| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,262,618 | C→T | 44.3% | intergenic (‑227/+186) | rplJ ← / ← rplA | 50S ribosomal subunit protein L10/50S ribosomal subunit protein L1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,262,618 | 0 | C | T | 44.3% | 54.6 / 163.7 | 122 | intergenic (‑227/+186) | rplJ/rplA | 50S ribosomal subunit protein L10/50S ribosomal subunit protein L1 |
| Reads supporting (aligned to +/- strand): ref base C (31/37); new base T (27/27); total (58/64) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.16e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.74e-01 | |||||||||||
Only 100 of 122 total aligned reads displayed.
AAACTCCTGCGGTCTTCGACGGAGGCCTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATCCGTCGCTATTCAGCTTGTGACGGCGTTGGCTTCCTTCATTCGCACCAGTCACATAGTTAGCTATGCT > minE/2262554‑2262685 | aaaCTCCTGCGGTCTTCGACGGAGGCCTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATCCGTCGc > 1:3139698/1‑71 (MQ=255)aaaCTCCTGCGGTCTTCGACGGAGGCCTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATCCGTCGc > 1:2008452/1‑71 (MQ=255) aCTCCTGCGGTCTTCGACGGAGGCCTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATTCGTCGCTa > 1:3472769/1‑71 (MQ=255) aCTCCTGCGGTCTTCGACGGAGGCCTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATTCGTCGCTa > 1:3467864/1‑71 (MQ=255) aCTCCTGCGGTCTTCGACGGAGGCCTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATTCGTCGCTa > 1:3949991/1‑71 (MQ=255) aCTCCTGCGGTCTTCGACGGAGGCCTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATTCGTCGCTa > 1:3442572/1‑71 (MQ=255) aCTCCTGCGGTCTTCGACGGAGGCCTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATTCGTCGCTa > 1:4077552/1‑71 (MQ=255) aCTCCTGCGGTCTTCGACGGAGGCCTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATCCGTCGCTa > 1:2563192/1‑71 (MQ=255) cTGCGGTCTTCGACGGAGGCCTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATTCGTCGCTATTc < 1:638948/70‑1 (MQ=255) cTGCGGTCTTCGACGGAGGCCTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATTCGTCGCTATTc < 1:4133027/70‑1 (MQ=255) cTGCGGTCTTCGACGGAGGCCTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATCCGTCGCTATTc < 1:1899460/70‑1 (MQ=255) cTGCGGTCTTCGACGGAGGCCTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATCCGTCGCTATTc < 1:1549216/70‑1 (MQ=255) cTGCGGTCTTCGACGGAGGCCTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATCCGTCGCTATTc < 1:3204532/70‑1 (MQ=255) cGGTCTTCGACGGAGGCCTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATTCGTCGCTATTCAGCt > 1:3337569/1‑71 (MQ=255) cGGTCTTCGACGGAGGCCTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATCCGTCGCTATTCAGCt < 1:3476346/71‑1 (MQ=255) cGGTCTTCGACGGAGGCCTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATCCGTCGCTATTCAGCt < 1:3589828/71‑1 (MQ=255) cGGTCTTCGACGGAGGCCTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATCCGTCGCTATTCAGCt < 1:3877335/71‑1 (MQ=255) cGGTCTTCGACGGAGGCCTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATCCGTCGCTATTCAGCt < 1:3709879/71‑1 (MQ=255) tCTTCGACGGAGGCCTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATTCGTCGCTATTCAGCTtgt < 1:4216531/71‑1 (MQ=255) tCTTCGACGGAGGCCTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATCCGTCGCTATTCAGCTtgt < 1:845034/71‑1 (MQ=255) cTTCGACGGAGGCCTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATTCGTCGCTATTCa > 1:755305/1‑64 (MQ=255) cTTCGACGGAGGCCTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATTCGTCGCTATTCa > 1:352981/1‑64 (MQ=255) ttCGACGGAGGCCTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATCCGTCGCTATTCAGCTTGTGa > 1:1150404/1‑71 (MQ=255) cGACGGAGGCCTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATCCGTCGCTATTCAGCTTGTGACg > 1:3999784/1‑71 (MQ=255) aCGGAGGCCTGGATAGGCGAGGCTCCAACGAACAAATCTTTTCTATCCGTCGCTATTCAGCTTGTGACGGc < 1:3116270/71‑1 (MQ=255) aCGGAGGCCTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATTCGTCGCTATTCAGCTTGTGACGGc < 1:2114920/71‑1 (MQ=255) aCGGAGGCCTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATCCGTCGCTATTCAGCTTGTGACGGc < 1:2181603/71‑1 (MQ=255) aCGGAGGCCTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATCCGTCGCTATTCAGCTTGTGACGGc < 1:2312448/71‑1 (MQ=255) ggAGGCCTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATCCGTCGCTATTCAGCTTGTGACGGCGt > 1:1852429/1‑71 (MQ=255) ggAGGCCTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATCCGTCGCTATTCAGCTTGTGACGGCGt > 1:3140158/1‑71 (MQ=255) gAGGCCTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATTCGTCGCTATTCAGCTTGTGACGGCGtt > 1:3211794/1‑71 (MQ=255) ggCCTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATTCGTCGCTATTCAGCTTGTGACGGCGTTgg > 1:2992846/1‑71 (MQ=255) ggCCTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATTCGTCGCTATTCAGCTTGTGACGGCGTTgg > 1:1726755/1‑71 (MQ=255) ggCCTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATTCGTCGCTATTCAGCTTGTGACGGCGTTg > 1:838259/1‑70 (MQ=255) tGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATCCGTCGCTATTCAGCTtgt < 1:660952/56‑1 (MQ=255) tGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATCCGTCGCTATTCAGCTtgt < 1:1303261/56‑1 (MQ=255) tGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATCCGTCGCTATTCAGCTTGTGACgg < 1:4166707/61‑1 (MQ=255) tGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATCCGTCGCTATTCAGCTTGTGACgg < 1:3399560/61‑1 (MQ=255) gATAGGCCAGGCTCCAACGAACAAATCTTTTCTATCCGTCGCTATTCAGCTTGTGa > 1:3526686/1‑56 (MQ=255) gATAGGCCAGGCTCCAACGAACAAATCTTTTCTATCCGTCGCTATTCAGCTTGTGa > 1:1594534/1‑56 (MQ=255) gATAGGCCAGGCTCCAACGAACAAATCTTTTCTATCCGTCGCTATTCAGCTTGTGa > 1:1796932/1‑56 (MQ=255) gATAGGCCAGGCTCCAACGAACAAATCTTTTCTATCCGTCGCTATTCAGCTTGTGACGGCg > 1:2142683/1‑61 (MQ=255) gATAGGCCAGGCTCCAACGAACAAATCTTTTCTATCCGTCGCTATTCAGCTTGTGACGGCg > 1:3921209/1‑61 (MQ=255) tAGGCCAGGCTCCAACGAACAAATCTTTTCTATCCGTCGCTATTCAGCTTGTGACGGCGTTGGcttcctt > 1:2674767/1‑70 (MQ=255) ggCCAGGCTCCAACGAACAAATCTTTTCTATTCGTCGCTATTCAGCTTGTGACGGCGTTGGCTTCCTTCa < 1:907341/70‑1 (MQ=255) ggCCAGGCTCCAACGAACAAATCTTTTCTATCCGTCGCTATTCAGCTTGTGACGGCGTTGGCTTCCTTCa < 1:551433/70‑1 (MQ=255) ggCCAGGCTCCAACGAACAAATCTTTTCTATCCGTCGCTATTCAGCTTGTGACGGCGTTGGCTTCCTTCAt < 1:2710854/71‑1 (MQ=255) cAGGCTCCAACGAACAAATCTTTTCTATTCGTCGCTATTCAGCTTGTGACGGCGTTGGCTTCCTTCATTCg < 1:4072936/71‑1 (MQ=255) cAGGCTCCAACGAACAAATCTTTTCTATTCGTCGCTATTCAGCTTGTGACGGCGTTGGCTTCCTTCATTCg < 1:1090941/71‑1 (MQ=255) cAGGCTCCAACGAACAAATCTTTTCTATTCGTCGCTATTCAGCTTGTGACGGCGTTGGCTTCCTTCATTCg < 1:2145739/71‑1 (MQ=255) aGGCTCCAACGAACAAATCTTTTCTATTCGTCGCTATTCAGCTTGTGACGGCGTTGGCTTCCTTCATTCg < 1:640318/70‑1 (MQ=255) ggCTCCAACGAACAAATCTTTTCTATCCGTCGCTATTCAGCTTGTGACGGc < 1:2435917/51‑1 (MQ=255) gCTCCAACGAACAAATCTTTTCTATCCGTCGCTATTCAGCTTGTGACGGCGTTGGCTTCCTTCATTCGCAc < 1:4248308/71‑1 (MQ=255) gCTCCAACGAACAAATCTTTTCTATCCGTCGCTATTCAGCTTGTGACGGCGTTGGCTTCCTTCATTCGCAc < 1:2718153/71‑1 (MQ=255) cTCCAACGAACAAATCTTTTCTATCCGTCGCTATTCAGCTTGTGACGGCGTTGGCTTCCTTCATTCGCAc > 1:1383343/1‑70 (MQ=255) cTCCAACGAACAAATCTTTTCTATCCGTCGCTATTCAGCTTGTGACGGCGTTGGCTTCCTTCATTCGCAc > 1:3647861/1‑70 (MQ=255) tCCAACGAACAAATCTTTTCTATTCGTCGCTATTCAGCTtgt < 1:2947311/42‑1 (MQ=255) tCCAACGAACAAATCTTTTCTATTCGTCGCTATTCAGCTtgt < 1:1505943/42‑1 (MQ=255) tCCAACGAACAAATCTTTTCTATTCGTCGCTATTCAGCTtgt < 1:825366/42‑1 (MQ=255) tCCAACGAACAAATCTTTTCTATTCGTCGCTATTCAGCTtgt < 1:899730/42‑1 (MQ=255) tCCAACGAACAAATCTTTTCTATCCGTCGCTATTCAGCTTGTGACGGCGTTGGCTTCCTTCATTCGCAc > 1:722691/1‑69 (MQ=255) cAACGAACAAATCTTTTCTATTCGTCGCTATTCAGCTtgt < 1:2567856/40‑1 (MQ=255) cAACGAACAAATCTTTTCTATTCGTCGCTATTCAGCTtgt < 1:2694916/40‑1 (MQ=255) cAACGAACAAATCTTTTCTATTCGTCGCTATTCAGCTtgt < 1:3311988/40‑1 (MQ=255) cAACGAACAAATCTTTTCTATTCGTCGCTATTCAGCTtgt < 1:3600828/40‑1 (MQ=255) cAACGAACAAATCTTTTCTATTCGTCGCTATTCAGCTtgt < 1:1071251/40‑1 (MQ=255) cAACGAACAAATCTTTTCTATTCGTCGCTATTCAGCTtgt < 1:1202826/40‑1 (MQ=255) cAACGAACAAATCTTTTCTATTCGTCGCTATTCAGCTTGTGa > 1:1587774/1‑42 (MQ=255) cAACGAACAAATCTTTTCTATTCGTCGCTATTCAGCTTGTGACGGCGTTGGCTTCCTTCATTCGCACCAGt < 1:3183914/71‑1 (MQ=255) cAACGAACAAATCTTTTCTATCCGTCGCTATTCAGCTTGTGACGGCGt > 1:267687/1‑48 (MQ=255) cAACGAACAAATCTTTTCTATCCGTCGCTATTCAGCTTGTGACGGCGt > 1:1770694/1‑48 (MQ=255) aCGAACAAATCTTTTCTATTCGTCGCTATTCAGCTTGTGACGGCGTTGGCTTCCTTCATTCGCACCAGTca > 1:2467834/1‑71 (MQ=255) aCGAACAAATCTTTTCTATTCGTCGCTATTCAGCTTGTGACGGCGTTGGCTTCCTTCATTCGCACCAGTca > 1:2799332/1‑71 (MQ=255) aCGAACAAATCTTTTCTATTCGTCGCTATTCAGCTTGTGACGGCGTTGGCTTCCTTCATTCGCACCAGGca > 1:3294920/1‑71 (MQ=255) cGAACAAATCTTTTCTATCCGTCGCTATTCAGCTTGTGACGGc < 1:268291/43‑1 (MQ=255) gAACAAATCTTTTCTATCCGTCGCTATTCAGCTTGTGACGGCGTTg < 1:4207843/46‑1 (MQ=255) aaCAAATCTTTTCTATTCGTCGCTATTCAGCTtgt < 1:3953730/35‑1 (MQ=255) aaCAAATCTTTTCTATTCGTCGCTATTCAGCTTGTGACGGCGTTGGCTTCCTTCATTCGCACCAGTCACAt < 1:2563545/71‑1 (MQ=255) aCAAATCTTTTCTATTCGTCGCTATTCAGCTTGTGACGGCGTTGGCTTCCTTCATTCGCACCAGTcaca > 1:734699/1‑69 (MQ=255) aCAAATCTTTTCTATTCGTCGCTATTCAGCTTGTGACGGCGTTGGCTTCCTTCATTCGCACCAGTcaca > 1:1251288/1‑69 (MQ=255) aCAAATCTTTTCTATTCGTCGCTATTCAGCTTGTGACGGCGTTGGCTTCCTTCATTCGCACCAGTcaca > 1:1263795/1‑69 (MQ=255) aCAAATCTTTTCTATCCGTCGCTATTCAGCTTGTGACGGCGTTGGc > 1:2263769/1‑46 (MQ=255) aCAAATCTTTTCTATCCGTCGCTATTCAGCTTGTGACGGCGTTGGc > 1:3726772/1‑46 (MQ=255) cAAATCTTTTCTATCCGTCGCTATTCAGCTTGTGACGGCGTTGGCTTCCTTCATTCg < 1:2136848/57‑1 (MQ=255) cAAATCTTTTCTATCCGTCGCTATTCAGCTTGTGACGGCGTTGGCTTCCTTCATTCg < 1:3837447/57‑1 (MQ=255) aaTCTTTTCTATTCGTCGCTATTCAGCTTGTGACGGCGTTGGCTTCCTTCATTCGCACCAGTCACATa < 1:93713/68‑1 (MQ=255) aaTCTTTTCTATCCGTCGCTATTCAGCTTGTGACGGCGTTGGCTTCCTTCATTCGCACCAGTCACATAGtt < 1:2277566/71‑1 (MQ=255) aaTCTTTTCTATCCGTCGCTATTCAGCTTGTGACGGCGTTGGCTTCCTTCATTCGCACCAGTCACATAGtt < 1:2907845/71‑1 (MQ=255) cTTTTCTATTCGTCGCTATTCAGCTTGTGACGGCGTTGGCTTCCTTCATTCGCa > 1:3406001/1‑54 (MQ=255) ttttCTATTCGTCGCTATTCAGCTTGTGACGGCGTTGGcttcctt < 1:4186979/45‑1 (MQ=255) ttttCTATCCGTCGCTATTCAGCTTGTGACGGCGTTGGCTTCCTTCATTCGCACCAGTCACATAGTTAGc > 1:1217421/1‑70 (MQ=255) ttCTATTCGTCGCTATTCAGCTTGTGACGGCGTTGGCTTCCTTCATTCGCACCAGTCACATAGTTAGCTAt > 1:1362328/1‑71 (MQ=255) ttCTATTCGTCGCTATTCAGCTTGTGACGGCGTTGGCTTCCTTCATTCGCACCAGTCACATAGTTAGCTAt > 1:1931135/1‑71 (MQ=255) ttCTATTCGTCGCTATTCAGCTTGTGACGGCGTTGGCTTCCTTCATTCGCACCAGTCACATAGTTAGCTAt > 1:2550898/1‑71 (MQ=255) ttCTATTCGTCGCTATTCAGCTTGTGACGGCGTTGGCTTCCTTCATTCGCACCAGTCACATAGTTAGCTAt > 1:2884953/1‑71 (MQ=255) ttCTATCCGTCGCTATTCAGCTTGTGACGGCGTTGGCTTCCTTCATTCGCACCAGTCACATAGTTAGc < 1:4118074/68‑1 (MQ=255) cTATCCGTCGCTATTCAGCTTGTGACGGCGTTGGCTTCCTTCATTCGCACCAGTCACATAGTTAGc < 1:1443257/66‑1 (MQ=255) cTATCCGTCGCTATTCAGCTTGTGACGGCGTTGGCTTCCTTCATTCGCACCAGTCACATAGTTAGCTATGc > 1:2897516/1‑71 (MQ=255) tCCGTCGCTATTCAGCTTGTGACGGCGTTGGCTTCCTTCATTCGCAc < 1:3919194/47‑1 (MQ=255) tCCGTCGCTATTCAGCTTGTGACGGCGTTGGCTTCCTTCATTCGCACCAGTCACATAGTTAGCTATGCt < 1:1504192/69‑1 (MQ=255) | AAACTCCTGCGGTCTTCGACGGAGGCCTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATCCGTCGCTATTCAGCTTGTGACGGCGTTGGCTTCCTTCATTCGCACCAGTCACATAGTTAGCTATGCT > minE/2262554‑2262685 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |