| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,283,783 | G→T | 41.5% | L39M (CTG→ATG) | oxyR ← | DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,283,783 | 0 | G | T | 41.5% | 79.4 / 60.0 | 65 | L39M (CTG→ATG) | oxyR | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base G (16/22); new base T (16/11); total (32/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.13e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.56e-01 | |||||||||||
ATTCCCGCCTGGGTGAACAACACTTTACGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCAGCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTAACGTGGCAGGAATCTGCCGCACGCCGAAAATGGC > minE/2283713‑2283851 | aTTCCCGCCTGGGTGAACAACACTTTACGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCAg > 1:41328/1‑71 (MQ=255) ttCCCGCCTGGGTGAACAACACTTTACGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCAg > 1:219679/1‑70 (MQ=255) ttCCCGCCTGGGTGAACAACACTTTACGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCAGc > 1:2489353/1‑71 (MQ=255) ttCCCGCCTGGGTGAACAACACTTTACGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCAGc > 1:225218/1‑71 (MQ=255) cccGCCTGGGTGAACAACACTTTACGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCATCt > 1:3571837/1‑70 (MQ=255) cccGCCTGGGTGAACAACACTTTACGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCAGCt < 1:245560/70‑1 (MQ=255) cccGCCTGGGTGAACAACACTTTACGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCAGCt < 1:1875117/70‑1 (MQ=255) ccTGGGTGAACAACACTTTACGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCAGCTTACGa < 1:1908779/71‑1 (MQ=255) ccTGGGTGAACAACACTTTACGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCAGCTTACGa < 1:2225863/71‑1 (MQ=255) ggTGAACAACACTTTACGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCAGCTTACGAAtt < 1:3802265/70‑1 (MQ=255) gTGAACAACACTTTACGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCATCTTACGAATTTg < 1:4216951/71‑1 (MQ=255) gTGAACAACACTTTACGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCATCTTACGAATTTg < 1:3938871/71‑1 (MQ=255) caacaCTTTACGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCATCTTACGAATTTGCCCGc > 1:2051849/1‑71 (MQ=255) caacaCTTTACGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCAGCTTACGAATTTg < 1:504835/66‑1 (MQ=255) aacaCTTTACGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCAGCTTACGAATTTGCCCGCt < 1:3957424/71‑1 (MQ=255) acacTTTACGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCAGCTTACGAATTTGCCCGCt < 1:433997/70‑1 (MQ=255) cacTTTACGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCATCTTACGAATTTGCCCGCTaa < 1:79954/71‑1 (MQ=255) cacTTTACGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCATCTTACGAATTTGCCCGCTaa < 1:762580/71‑1 (MQ=255) cacTTTACGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCATCTTACGAATTTGCCCGCTaa < 1:6538/71‑1 (MQ=255) cTTTACGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCAGCTTACGAATTTGCCCGCTAAGc > 1:3094224/1‑71 (MQ=255) cTTTACGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCAGCTTACGAATTTGCCCGCTAAGc > 1:987935/1‑71 (MQ=255) cTTTACGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCAGCTTACGAATTTGCCCGCTAAGc > 1:1737133/1‑71 (MQ=255) tACGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCAGCTTACGAATTTGCCCGCTAAGCGTc > 1:4241247/1‑71 (MQ=255) tACGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCAGCTTACGAATTTGCCCGCTAAGCGTc > 1:3703378/1‑71 (MQ=255) tACGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCAGCTTACGAATTTGCCCGCTAAGCGTc > 1:3819799/1‑71 (MQ=255) tACGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCAGCTTACGAATTTGCCCGCTAAGCGTc > 1:2055474/1‑71 (MQ=255) tACGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCAGCTTACGAATTTGCCCGCTAAGCGTc > 1:1749532/1‑71 (MQ=255) tACGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCAGCTTACGAATTTGCCCGCTAAGCGTc > 1:4118125/1‑71 (MQ=255) cGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCAGCTTACGAATTTGCCCGCTAAGCGTCgg < 1:2677352/71‑1 (MQ=255) cGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCAGCTTACGAATTTGCCCGCTAAGCGTCgg < 1:2898149/71‑1 (MQ=255) gCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCAGCTTACGAATTTGCCCGCTAAGCGTCggct < 1:3889375/71‑1 (MQ=255) gTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCATCTTACGAATTTGCCCGCTAAGCGTCggctggct < 1:3416762/71‑1 (MQ=255) gTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCATCTTACGAATTTGCCCGCTAAGCGTCggctggc > 1:2637834/1‑70 (MQ=255) gTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCAGCTTACGAATTTGCCCGCTAAGCGTCggctggct < 1:3945337/71‑1 (MQ=255) gTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCAGCTTACGAATTTGCCCGCTAAGCGTCggctggct < 1:2446381/71‑1 (MQ=255) cGCTCCAGCAACATCACGCCCAGCTCATCTTCCATCTTACGAATTTGCCCGc > 1:3297645/1‑52 (MQ=255) cGCTCCAGCAACATCACGCCCAGCTCATCTTCCATCTTACGAATTTGCCCGc > 1:3821216/1‑52 (MQ=255) cGCTCCAGCAACATCACGCCCAGCTCATCTTCCATCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTAAc > 1:361850/1‑71 (MQ=255) gCTCCAGCAACATCACGCCCAGCTCATCTTCCAGCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTAACg < 1:1529828/71‑1 (MQ=255) cTCCATCAACATCACGCCCAGCTCATCTTCCATCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTAACGt < 1:2540098/71‑1 (MQ=255) cTCCAGCAACATCACGCCCAGCTCATCTTCCAGCTTACGAATTTg < 1:1722005/45‑1 (MQ=255) cTCCAGCAACATCACGCCCAGCTCATCTTCCAGCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTAACGt < 1:3393218/71‑1 (MQ=255) cTCCAGCAACATCACGCCCAGCTCATCTTCCAGCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTAACGt < 1:2015334/71‑1 (MQ=255) ccAGCAACATCACGCCCAGCTCATCTTCCATCTTAc > 1:2404437/1‑36 (MQ=255) ccAGCAACATCACGCCCAGCTCATCTTCCATCTTAc > 1:2948156/1‑36 (MQ=255) aaCATCACGCCCAGCTCATCTTCCATCTTACGAATTTGCCCGCTAAGCGTCggctggct < 1:255335/59‑1 (MQ=255) aTCACGCCCAGCTCATCTTCCATCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTAACGTGGCAGGAATc < 1:591425/71‑1 (MQ=255) aTCACGCCCAGCTCATCTTCCAGCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTAACGTGGCAGGAATc > 1:3533093/1‑71 (MQ=255) aTCACGCCCAGCTCATCTTCCAGCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTAACGTGGCAGGAATc > 1:3370363/1‑71 (MQ=255) aCGCCCAGCTCATCTTCCATCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTAACGTGGCAGGAATCTg > 1:4124436/1‑70 (MQ=255) aCGCCCAGCTCATCTTCCATCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTAACGTGGCAGGAATCTGc > 1:1550806/1‑71 (MQ=255) aCGCCCAGCTCATCTTCCAGCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTAACGTGGCAGGAATCTGc > 1:2930986/1‑71 (MQ=255) gCCCAGCTCATCTTCCATCTTACGAATTTGCCCGCTAAGCGTCGGCTTGCTAACGTGGCAGGAATCTGCCg > 1:2497178/1‑71 (MQ=255) gCCCAGCTCATCTTCCATCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTAACGTGGCAGGAATCTGCCg > 1:3693620/1‑71 (MQ=255) gCCCAGCTCATCTTCCATCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTAACGTGGCAGGAATCTGCCg > 1:3009912/1‑71 (MQ=255) gCCCAGCTCATCTTCCATCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTAACGTGGCAGGAATCTGCCg > 1:1924404/1‑71 (MQ=255) gCCCAGCTCATCTTCCATCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTAACGTGGCAGGAATCTGCCg > 1:1919475/1‑71 (MQ=255) gCCCAGCTCATCTTCCATCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTAACGTGGCAGGAATCTGCCg > 1:997889/1‑71 (MQ=255) ccAGCTCATCTTCCATCTTACGAATTTGCCCGCTAAGCTTCGGCTGGCTAACGTGGCAGGAATCTGCCGCa < 1:2847224/71‑1 (MQ=255) gCTCATCTTCCAGCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTAACGTGGCAGGa < 1:3857927/58‑1 (MQ=255) gCTCATCTTCCAGCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTAACGTCGCAGGa < 1:9527/58‑1 (MQ=255) cATCTTCCAGCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTAACGTGGCAGGAATCTGCCGCACGCCg > 1:428218/1‑70 (MQ=255) aTCTTCCATCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTAACGTGGCAGGAATCTGCCGCACGCCGaa < 1:277312/71‑1 (MQ=255) tCCAGCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTAAc < 1:229464/41‑1 (MQ=255) ccAGCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTAACGTGGCAGGAATCTGCCGCACGCCGAAAATgg > 1:660684/1‑71 (MQ=255) cAGCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTAACGTGGCAGGAATCTGCCGCACGCCGAAAATGGc < 1:3982552/71‑1 (MQ=255) cAGCTTACGAATTTGCCCGCTAAGCGGCGGCTGGCTAACGTGGCAGGAATCTGCCGCACGCCGAAAATGGc < 1:2526050/71‑1 (MQ=255) | ATTCCCGCCTGGGTGAACAACACTTTACGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCAGCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTAACGTGGCAGGAATCTGCCGCACGCCGAAAATGGC > minE/2283713‑2283851 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |