Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,168,113 T→C 5.2% L62S (TTG→TCG)  yrbK → conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,168,1130TC 5.2% 274.5 / 6.0 96L62S (TTG→TCG) yrbKconserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (48/43);  new base C (3/2);  total (51/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.71e-01

Only 100 of 136 total aligned reads displayed.

ATCCCACCTATAAAAGCGAGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTACGCAGCCGGTACTTACCAC  >  minE/2168044‑2168182
                                                                     |                                                                     
aTCCCACCTATAAAAGCGAGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCgatt                                                                       <  1:3700301/70‑1 (MQ=255)
aTCCCACCTATAAAAGCGAGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCgatt                                                                       <  1:3984918/70‑1 (MQ=255)
aTCCCACCTATAAAAGCGAGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCgatt                                                                       <  1:2777089/70‑1 (MQ=255)
  cccACCTATAAAAGCGAGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCgattgat                                                                    >  1:2093670/1‑71 (MQ=255)
          taAAAGCGAGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCaa                                                             >  1:2805253/1‑70 (MQ=255)
          taAAAGCGAGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCaa                                                             <  1:3747889/70‑1 (MQ=255)
            aaaGCGAGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAaca                                                           <  1:1684545/70‑1 (MQ=255)
                  aGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGaa                                                    <  1:446865/71‑1 (MQ=255)
                  aGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGaa                                                    <  1:3826743/71‑1 (MQ=255)
                    cATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCgattga                                                                     >  1:2283406/1‑52 (MQ=255)
                    cATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCgattga                                                                     >  1:1349472/1‑52 (MQ=255)
                       aCGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAAtatta                                               >  1:4007687/1‑71 (MQ=255)
                        cGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAAtattat                                              >  1:3499058/1‑71 (MQ=255)
                        cGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAAtattat                                              >  1:655616/1‑71 (MQ=255)
                        cGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAAtattat                                              <  1:2619735/71‑1 (MQ=255)
                        cGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAAtatta                                               >  1:688776/1‑70 (MQ=255)
                           acacGCTCGTCTATAACCCAGAAGGGGCACTAAGCAATCAATCGATAGCt                                                                <  1:3166054/50‑1 (MQ=25)
                           acacGCTCGTCTATAACCCAGAAGGGGCACTAAGCAATCAATCGATAGCt                                                                <  1:232524/50‑1 (MQ=25)
                             acGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAata                                                  >  1:3499774/1‑62 (MQ=255)
                             acGCTCGTCTATAACCCAGAAGGGGCACTAAGCAATCAATCGATAGCtta                                                              >  1:1692159/1‑48 (MQ=21)
                             acGCTCGTCTATAACCCAGAAGGGGCACTAAGCAATCAATCGATAGCtta                                                              >  1:17325/1‑48 (MQ=21)
                             acGCTCGTCTATAACCCAGAAGGGGCACTAAGCAATCAATCGATAGCtta                                                              >  1:3082134/1‑48 (MQ=21)
                               gCTCTTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAat                                                   >  1:635203/1‑59 (MQ=255)
                               gCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCgattg                                                                      <  1:3669022/40‑1 (MQ=255)
                               gCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCgattg                                                                      <  1:2472736/40‑1 (MQ=255)
                               gCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGAt                                        >  1:2541305/1‑70 (MQ=255)
                                cTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCa                                      <  1:300955/71‑1 (MQ=255)
                                    tCTATAACCCAGATGGGGCAGTAAGCTATCTATTGATTGCTCAACACGTTGaa                                                    <  1:1314353/53‑1 (MQ=255)
                                      tataACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAAtatta                                               >  1:3547120/1‑56 (MQ=255)
                                      tataACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAAtatta                                               >  1:890936/1‑56 (MQ=255)
                                      tataACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAAtatta                                               >  1:3141869/1‑56 (MQ=255)
                                      tataACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAAtatta                                               >  1:3160071/1‑56 (MQ=255)
                                          aCCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCg                            <  1:2908913/71‑1 (MQ=255)
                                           cccAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTCAATATTATTCCGATCAGGCCGTTTCg                            <  1:427989/70‑1 (MQ=255)
                                               gAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGtt                       <  1:3100886/71‑1 (MQ=255)
                                                aaGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAg                                     <  1:675807/56‑1 (MQ=255)
                                                 aGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAAtatta                                               >  1:2901300/1‑45 (MQ=255)
                                                 aGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAAtatta                                               >  1:295164/1‑45 (MQ=255)
                                                 aGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAAtatta                                               >  1:1006318/1‑45 (MQ=255)
                                                 aGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAAtatta                                               >  1:1427102/1‑45 (MQ=255)
                                                 aGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTa                     <  1:1780166/71‑1 (MQ=255)
                                                  ggggCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGc                                   >  1:394922/1‑56 (MQ=255)
                                                      cACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTc                             <  1:2121294/58‑1 (MQ=255)
                                                        cTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTACGCa                 <  1:3172701/68‑1 (MQ=255)
                                                        cTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTACGCAGcc              <  1:3742572/71‑1 (MQ=255)
                                                         tAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCg                                          <  1:3141003/42‑1 (MQ=255)
                                                         tAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCg                                          <  1:1933912/42‑1 (MQ=255)
                                                         tAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTAc                    <  1:2737450/64‑1 (MQ=255)
                                                         tAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTAc                    <  1:301122/64‑1 (MQ=255)
                                                         tAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTAc                    <  1:1070935/64‑1 (MQ=255)
                                                         tAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTAc                    <  1:2713415/64‑1 (MQ=255)
                                                         tAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTAc                    <  1:2660513/64‑1 (MQ=255)
                                                         tAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTACGCAGc               <  1:2417732/69‑1 (MQ=255)
                                                          aaGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGAt                                        >  1:3277332/1‑43 (MQ=255)
                                                          aaGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGAt                                        >  1:4169916/1‑43 (MQ=255)
                                                          aaGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGAt                                        >  1:974385/1‑43 (MQ=255)
                                                          aaGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGAt                                        >  1:1291154/1‑43 (MQ=255)
                                                          aaGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGAt                                        >  1:17617/1‑43 (MQ=255)
                                                          aaGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTACGCAGCCgg            >  1:2274826/1‑71 (MQ=255)
                                                          aaGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTACGCAGCCgg            >  1:2462023/1‑71 (MQ=255)
                                                          aaGCTATCGATTGATTGCTCAACACGGTGAATATTATTCCGAt                                        >  1:3949919/1‑43 (MQ=255)
                                                           aGCTATCGATTGATTGCTCAACACGTTGAAtattat                                              >  1:2288682/1‑36 (MQ=255)
                                                           aGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTACGCAGCCgg            >  1:673387/1‑70 (MQ=255)
                                                           aGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTACGCAGCCg             >  1:1271453/1‑69 (MQ=255)
                                                            gCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTACGCAGCCGGTa          >  1:4186265/1‑71 (MQ=255)
                                                            gCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTACGCAGCCGGTa          >  1:2422214/1‑71 (MQ=255)
                                                             cTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGttt                      <  1:2806006/58‑1 (MQ=255)
                                                             cTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGttt                      <  1:1950882/58‑1 (MQ=255)
                                                             cTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGttt                      <  1:1587291/58‑1 (MQ=255)
                                                               aTCGATTGATTGCTCAACACGTTGAATATTATTcc                                           <  1:394121/35‑1 (MQ=255)
                                                                tCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCg                            <  1:3316036/49‑1 (MQ=255)
                                                                tCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTa                     >  1:1803923/1‑56 (MQ=255)
                                                                tCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTa                     >  1:2880711/1‑56 (MQ=255)
                                                                tCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTATGCAGCCGGTACt        <  1:2737058/69‑1 (MQ=255)
                                                                tCGATTATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTACGCAGCCGGt           <  1:2756013/65‑1 (MQ=255)
                                                                 cGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTa                     >  1:660283/1‑55 (MQ=255)
                                                                 cGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTa                     >  1:2855176/1‑55 (MQ=255)
                                                                 cGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTa                     >  1:3723140/1‑55 (MQ=255)
                                                                 cGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTa                     >  1:1037721/1‑55 (MQ=255)
                                                                  gattgattGCTCAACACGTTGAATATTATTCCGATCa                                      >  1:2378914/1‑37 (MQ=255)
                                                                  gattgattGCTCAACACGTTGAATATTATTCCGATCa                                      >  1:2782975/1‑37 (MQ=255)
                                                                  gattgattGCTCAACACGTTGAATATTATTCCGATCAGGc                                   >  1:2872066/1‑40 (MQ=255)
                                                                  gattgattGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGt                           >  1:3241793/1‑48 (MQ=255)
                                                                  gattgattGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGt                           >  1:2560147/1‑48 (MQ=255)
                                                                  gattgattGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTg                          >  1:2583402/1‑49 (MQ=255)
                                                                  gattgattGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTg                          >  1:3101474/1‑49 (MQ=255)
                                                                  gattgattGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTg                          >  1:1878678/1‑49 (MQ=255)
                                                                  gattgattGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTAc                    >  1:3021474/1‑55 (MQ=255)
                                                                  gattgattGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTAc                    >  1:2574084/1‑55 (MQ=255)
                                                                  gattgattGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTAc                    >  1:4190408/1‑55 (MQ=255)
                                                                  gattgattGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTAc                    >  1:1499882/1‑55 (MQ=255)
                                                                  gattgattGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTACGCAGCCGGTACTTAc     <  1:407520/70‑1 (MQ=255)
                                                                  gattgattGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTACGCAGCCGGTACTTAc     >  1:388965/1‑70 (MQ=255)
                                                                  gattgattGCTCAACACGTTGAAAATTATTCCGATCAGGCCGTTTCGTGGTTTAc                    >  1:1483548/1‑55 (MQ=255)
                                                                  gattgattGCTCAAAACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTACGCAGCCGGTACTTAc     >  1:3857064/1‑70 (MQ=255)
                                                                   attgattgCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTACGCAGCCGGTAc         <  1:2397574/65‑1 (MQ=255)
                                                                   attgattgCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTACGCAGCCGGTAc         <  1:1784374/65‑1 (MQ=255)
                                                                    ttgattgCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTACGCAGCCGGTACTTACCAc  >  1:2673232/1‑71 (MQ=255)
                                                                    ttgattgCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTACGCAGCCGGTACTTACCAc  >  1:1759905/1‑71 (MQ=255)
                                                                     tgattgCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTACGCAGCCGGTACtt       >  1:13469/1‑65 (MQ=255)
                                                                     |                                                                     
ATCCCACCTATAAAAGCGAGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTACGCAGCCGGTACTTACCAC  >  minE/2168044‑2168182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: