Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,168,117 T→A 5.2% I63I (ATT→ATA yrbK → conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,168,1170TA 5.2% 276.1 / 6.1 96I63I (ATT→ATAyrbKconserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (48/43);  new base A (3/2);  total (51/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.11e-01

Only 100 of 123 total aligned reads displayed.

ACCTATAAAAGCGAGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTACGCAGCCGGTACTTACCAC  >  minE/2168049‑2168182
                                                                    |                                                                 
    ataAAAGCGAGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCaa                                                             >  1:2797023/1‑71 (MQ=255)
     taAAAGCGAGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCaa                                                             <  1:3747889/70‑1 (MQ=255)
     taAAAGCGAGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCaa                                                             <  1:295165/70‑1 (MQ=255)
     taAAAGCGAGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCa                                                              <  1:2462704/69‑1 (MQ=255)
       aaaGCGAGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAaca                                                           <  1:1684545/70‑1 (MQ=255)
             aGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGaa                                                    <  1:2324060/71‑1 (MQ=255)
                  aCGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAAtatta                                               >  1:4007687/1‑71 (MQ=255)
                   cGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAAtattat                                              >  1:3499058/1‑71 (MQ=255)
                   cGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAAtattat                                              >  1:2814694/1‑71 (MQ=255)
                   cGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAAtattat                                              <  1:2619735/71‑1 (MQ=255)
                   cGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAAtattat                                              >  1:655616/1‑71 (MQ=255)
                   cGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAAtatta                                               >  1:688776/1‑70 (MQ=255)
                      acacGCTCGTCTATAACCCAGAAGGGGCACTAAGCAATCAATCGATAGCt                                                                <  1:3166054/50‑1 (MQ=25)
                      acacGCTCGTCTATAACCCAGAAGGGGCACTAAGCAATCAATCGATAGCt                                                                <  1:232524/50‑1 (MQ=25)
                        acGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAata                                                  >  1:3499774/1‑62 (MQ=255)
                        acGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGa                                         <  1:3712488/71‑1 (MQ=255)
                        acGCTCGTCTATAACCCAGAAGGGGCACTAAGCAATCAATCGATAGCtta                                                              >  1:3082134/1‑48 (MQ=21)
                        acGCTCGTCTATAACCCAGAAGGGGCACTAAGCAATCAATCGATAGCtta                                                              >  1:17325/1‑48 (MQ=21)
                          gCTCTTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAat                                                   >  1:635203/1‑59 (MQ=255)
                           cTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAata                                                  <  1:2222012/59‑1 (MQ=255)
                               tCTATAACCCAGATGGGGCAGTAAGCTATCTATTGATTGCTCAACACGTTGaa                                                    <  1:1314353/53‑1 (MQ=255)
                                 tataACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAAtatta                                               >  1:3126148/1‑56 (MQ=255)
                                 tataACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAAtatta                                               >  1:890936/1‑56 (MQ=255)
                                 tataACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAAtatta                                               >  1:3154849/1‑56 (MQ=255)
                                 tataACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAAtatta                                               >  1:3141869/1‑56 (MQ=255)
                                  ataACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGtt                               >  1:2752850/1‑71 (MQ=255)
                                  ataACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGtt                               >  1:4187689/1‑71 (MQ=255)
                                    aaCCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATCCCGATCAGGCCGTTTc                             >  1:1346671/1‑71 (MQ=255)
                                     aCCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCg                            <  1:2908913/71‑1 (MQ=255)
                                      cccAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTCAATATTATTCCGATCAGGCCGTTTCg                            <  1:427989/70‑1 (MQ=255)
                                           aaGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAg                                     <  1:675807/56‑1 (MQ=255)
                                           aaGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAg                                     <  1:3205267/56‑1 (MQ=255)
                                           aaGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAg                                     <  1:1643106/56‑1 (MQ=255)
                                            aGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAAtatta                                               >  1:2901300/1‑45 (MQ=255)
                                            aGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAAtatta                                               >  1:295164/1‑45 (MQ=255)
                                            aGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAAtatta                                               >  1:1427102/1‑45 (MQ=255)
                                            aGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAAtatta                                               >  1:1006318/1‑45 (MQ=255)
                                            aGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTa                     <  1:1780166/71‑1 (MQ=255)
                                             ggggCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGc                                   >  1:3927442/1‑56 (MQ=255)
                                                 cACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTc                             <  1:2121294/58‑1 (MQ=255)
                                                   cTAAGCTATCGATTGATTGCTCAACACGTTGAAtatt                                                >  1:3966166/1‑37 (MQ=255)
                                                   cTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTACGCa                 <  1:3172701/68‑1 (MQ=255)
                                                   cTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTACGCAGcc              <  1:26190/71‑1 (MQ=255)
                                                   cTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTACGCAGcc              <  1:3742572/71‑1 (MQ=255)
                                                    tAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCg                                          <  1:3141003/42‑1 (MQ=255)
                                                    tAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCg                                          <  1:1933912/42‑1 (MQ=255)
                                                    tAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCg                                          <  1:1828210/42‑1 (MQ=255)
                                                    tAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTAc                    <  1:301122/64‑1 (MQ=255)
                                                    tAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTAc                    <  1:2713415/64‑1 (MQ=255)
                                                    tAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTAc                    <  1:1070935/64‑1 (MQ=255)
                                                    tAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTACGCAGc               <  1:2417732/69‑1 (MQ=255)
                                                     aaGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGAt                                        >  1:17617/1‑43 (MQ=255)
                                                     aaGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGAt                                        >  1:4169916/1‑43 (MQ=255)
                                                     aaGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGAt                                        >  1:974385/1‑43 (MQ=255)
                                                     aaGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTACGCAGCCgg            >  1:2462023/1‑71 (MQ=255)
                                                     aaGCTATCGATTGATTGCTCAACACGGTGAATATTATTCCGAt                                        >  1:3949919/1‑43 (MQ=255)
                                                      aGCTATCGATTGATTGCTCAACACGTTGAAtattat                                              >  1:2288682/1‑36 (MQ=255)
                                                      aGCTATCGATTGATTGCTCAACACGTTGAAtattat                                              >  1:2704530/1‑36 (MQ=255)
                                                      aGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTACGCAGCCg             >  1:1271453/1‑69 (MQ=255)
                                                       gCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTACGCAGCCGGTa          >  1:4186265/1‑71 (MQ=255)
                                                       gCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTACGCAGCCGGTa          >  1:2422214/1‑71 (MQ=255)
                                                        cTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGttt                      <  1:1950882/58‑1 (MQ=255)
                                                        cTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGttt                      <  1:2806006/58‑1 (MQ=255)
                                                        cTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGttt                      <  1:1587291/58‑1 (MQ=255)
                                                          aTCGATTGATTGCTCAACACGTTGAATATTATTcc                                           <  1:394121/35‑1 (MQ=255)
                                                           tCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCg                            <  1:3316036/49‑1 (MQ=255)
                                                           tCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTa                     >  1:1118355/1‑56 (MQ=255)
                                                           tCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTa                     >  1:2880711/1‑56 (MQ=255)
                                                           tCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTa                     >  1:1803923/1‑56 (MQ=255)
                                                           tCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTATGCAGCCGGTACt        <  1:2737058/69‑1 (MQ=255)
                                                           tCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTACGCAGCCGGTACtt       <  1:3416076/70‑1 (MQ=255)
                                                           tCGATTATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTACGCAGCCGGt           <  1:2756013/65‑1 (MQ=255)
                                                            cGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTa                     >  1:3723140/1‑55 (MQ=255)
                                                            cGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTa                     >  1:660283/1‑55 (MQ=255)
                                                            cGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTa                     >  1:2855176/1‑55 (MQ=255)
                                                            cGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTa                     >  1:1037721/1‑55 (MQ=255)
                                                             gattgattGCTCAACACGTTGAATATTATTCCGATCa                                      >  1:3059440/1‑37 (MQ=255)
                                                             gattgattGCTCAACACGTTGAATATTATTCCGATCa                                      >  1:3802804/1‑37 (MQ=255)
                                                             gattgattGCTCAACACGTTGAATATTATTCCGATCa                                      >  1:2006625/1‑37 (MQ=255)
                                                             gattgattGCTCAACACGTTGAATATTATTCCGATCa                                      >  1:2782975/1‑37 (MQ=255)
                                                             gattgattGCTCAACACGTTGAATATTATTCCGATCa                                      >  1:2378914/1‑37 (MQ=255)
                                                             gattgattGCTCAACACGTTGAATATTATTCCGATCAGGc                                   >  1:2872066/1‑40 (MQ=255)
                                                             gattgattGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGt                           >  1:3241793/1‑48 (MQ=255)
                                                             gattgattGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGt                           >  1:666930/1‑48 (MQ=255)
                                                             gattgattGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGt                           >  1:2560147/1‑48 (MQ=255)
                                                             gattgattGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTg                          >  1:3101474/1‑49 (MQ=255)
                                                             gattgattGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTg                          >  1:2583402/1‑49 (MQ=255)
                                                             gattgattGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTAc                    >  1:2574084/1‑55 (MQ=255)
                                                             gattgattGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTAc                    >  1:4190408/1‑55 (MQ=255)
                                                             gattgattGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTAc                    >  1:3021474/1‑55 (MQ=255)
                                                             gattgattGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTAc                    >  1:1499882/1‑55 (MQ=255)
                                                             gattgattGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTACGCAGCCGGTACTTAc     <  1:407520/70‑1 (MQ=255)
                                                             gattgattGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTACGCAGCCGGTACTTAc     >  1:388965/1‑70 (MQ=255)
                                                             gattgattGCTCAAAACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTACGCAGCCGGTACTTAc     >  1:3857064/1‑70 (MQ=255)
                                                              attgattgCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTACGCAGCCGGTAc         <  1:1784374/65‑1 (MQ=255)
                                                               ttgattgCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTACGCAGCCGGTACTTACCAc  >  1:2673232/1‑71 (MQ=255)
                                                               ttgattgCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTACGCAGCCGGTACTTACCAc  >  1:1759905/1‑71 (MQ=255)
                                                                tgattgCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTACGCAGCCGGTACtt       >  1:171814/1‑65 (MQ=255)
                                                                tgattgCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTACGCAGCCGGTACtt       >  1:13469/1‑65 (MQ=255)
                                                                 gattgCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTc                             >  1:1689970/1‑42 (MQ=255)
                                                                    |                                                                 
ACCTATAAAAGCGAGCATACGGACACGCTCGTCTATAACCCAGAAGGGGCACTAAGCTATCGATTGATTGCTCAACACGTTGAATATTATTCCGATCAGGCCGTTTCGTGGTTTACGCAGCCGGTACTTACCAC  >  minE/2168049‑2168182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: