Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,168,106 T→A 5.0% Y60N (TAT→AAT)  yrbK → conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,168,1060TA 5.0% 287.8 / 6.0 99Y60N (TAT→AAT) yrbKconserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (48/46);  new base A (3/2);  total (51/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCCCACCTATAAAAGCGAGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTACGCAGCCGGTA  >  minE/2168044‑2168174
                                                              |                                                                    
aTCCCACCTATAAAAGCGAGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCgatt                                                               <  1:2410259/70‑1 (MQ=255)
aTCCCACCTATAAAAGCGAGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCgatt                                                               <  1:2777089/70‑1 (MQ=255)
aTCCCACCTATAAAAGCGAGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCgatt                                                               <  1:3984918/70‑1 (MQ=255)
aTCCCACCTATAAAAGCGAGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCgatt                                                               <  1:3700301/70‑1 (MQ=255)
  cccACCTATAAAAGCGAGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCgattgat                                                            >  1:2093670/1‑71 (MQ=255)
  cccACCTATAAAAGCGAGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCgattgat                                                            >  1:2570456/1‑71 (MQ=255)
  cccACCTATAAAAGCGAGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCgattgat                                                            >  1:3642426/1‑71 (MQ=255)
     aCCTATAAAAGCGAGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGc                                                         >  1:997560/1‑71 (MQ=255)
         ataAAAGCGAGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCaa                                                     >  1:2797023/1‑71 (MQ=255)
          taAAAGCGAGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCaa                                                     >  1:2805253/1‑70 (MQ=255)
          taAAAGCGAGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCaa                                                     <  1:295165/70‑1 (MQ=255)
          taAAAGCGAGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCaa                                                     <  1:3747889/70‑1 (MQ=255)
          taAAAGCGAGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCa                                                      <  1:2462704/69‑1 (MQ=255)
            aaaGCGAGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAaca                                                   <  1:1684545/70‑1 (MQ=255)
                  aGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGaa                                            <  1:3826743/71‑1 (MQ=255)
                  aGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGaa                                            <  1:4138536/71‑1 (MQ=255)
                  aGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGaa                                            <  1:446865/71‑1 (MQ=255)
                  aGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGaa                                            <  1:2324060/71‑1 (MQ=255)
                    cATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCgattga                                                             >  1:1349472/1‑52 (MQ=255)
                    cATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCgattga                                                             >  1:744608/1‑52 (MQ=255)
                    cATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCgattga                                                             >  1:2283406/1‑52 (MQ=255)
                      tACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATc                                                                   <  1:1567761/44‑1 (MQ=255)
                       aCGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAAtatta                                       >  1:4007687/1‑71 (MQ=255)
                        cGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAAtattat                                      >  1:2814694/1‑71 (MQ=255)
                        cGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAAtattat                                      >  1:3499058/1‑71 (MQ=255)
                        cGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAAtattat                                      <  1:2619735/71‑1 (MQ=255)
                        cGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAAtattat                                      >  1:655616/1‑71 (MQ=255)
                        cGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAAtatta                                       >  1:688776/1‑70 (MQ=255)
                           acacGCTCGTCTATAACCCAGAAGGGGCACTAAGCAATCAATCGATAGCt                                                        <  1:3166054/50‑1 (MQ=25)
                           acacGCTCGTCTATAACCCAGAAGGGGCACTAAGCAATCAATCGATAGCt                                                        <  1:232524/50‑1 (MQ=25)
                             acGCTCGTCTATAACCCAGAAGGGGCACTAAGCTAt                                                                    >  1:1261478/1‑36 (MQ=255)
                             acGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAata                                          >  1:3499774/1‑62 (MQ=255)
                             acGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGa                                 <  1:3712488/71‑1 (MQ=255)
                             acGCTCGTCTATAACCCAGAAGGGGCACTAAGCAATCAATCGATAGCtta                                                      >  1:17325/1‑48 (MQ=21)
                             acGCTCGTCTATAACCCAGAAGGGGCACTAAGCAATCAATCGATAGCtta                                                      >  1:3082134/1‑48 (MQ=21)
                             acGCTCGTCTATAACCCAGAAGGGGCACTAAGCAATCAATCGATAGCtta                                                      >  1:1692159/1‑48 (MQ=21)
                               gCTCTTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAat                                           >  1:635203/1‑59 (MQ=255)
                               gCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCgattg                                                              <  1:2472736/40‑1 (MQ=255)
                               gCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCgattg                                                              <  1:456039/40‑1 (MQ=255)
                               gCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCgattg                                                              <  1:3669022/40‑1 (MQ=255)
                               gCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCgattg                                                              <  1:1474242/40‑1 (MQ=255)
                               gCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGAt                                >  1:2541305/1‑70 (MQ=255)
                                cTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAata                                          <  1:2222012/59‑1 (MQ=255)
                                cTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCa                              <  1:300955/71‑1 (MQ=255)
                                    tCTATAACCCAGATGGGGCAGTAAGCTATCTATTGATTGCTCAACACGTTGaa                                            <  1:1314353/53‑1 (MQ=255)
                                      tataACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAAtatta                                       >  1:3160071/1‑56 (MQ=255)
                                      tataACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAAtatta                                       >  1:3154849/1‑56 (MQ=255)
                                      tataACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAAtatta                                       >  1:3141869/1‑56 (MQ=255)
                                      tataACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAAtatta                                       >  1:3126148/1‑56 (MQ=255)
                                      tataACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAAtatta                                       >  1:3547120/1‑56 (MQ=255)
                                      tataACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAAtatta                                       >  1:890936/1‑56 (MQ=255)
                                       ataACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGtt                       >  1:4187689/1‑71 (MQ=255)
                                       ataACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGtt                       >  1:2752850/1‑71 (MQ=255)
                                         aaCCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATCCCGATCAGGCCGTTTc                     >  1:1346671/1‑71 (MQ=255)
                                          aCCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCg                    <  1:2908913/71‑1 (MQ=255)
                                           cccAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTCAATATTATTCCGATCAGGCCGTTTCg                    <  1:427989/70‑1 (MQ=255)
                                               gAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGtt               <  1:3100886/71‑1 (MQ=255)
                                                aaGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAg                             <  1:675807/56‑1 (MQ=255)
                                                aaGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAg                             <  1:1643106/56‑1 (MQ=255)
                                                aaGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAg                             <  1:3205267/56‑1 (MQ=255)
                                                 aGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAAtatta                                       >  1:295164/1‑45 (MQ=255)
                                                 aGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAAtatta                                       >  1:1427102/1‑45 (MQ=255)
                                                 aGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAAtatta                                       >  1:1006318/1‑45 (MQ=255)
                                                 aGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAAtatta                                       >  1:2901300/1‑45 (MQ=255)
                                                 aGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTa             <  1:1780166/71‑1 (MQ=255)
                                                  ggggCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGc                           >  1:3927442/1‑56 (MQ=255)
                                                  ggggCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGc                           >  1:394922/1‑56 (MQ=255)
                                                      cACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTc                     <  1:2121294/58‑1 (MQ=255)
                                                        cTAAGCTATCGATTGATTGCTCAACACGTTGAAtatt                                        >  1:3966166/1‑37 (MQ=255)
                                                        cTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTACGCa         <  1:3172701/68‑1 (MQ=255)
                                                        cTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTACGCAGcc      <  1:26190/71‑1 (MQ=255)
                                                        cTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTACGCAGcc      <  1:3742572/71‑1 (MQ=255)
                                                         tAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCg                                  <  1:1828210/42‑1 (MQ=255)
                                                         tAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCg                                  <  1:3141003/42‑1 (MQ=255)
                                                         tAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCg                                  <  1:1933912/42‑1 (MQ=255)
                                                         tAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTAc            <  1:1070935/64‑1 (MQ=255)
                                                         tAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTAc            <  1:301122/64‑1 (MQ=255)
                                                         tAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTAc            <  1:2737450/64‑1 (MQ=255)
                                                         tAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTAc            <  1:2713415/64‑1 (MQ=255)
                                                         tAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTAc            <  1:2660513/64‑1 (MQ=255)
                                                         tAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTACGCAGc       <  1:2417732/69‑1 (MQ=255)
                                                          aaGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGAt                                >  1:3277332/1‑43 (MQ=255)
                                                          aaGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGAt                                >  1:17617/1‑43 (MQ=255)
                                                          aaGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGAt                                >  1:974385/1‑43 (MQ=255)
                                                          aaGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGAt                                >  1:4169916/1‑43 (MQ=255)
                                                          aaGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGAt                                >  1:1291154/1‑43 (MQ=255)
                                                          aaGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTACGCAGCCgg    >  1:2274826/1‑71 (MQ=255)
                                                          aaGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTACGCAGCCgg    >  1:2462023/1‑71 (MQ=255)
                                                          aaGCTATCGATTGATTGCTCAACACGGTGAATATTATTCCGAt                                >  1:3949919/1‑43 (MQ=255)
                                                           aGCTATCGATTGATTGCTCAACACGTTGAAtattat                                      >  1:2288682/1‑36 (MQ=255)
                                                           aGCTATCGATTGATTGCTCAACACGTTGAAtattat                                      >  1:2704530/1‑36 (MQ=255)
                                                           aGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTACGCAGCCgg    >  1:673387/1‑70 (MQ=255)
                                                           aGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTACGCAGCCg     >  1:1271453/1‑69 (MQ=255)
                                                            gCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTACGCAGCCGGTa  >  1:2422214/1‑71 (MQ=255)
                                                            gCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTACGCAGCCGGTa  >  1:4186265/1‑71 (MQ=255)
                                                             cTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGttt              <  1:1950882/58‑1 (MQ=255)
                                                             cTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGttt              <  1:1776336/58‑1 (MQ=255)
                                                             cTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGttt              <  1:2806006/58‑1 (MQ=255)
                                                             cTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGttt              <  1:1587291/58‑1 (MQ=255)
                                                              |                                                                    
ATCCCACCTATAAAAGCGAGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTACGCAGCCGGTA  >  minE/2168044‑2168174

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: