| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,321,698 | G→A | 52.7% | T93I (ACC→ATC) | cdh ← | CDP‑diacylglycerol phosphotidylhydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,321,698 | 0 | G | A | 52.7% | 8.0 / 166.4 | 110 | T93I (ACC→ATC) | cdh | CDP‑diacylglycerol phosphotidylhydrolase |
| Reads supporting (aligned to +/- strand): ref base G (29/23); new base A (30/28); total (59/51) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.05e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.27e-01 | |||||||||||
Only 100 of 119 total aligned reads displayed.
TTTTTTGCTCATAAAATCACGCGCCTGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAGATATTGCAGTGG > minE/2321634‑2321759 | ttttttGCTCATAAAATCACGCGCCTGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGATcaac > 1:2109146/1‑70 (MQ=255)ttttttGCTCATAAAATCACGCGCCTGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGATcaac > 1:3850185/1‑70 (MQ=255) cATAAAATCACGCGCCTGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGATCAACAAAGGACttt > 1:3672574/1‑71 (MQ=255) cATAAAATCACGCGCCTGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGATCAACAAAGGACttt > 1:1610972/1‑71 (MQ=255) cATAAAATCACGCGCCTGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGATCAACAAAGGACttt > 1:3725904/1‑71 (MQ=255) aTAAAATCACGCGCCTGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACttt < 1:1684318/70‑1 (MQ=255) aTAAAATCACGCGCCTGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTc < 1:3291532/71‑1 (MQ=255) aTAAAATCACGCGCCTGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTc < 1:2168477/71‑1 (MQ=255) aTAAAATCACGCGCCTGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTc < 1:3738189/71‑1 (MQ=255) aTAAAATCACGCGCCTGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTc < 1:3450397/71‑1 (MQ=255) aTAAAATCACGCGCCTGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGATCAACAAAGGACTTTc < 1:733938/71‑1 (MQ=255) aTAAAATCACGCGCCTGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGATCAACAAAGGACTTTc < 1:1197052/71‑1 (MQ=255) tAAAATCACGCGCCTGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACttt < 1:1095522/69‑1 (MQ=255) tAAAATCACGCGCCTGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGATca < 1:2149672/57‑1 (MQ=255) tAAAATCACGCGCCTGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGATCAACAAAGGACttt > 1:3158404/1‑69 (MQ=255) aaaaTCACGCGCCTGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGGt > 1:1406940/1‑54 (MQ=255) aaaaTCACGCGCCTGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGGt > 1:3687996/1‑54 (MQ=255) aaaaTCACGCGCCTGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGGt > 1:4001391/1‑54 (MQ=255) aaaaTCACGCGCCTGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGGt > 1:1249686/1‑54 (MQ=255) aaaTCACGCGCCTGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCa > 1:2648547/1‑69 (MQ=255) aaaTCACGCGCCTGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCa > 1:2678968/1‑69 (MQ=255) aaaTCACGCGCCTGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCa > 1:3930185/1‑69 (MQ=255) aaaTCACGCGCCTGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGATCAACAAAGGACTTTCAg > 1:910632/1‑70 (MQ=255) aaTCACGCGCCTGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGATCAACAAAgg < 1:4245841/61‑1 (MQ=255) tCACGCGCCTGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGATCAACAAAGGACt < 1:1102513/62‑1 (MQ=255) tCACGCGCCTGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGATCAACAAAGGACt < 1:2659281/62‑1 (MQ=255) tCACGCGCCTGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGATCAACAAAGGACTTTCAGTAcc < 1:3950203/71‑1 (MQ=255) tCACGCGCCTGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGATCAACAAAGGACTTTCAGTAcc < 1:800316/71‑1 (MQ=255) cACGCGCCTGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGATCAACAAAGGACTTTCAGTACCg > 1:1534483/1‑71 (MQ=255) aCGCGCCTGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGATCAACAAAGGACt < 1:2184736/60‑1 (MQ=255) gcCTGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGATCAACAAAGGACTTTCAGTACCGTTAat < 1:2332225/71‑1 (MQ=255) tGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGATCAACAAAGGACTTTCAGTACCGtt < 1:3816701/65‑1 (MQ=255) gccaagccaaCCAAAAGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAatacga > 1:3713420/1‑71 (MQ=255) gccaagccaaCCAAAAGAAGTTCGGCGTTGAAGGATCGATCAACAAAGCACTTTCAGTACCGTTAatacga > 1:2799086/1‑71 (MQ=255) ccaagccaaCCAAAAGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAatacga > 1:484503/1‑70 (MQ=255) ccaagccaaCCAAAAGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAatacga > 1:2417723/1‑70 (MQ=255) ccaagccaaCCAAAAGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAatacg > 1:1283836/1‑69 (MQ=255) ccaagccaaCCAAAAGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAatac < 1:2034026/68‑1 (MQ=255) ccaagccaaCCAAAAGAAGTTCGGCGTTGAAGGATCGATCAACAAAGGACTTTCAGTACCGTTAatacga > 1:262528/1‑70 (MQ=255) ccaagccaaCCAAAAGAAGTTCGGCGTTGAAGGATCGATCAACAAAGGACTTTCAGTACCGTTAatacga > 1:4240471/1‑70 (MQ=255) ccaagccaaCCAAAAGAAGTTCGGCGTTGAAGGATCGATCAACAAAGGACTTTCAGTACCGTTAatacga > 1:970456/1‑70 (MQ=255) ccaagccaaCCAAAAGAAGTTCGGCGTTGAAGGATCGATCAACAAAGGACTTTCAGTACCGTTAatacga > 1:3765700/1‑70 (MQ=255) ccaagccaaCCAAAAGAAGTTCGGCGTTGAAGGATCGATCAACAAAGGACTTTCAGTACCGTTAatacga > 1:564398/1‑70 (MQ=255) ccaagccaaCCAAAAGAAGTTCGGCGTTGAAGGATCGATCAACAAAGGACTTTCAGTACCGTTAatac > 1:2129680/1‑68 (MQ=255) ccaagccaaCCAAAAGAAGTTCCGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAatacga > 1:3049236/1‑70 (MQ=255) gccaaCCAAAAGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGt > 1:3513287/1‑71 (MQ=255) gccaaCCAAAAGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGt > 1:1514534/1‑71 (MQ=255) gccaaCCAAAAGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGt > 1:1616160/1‑71 (MQ=255) ccaaccaaAAGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGtt < 1:3809567/71‑1 (MQ=255) ccaaccaaAAGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGtt < 1:3215718/71‑1 (MQ=255) ccaaccaaAAGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGtt < 1:1763087/71‑1 (MQ=255) ccaaccaaAAGAAGTTCGGCGTTGAAGGATCGATCAACAAAGGACTTTCAGTACCGTTAATACGATACGtt < 1:1235436/71‑1 (MQ=255) caaccaaAAGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGtt < 1:3676346/70‑1 (MQ=255) accaaAAGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTgg > 1:2843417/1‑70 (MQ=255) accaaAAGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTgg > 1:2620034/1‑70 (MQ=255) accaaAAGAAGTTCGGCGTTGAAGGATCGATCAACAAAGGACTTTc < 1:3903347/46‑1 (MQ=255) accaaAAGAAGTTCGGCGTTGAAGGATCGATCAACAAAGGACTTTc < 1:347217/46‑1 (MQ=255) accaaAAGAAGTTCGGCGTTGAAGGATCGATCAACAAAGGACTTTc < 1:3815085/46‑1 (MQ=255) accaaAAGAAGTTCGGCGTTGAAGGATAGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTgg > 1:2833146/1‑70 (MQ=255) ccaaAAGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCa < 1:65421/71‑1 (MQ=255) aaaaGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTa > 1:2741290/1‑47 (MQ=255) aaaaGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTa > 1:34854/1‑47 (MQ=255) aaaaGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTa > 1:865586/1‑47 (MQ=255) aaaaGAAGTTCGGCGTTGAAGGATCGGT‑AACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATc > 1:734143/1‑70 (MQ=255) aaaGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAg < 1:1948794/44‑1 (MQ=255) aaaGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAatac < 1:4167901/56‑1 (MQ=255) aaaGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAatac < 1:1953133/56‑1 (MQ=255) aaaGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAatac < 1:1012201/56‑1 (MQ=255) agaagTTCGGCGTTGAAGGATCGATCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCaa > 1:3602988/1‑70 (MQ=255) agaagTTCGGCGTTGAAGGATCGATCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCaa > 1:606638/1‑70 (MQ=255) aagTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCaa > 1:1068271/1‑68 (MQ=255) agTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCaa > 1:1517602/1‑67 (MQ=255) ggCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAGATATTg < 1:94823/71‑1 (MQ=255) ggCGTTGAAGGATCGATCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAGATATTg < 1:2198242/71‑1 (MQ=255) gCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAGATATTGc < 1:1374227/71‑1 (MQ=255) gCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAGATATTGc < 1:900530/71‑1 (MQ=255) gCGTTGAAGGATCGATCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAGATATTg > 1:2172065/1‑70 (MQ=255) gCGTTGAAGGATCGATCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAGATATTGc < 1:1411492/71‑1 (MQ=255) ttGAAGGATCGATCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTgtc < 1:2996495/53‑3 (MQ=255) ttGAAGGATCGATCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGc < 1:1427184/53‑1 (MQ=255) ttGAAGGATCGATCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCaa > 1:746234/1‑58 (MQ=255) gAAGGATCGATCAACAAAGGACTTTCAGTACCGtt < 1:3834884/35‑1 (MQ=255) gAAGGATCGATCAACAAAGGACTTTCAGTACCGtt < 1:1915393/35‑1 (MQ=255) gAAGGATCGATCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCAt > 1:2873875/1‑53 (MQ=255) gAAGGATCGATCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCAt > 1:804589/1‑53 (MQ=255) gAAGGATCGATCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCAt > 1:2059273/1‑53 (MQ=255) gAAGGATCGATCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCAt > 1:3747364/1‑53 (MQ=255) gAAGGATCGATCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGCCAt > 1:3369507/1‑53 (MQ=255) aGGATCGATCAACAAAGGACTTTCAGTACCGTTaa < 1:1739578/35‑1 (MQ=255) aGGATCGATCAACAAAGGACTTTCAGTACCGTTaa < 1:1292618/35‑1 (MQ=255) gatcgatcAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCaa > 1:2451054/1‑52 (MQ=255) gatcgatcAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCaa > 1:3862095/1‑52 (MQ=255) gatcgatcAACAAAGGACTTTCAGTAACGTTAATACGATACGTTGGCATCaa > 1:689085/1‑52 (MQ=255) tCGATCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAGata < 1:1272965/56‑1 (MQ=255) tCGATCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGCCATCAACAGata < 1:2387377/56‑1 (MQ=39) gTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACa < 1:1428253/49‑1 (MQ=255) gTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACa < 1:4071531/49‑1 (MQ=255) gTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACa < 1:3894173/49‑1 (MQ=255) gTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACa < 1:1071645/49‑1 (MQ=255) gTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAGat < 1:2200761/52‑1 (MQ=255) | TTTTTTGCTCATAAAATCACGCGCCTGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAGATATTGCAGTGG > minE/2321634‑2321759 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |