144410 | aaTGTCTGTCGAAAAAGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGAATCTgg < 1:2671956/71‑1 (MQ=255) tctgtcGAAAAAGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATa < 1:3617213/71‑1 (MQ=255) tcGAAAAAGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTgg < 1:2603356/63‑1 (MQ=255) tcGAAAAAGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGAATCTgg < 1:2975273/63‑1 (MQ=255) tcGAAAAAGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGAATCTgg < 1:459278/63‑1 (MQ=255) tcGAAAAAGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGAATCTgg < 1:3809518/63‑1 (MQ=255) gAAAAAGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGaa > 1:2020302/1‑63 (MQ=255) aaaaaGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATc < 1:211703/57‑1 (MQ=255) aaaaaGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATc < 1:3759460/57‑1 (MQ=255) gCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGAATCTgg < 1:922399/55‑1 (MQ=255) tACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCa < 1:3520407/67‑1 (MQ=255) tACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCa < 1:539660/67‑1 (MQ=255) tACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCa < 1:158298/67‑1 (MQ=255) tCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGAATCTGGAATACCTCAACTCCAGCGTCa > 1:4065092/1‑70 (MQ=255) tCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGAATCTGGAATACCTCAACTCCAGCGTCa > 1:2349126/1‑70 (MQ=255) tCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGAATCTGGAATACCTCAACTCCAGCGTCa > 1:3036346/1‑70 (MQ=255) cacaGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGa < 1:2869574/42‑1 (MQ=255) cacaGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCaaa < 1:424499/70‑1 (MQ=255) cacaGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCaaa < 1:1210859/70‑1 (MQ=255) cacaGACTACAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGaa > 1:2342585/1‑49 (MQ=255) acaGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATa > 1:1464837/1‑50 (MQ=255) acaGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATAc > 1:1815530/1‑51 (MQ=255) acaGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGAATCTGGAATACCTCAACTCCAGCGTCAAAAt > 1:3469066/1‑71 (MQ=255) caGACTCCAACATGCTTGGTGCAACGCATGAAGCTAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATc < 1:610878/71‑1 (MQ=255) caGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATc < 1:3779486/71‑1 (MQ=255) aCTCCAACATGCTTGGTGCAACGCATGAAGCGAAGAATCTGGAATACCTCAACTCCAGCGTCaaa < 1:116041/65‑1 (MQ=255) tCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTgg > 1:2699081/1‑40 (MQ=255) tCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTgg > 1:2643810/1‑40 (MQ=255) tCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTgg > 1:980873/1‑40 (MQ=255) tCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTgg > 1:1177503/1‑40 (MQ=255) tCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTgg > 1:1279475/1‑40 (MQ=255) tCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATc < 1:843391/66‑1 (MQ=255) tCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATc < 1:2567271/66‑1 (MQ=255) tCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATc < 1:160864/66‑1 (MQ=255) tCCAACATGCTTGGTGCAACGCATGAAGCGAAGAATCTGGAATACCTCAACTCCAGCGTCaaaa > 1:2524624/1‑64 (MQ=255) tCCAACATGCTTGGTGCAACGCATGAAGCGAAGAATCTGGAATACCTCAACTCCAGCGTCaaaa > 1:2540427/1‑64 (MQ=255) tCCAACATGCTTGGTGCAACGCATGAAGCGAAGAATCTGGAATACCTCAACTCCAGCGTCaaaa > 1:2935372/1‑64 (MQ=255) tCCAACATGCTTGGTGCAACGCATGAAGCGAAGAATCTGGAATACCTCAACTCCAGCGTCAAAAt > 1:1109877/1‑65 (MQ=255) ccAACATGCTTGGTGCAACGCATGAAGCGAAGAATCTgg < 1:140316/39‑1 (MQ=255) ccAACATGCTTGGTGCAACGCATGAAGCGAAGAATCTgg < 1:3730138/39‑1 (MQ=255) ccAACATGCTTGGTGCAACGCATGAAGCGAAGAATCTgg < 1:358343/39‑1 (MQ=255) ccAACATGCTTGGTGCAACGCATGAAGCGAAGAATCTgg < 1:2759927/39‑1 (MQ=255) cAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAAcc > 1:123452/1‑71 (MQ=255) aaCATGCTTGGTGCAACGCATGAAGCGAAGAATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAAccc < 1:3319367/71‑1 (MQ=255) aCATGCTTGGTGCAACGCATGAAGCGAAGAATCTGGAATACCTCaa > 1:2670236/1‑46 (MQ=255) aTGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGAt < 1:505541/71‑1 (MQ=255) aTGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGAt < 1:1616168/71‑1 (MQ=255) tGCTTGGTGCAACGCATGAAGCGAAGAATCTGGAATa > 1:413737/1‑37 (MQ=255) tGCTTGGTGCAACGCATGAAGCGAAGAATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGAtt < 1:3639102/71‑1 (MQ=255) cTTGGTGCAACGCATGAAGCGAAGAATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTAt < 1:3135097/71‑1 (MQ=255) ttGGTGCAACGCATGAAGCGAAGAATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATg > 1:1360774/1‑71 (MQ=255) ttGGTGAAACGCATGAAGCGAAGAATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATg > 1:2854659/1‑71 (MQ=255) tGGTGCAACGCATGAAGCGAAGAATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATgg > 1:3176158/1‑71 (MQ=255) tGGTGCAACGCATGAAGCGAAGAATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATgg > 1:3248112/1‑71 (MQ=255) ggTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCg < 1:3281305/55‑1 (MQ=255) ggTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCg < 1:3670049/55‑1 (MQ=255) ggTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCg < 1:2208329/55‑1 (MQ=255) ggTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCg < 1:2651325/55‑1 (MQ=255) ggTGCAACGCATGAAGCGAAGGATCTAGAATACCTCAACTCCAGCGTCAAAATCg < 1:811956/55‑1 (MQ=255) ggTGCAACGCATGAAGCGAAGAATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGAtt > 1:3633810/1‑66 (MQ=255) ggTGCAACGCATGAAGCGAAGAATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATgg < 1:253528/70‑1 (MQ=255) ggTGCAACGCATGAAGCGAAGAATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATgg < 1:2348378/70‑1 (MQ=255) gatGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCg < 1:752698/53‑1 (MQ=255) gTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGc < 1:1472408/71‑1 (MQ=255) gTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGc < 1:1544375/71‑1 (MQ=255) tGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTAt < 1:4146528/66‑1 (MQ=255) tGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTAt < 1:3655767/66‑1 (MQ=255) tGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTAt < 1:1368956/66‑1 (MQ=255) tGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTAt < 1:3791085/66‑1 (MQ=255) tGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTAt < 1:387807/66‑1 (MQ=255) gCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGt > 1:862464/1‑71 (MQ=255) gCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGt > 1:4188067/1‑71 (MQ=255) aCGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTg > 1:1368665/1‑69 (MQ=255) aCGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGa > 1:293130/1‑70 (MQ=255) cATGAAGCGAAGAATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAAtt < 1:2684475/71‑1 (MQ=255) cATGAAGCGAAGAATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAAtt < 1:208133/71‑1 (MQ=255) cATGAAGCGAAGAATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAAtt < 1:2247840/71‑1 (MQ=255) cATGAAGCGAAGAATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAAtt < 1:622440/71‑1 (MQ=255) cATGAAGCGAAGAATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAAtt < 1:29189/71‑1 (MQ=255) tGAAGCGAAGAATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAATTCt < 1:1926860/71‑1 (MQ=255) tGAAGCGAAGAATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAATTCt < 1:4166984/71‑1 (MQ=255) aaGCGAAGAATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAAccc < 1:2299597/49‑1 (MQ=255) aaGCGAAGAATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAAccc < 1:1448471/49‑1 (MQ=255) gCGAAGAATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGa > 1:133168/1‑49 (MQ=255) cGAAGAATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAATTCTGGGAt > 1:3157783/1‑71 (MQ=255) gaagaaTCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGAt < 1:2124468/48‑1 (MQ=255) gaagaaTCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGAt < 1:495088/48‑1 (MQ=255) gaagaaTCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGAt < 1:2962342/48‑1 (MQ=255) gaagaaTCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGAt < 1:2698116/48‑1 (MQ=255) gaagaaTCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGAt < 1:1159370/48‑1 (MQ=255) gaagaaTCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGAt < 1:1836574/48‑1 (MQ=255) aaGGATCTGGAATACCTCAACTCCAGCGTCAAAAt > 1:1265812/1‑35 (MQ=255) aaGGATCTGGAATACCTCAACTCCAGCGTCAAAAt > 1:434778/1‑35 (MQ=255) aaGGATCTGGAATACCTCAACTCCAGCGTCAAAAt > 1:3647628/1‑35 (MQ=255) aaGGATCTGGAATACCTCAACTCCAGCGTCAAAAt > 1:3642615/1‑35 (MQ=255) aaGGATCTGGAATACCTCAACTCCAGCGTCAAAAt > 1:1167476/1‑35 (MQ=255) aaGGATCTGGAATACCTCAACTCCAGCGTCAAAAt > 1:2916938/1‑35 (MQ=255) | AATGTCTGTCGAAAAAGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAATTCTGGGAT > minE/2144281‑2144410 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |