Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,168,110 G→A 5.3% R61Q (CGA→CAA)  yrbK → conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,168,1100GA 5.3% 317.4 / 5.3 96R61Q (CGA→CAA) yrbKconserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (48/42);  new base A (3/2);  total (51/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.10e-01

Only 100 of 130 total aligned reads displayed.

ATCCCACCTATAAAAGCGAGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTACGCAGCCGGTACTTAC  >  minE/2168044‑2168179
                                                                  |                                                                     
aTCCCACCTATAAAAGCGAGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCgatt                                                                    <  1:3700301/70‑1 (MQ=255)
aTCCCACCTATAAAAGCGAGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCgatt                                                                    <  1:2410259/70‑1 (MQ=255)
  cccACCTATAAAAGCGAGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCgattgat                                                                 >  1:2570456/1‑71 (MQ=255)
  cccACCTATAAAAGCGAGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCgattgat                                                                 >  1:3642426/1‑71 (MQ=255)
  cccACCTATAAAAGCGAGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCgattgat                                                                 >  1:2093670/1‑71 (MQ=255)
         ataAAAGCGAGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCaa                                                          >  1:2797023/1‑71 (MQ=255)
          taAAAGCGAGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCaa                                                          >  1:2805253/1‑70 (MQ=255)
          taAAAGCGAGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCaa                                                          <  1:3747889/70‑1 (MQ=255)
          taAAAGCGAGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCa                                                           <  1:2462704/69‑1 (MQ=255)
            aaaGCGAGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAaca                                                        <  1:1684545/70‑1 (MQ=255)
                  aGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGaa                                                 <  1:2324060/71‑1 (MQ=255)
                  aGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGaa                                                 <  1:3826743/71‑1 (MQ=255)
                  aGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGaa                                                 <  1:4138536/71‑1 (MQ=255)
                  aGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGaa                                                 <  1:446865/71‑1 (MQ=255)
                    cATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCgattga                                                                  >  1:2283406/1‑52 (MQ=255)
                    cATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCgattga                                                                  >  1:744608/1‑52 (MQ=255)
                       aCGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAAtatta                                            >  1:4007687/1‑71 (MQ=255)
                        cGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAAtattat                                           <  1:2619735/71‑1 (MQ=255)
                        cGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAAtattat                                           >  1:3499058/1‑71 (MQ=255)
                        cGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAAtattat                                           >  1:655616/1‑71 (MQ=255)
                        cGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAAtattat                                           >  1:2814694/1‑71 (MQ=255)
                             acGCTCGTCTATAACCCAGAAGGGGCACTAAGCAATCAATCGATAGCtta                                                           >  1:3082134/1‑48 (MQ=21)
                             acGCTCGTCTATAACCCAGAAGGGGCACTAAGCAATCAATCGATAGCtta                                                           >  1:1692159/1‑48 (MQ=21)
                               gCTCTTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAat                                                >  1:635203/1‑59 (MQ=255)
                               gCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCgattg                                                                   <  1:3669022/40‑1 (MQ=255)
                               gCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCgattg                                                                   <  1:1474242/40‑1 (MQ=255)
                               gCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCgattg                                                                   <  1:456039/40‑1 (MQ=255)
                               gCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCgattg                                                                   <  1:2472736/40‑1 (MQ=255)
                               gCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGAt                                     >  1:2541305/1‑70 (MQ=255)
                                cTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAata                                               <  1:2222012/59‑1 (MQ=255)
                                cTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCa                                   <  1:300955/71‑1 (MQ=255)
                                    tCTATAACCCAGATGGGGCAGTAAGCTATCTATTGATTGCTCAACACGTTGaa                                                 <  1:1314353/53‑1 (MQ=255)
                                      tataACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAAtatta                                            >  1:3154849/1‑56 (MQ=255)
                                      tataACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAAtatta                                            >  1:3160071/1‑56 (MQ=255)
                                      tataACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAAtatta                                            >  1:3547120/1‑56 (MQ=255)
                                      tataACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAAtatta                                            >  1:890936/1‑56 (MQ=255)
                                      tataACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAAtatta                                            >  1:3141869/1‑56 (MQ=255)
                                       ataACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGtt                            >  1:4187689/1‑71 (MQ=255)
                                       ataACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGtt                            >  1:2752850/1‑71 (MQ=255)
                                         aaCCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATCCCGATCAGGCCGTTTc                          >  1:1346671/1‑71 (MQ=255)
                                          aCCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCg                         <  1:2908913/71‑1 (MQ=255)
                                               gAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGtt                    <  1:3100886/71‑1 (MQ=255)
                                                aaGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAg                                  <  1:3205267/56‑1 (MQ=255)
                                                aaGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAg                                  <  1:675807/56‑1 (MQ=255)
                                                aaGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAg                                  <  1:1643106/56‑1 (MQ=255)
                                                 aGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAAtatta                                            >  1:2901300/1‑45 (MQ=255)
                                                 aGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAAtatta                                            >  1:295164/1‑45 (MQ=255)
                                                 aGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAAtatta                                            >  1:1427102/1‑45 (MQ=255)
                                                 aGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAAtatta                                            >  1:1006318/1‑45 (MQ=255)
                                                 aGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTa                  <  1:1780166/71‑1 (MQ=255)
                                                  ggggCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGc                                >  1:394922/1‑56 (MQ=255)
                                                      cACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTc                          <  1:2121294/58‑1 (MQ=255)
                                                        cTAAGCTATCGATTGATTGCTCAACACGTTGAAtatt                                             >  1:3966166/1‑37 (MQ=255)
                                                        cTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTACGCa              <  1:3172701/68‑1 (MQ=255)
                                                        cTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTACGCAGcc           <  1:3742572/71‑1 (MQ=255)
                                                         tAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCg                                       <  1:1933912/42‑1 (MQ=255)
                                                         tAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCg                                       <  1:3141003/42‑1 (MQ=255)
                                                         tAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCg                                       <  1:1828210/42‑1 (MQ=255)
                                                         tAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTAc                 <  1:2660513/64‑1 (MQ=255)
                                                         tAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTAc                 <  1:301122/64‑1 (MQ=255)
                                                         tAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTAc                 <  1:1070935/64‑1 (MQ=255)
                                                         tAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTAc                 <  1:2737450/64‑1 (MQ=255)
                                                         tAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTACGCAGc            <  1:2417732/69‑1 (MQ=255)
                                                          aaGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGAt                                     >  1:974385/1‑43 (MQ=255)
                                                          aaGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGAt                                     >  1:1291154/1‑43 (MQ=255)
                                                          aaGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGAt                                     >  1:17617/1‑43 (MQ=255)
                                                          aaGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTACGCAGCCgg         >  1:2462023/1‑71 (MQ=255)
                                                          aaGCTATCGATTGATTGCTCAACACGGTGAATATTATTCCGAt                                     >  1:3949919/1‑43 (MQ=255)
                                                           aGCTATCGATTGATTGCTCAACACGTTGAAtattat                                           >  1:2288682/1‑36 (MQ=255)
                                                           aGCTATCGATTGATTGCTCAACACGTTGAAtattat                                           >  1:2704530/1‑36 (MQ=255)
                                                           aGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTACGCAGCCgg         >  1:673387/1‑70 (MQ=255)
                                                            gCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTACGCAGCCGGTa       >  1:4186265/1‑71 (MQ=255)
                                                             cTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGttt                   <  1:1776336/58‑1 (MQ=255)
                                                             cTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGttt                   <  1:1587291/58‑1 (MQ=255)
                                                               aTCGATTGATTGCTCAACACGTTGAATATTATTcc                                        <  1:394121/35‑1 (MQ=255)
                                                                tCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCg                         <  1:2891563/49‑1 (MQ=255)
                                                                tCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCg                         <  1:3316036/49‑1 (MQ=255)
                                                                tCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTa                  >  1:1118355/1‑56 (MQ=255)
                                                                tCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTATGCAGCCGGTACt     <  1:2737058/69‑1 (MQ=255)
                                                                tCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTACGCAGCCGGTACtt    <  1:3416076/70‑1 (MQ=255)
                                                                tCGATTATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTACGCAGCCGGt        <  1:2756013/65‑1 (MQ=255)
                                                                 cGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTa                  >  1:1037721/1‑55 (MQ=255)
                                                                 cGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTa                  >  1:2855176/1‑55 (MQ=255)
                                                                 cGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTa                  >  1:3723140/1‑55 (MQ=255)
                                                                  gattgattGCTCAACACGTTGAATATTATTCCGATCa                                   >  1:2782975/1‑37 (MQ=255)
                                                                  gattgattGCTCAACACGTTGAATATTATTCCGATCa                                   >  1:2006625/1‑37 (MQ=255)
                                                                  gattgattGCTCAACACGTTGAATATTATTCCGATCa                                   >  1:2378914/1‑37 (MQ=255)
                                                                  gattgattGCTCAACACGTTGAATATTATTCCGATCAGGc                                >  1:2872066/1‑40 (MQ=255)
                                                                  gattgattGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGt                        >  1:3241793/1‑48 (MQ=255)
                                                                  gattgattGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGt                        >  1:666930/1‑48 (MQ=255)
                                                                  gattgattGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTg                       >  1:3101474/1‑49 (MQ=255)
                                                                  gattgattGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTg                       >  1:1878678/1‑49 (MQ=255)
                                                                  gattgattGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTg                       >  1:2583402/1‑49 (MQ=255)
                                                                  gattgattGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTAc                 >  1:2574084/1‑55 (MQ=255)
                                                                  gattgattGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTAc                 >  1:4190408/1‑55 (MQ=255)
                                                                  gattgattGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTAc                 >  1:3021474/1‑55 (MQ=255)
                                                                  gattgattGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTACGCAGCCGGTACTTAc  >  1:388965/1‑70 (MQ=255)
                                                                  gattgattGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTACGCAGCCGGTACTTAc  <  1:407520/70‑1 (MQ=255)
                                                                  gattgattGCTCAACACGTTGAAAATTATTCCGATCAGGCCGTTTCGTGGTTTAc                 >  1:1483548/1‑55 (MQ=255)
                                                                  gattgattGCTCAAAACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTACGCAGCCGGTACTTAc  >  1:3857064/1‑70 (MQ=255)
                                                                  |                                                                     
ATCCCACCTATAAAAGCGAGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTACGCAGCCGGTACTTAC  >  minE/2168044‑2168179

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: