| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,348,624 | T→G | 9.8% | intergenic (+144/+237) | yihA → / ← polA | GTP‑binding protein/fused DNA polymerase I 5'‑>3' exonuclease, 3'‑>5' polymerase and 3'‑>5' exonuclease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,348,624 | 0 | T | G | 9.8% | 117.5 / 9.3 | 51 | intergenic (+144/+237) | yihA/polA | GTP‑binding protein/fused DNA polymerase I 5'‑>3' exonuclease, 3'‑>5' polymerase and 3'‑>5' exonuclease |
| Reads supporting (aligned to +/- strand): ref base T (27/19); new base G (3/2); total (30/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
GGCCTGATAAACATAGCGCATCAGGCATTACGGATCTTTTCTTTCGCCCAATAAAAAACGCCCCAGTCATTACTGACTGGGGCGGCTAAAATATTCAGCCAAATCCGATTACGTGAAGTAAAAGGTCTGAAAGATAGAAC > minE/2348555‑2348694 | ggCCTTATAAACATAGCGCATCTGGCATTAGGGATCTTTTCTTTCGCCCAATAAAAAACGCCCCAGTCAt < 1:2900314/70‑1 (MQ=255)ggCCTGATAAACATAGCGCATCAGGCATTACGGATCTTTTCTTTCGCCCAATAAAAAACGCCCCAGTCAt < 1:910681/70‑1 (MQ=255)ggCCTGATAAACATAGCGCATCAGGCATTACGGATCTTTTCTTTCGCCCAATAAAAAACGCCCCAGTCAt < 1:819903/70‑1 (MQ=255)ggCCTGATAAACATAGCGCATCAGGCATTACGGATCTTTTCTTTCGCCCAATAAAAAACGCCCCAGTCAt < 1:453345/70‑1 (MQ=255)ggCCTGATAAACATAGCGCATCAGGCATTACGGATCTTTTCTTTCGCCCAATAAAAAACGCCCCAGTCAt < 1:2176177/70‑1 (MQ=255)ggCCTGATAAACATAGCGCATCAGGCATTACGGATCTTTTCTTTCGCCCAATAAAAAACGCCCCAGTCAt < 1:2098500/70‑1 (MQ=255)ggCCTGATAAACATAGCGCATCAGGCATTACGGATCTTTTCTTTCGCCCAATAAAAAACGCCCCAGTCAt < 1:2060483/70‑1 (MQ=255)ggCCTGATAAACATAGCGCATCAGGCATTACGGATCTTTTCTTTCGCCCAATAAAAAACGCCCCAGTCAt < 1:1808255/70‑1 (MQ=255) gCCTGATAAACATAGCGCATCAGGCATTACGGATCTTTTCTTTCGCCCAATAAAAAACGCCCCAGTCAt < 1:900488/69‑1 (MQ=255) cTGATAAACATAGCGCATCAGGCATTACGGATCTTTTCTTTCGCCCAATAAAAAACGCCCCAGTCATTact < 1:2703571/71‑1 (MQ=255) cTGATAAACATAGCGCATCAGGCATTACGGATCTTTTCTTTCGCCCAATAAAAAACGCCCCAGTCATTact < 1:3536863/71‑1 (MQ=255) cTGATAAACATAGCGCATCAGGCATTACGGATCTTTTCTTTCGCCCAATAAAAAACGCCCCAGTCATTact < 1:2601438/71‑1 (MQ=255) cTGATAAACATAGCGCATCAGGCATTACGGATCTTTTCTTTCGCCCAATAAAAAACGCCCCAGTCATTact < 1:1841222/71‑1 (MQ=255) gATAAACATAGCGCATCAGGCATTACGGATCTTTTCTTTCGCCCAATAAAAAACGCCCCAGTCAt < 1:16728/65‑1 (MQ=255) aTAAACATAGCGCATCAGGCATTACGGATCTTTTCTTTCGCCCAATAAAAAACGCCCCAGTCATTactg < 1:2250018/69‑1 (MQ=255) tAAACATAGCGCATCAGGCATTACGGATCTTTTCTTTCGCCCAATAAAAAACGCCCCAGTCATTactgact > 1:3589186/1‑71 (MQ=255) tAAACATAGCGCATCAGGCATTACGGATCTTTTCTTTCGCCCAATAAAAAACGCCCCAGTCATTactgact > 1:2792725/1‑71 (MQ=255) tAAACATAGCGCATCAGGCATTACGGATCTTTTCTTTCGCCCAATAAAAAACGCCCCAGTCATTactgact > 1:688833/1‑71 (MQ=255) tAAACATAGCGCATCAGGCATTACGGATCTTTTCTTTCGCCCAATAAAAAACGCCCCAGTCATTactga < 1:3310633/69‑1 (MQ=255) aTAGCGCATCAGGCATTACGGATCTTTTCTTTCGCCCAATAAAAAACGCCCCAGTCATTACTGACTGGggc < 1:2966399/71‑1 (MQ=255) aGCGCATCAGGCATTACGGATCTTTTCTTTCGCCCAATAAAAAACGCCCCAGTCATTACTGACTGGggcgg > 1:416496/1‑71 (MQ=255) cgcATCAGGCATTACGGATCTTTTCTTTCGCCCAATAAAAAACGCCCCAGTCATTact < 1:1542244/58‑1 (MQ=255) gcATCAGGCATTACGGATCTTTTCTTTCGCCCAATAAAAAACGCCCCAGTCATTACTGACTGGGGCGGCt > 1:1299640/1‑70 (MQ=255) aGGCATTACGGATCTTTTCTTTCGCCCAATAAAACACGCCCCAGTCATTact < 1:3108506/52‑1 (MQ=255) aGGCATTACGGATCTTTTCTTTCGCCCAATAAAAAACGCCCCAGTCATTACTGACTGGGGCGGCTAAAata > 1:3022593/1‑71 (MQ=255) aTTACGGATCTTTTCTTTCGCCCAATAAAAAACGCCCCAGTCATTACTGACTGGGGCGGCTAAAATAtt > 1:1644519/1‑69 (MQ=255) aTTACGGATCTTTTCTTTCGCCCAATAAAAAACGCCCCAGTCATTACTGACTGGGGCGGCTAAAATAtt > 1:4226933/1‑69 (MQ=255) aCGGATCTTTTCTTTCGCCCAATAAAAAACGCCCCAGTCATTactgact > 1:1353989/1‑49 (MQ=255) gtaTCTTTTCTTTCGCCCAATAAAAAACGCCCCAGTCATTACTGACTGGGGCGGCTAAAata < 1:129946/60‑1 (MQ=255) gATCTTTTCTTTCGCCCAATAAAAAACGCCCCAGTCAt < 1:3899581/38‑1 (MQ=255) cttttctttCGCCCAATAAAAAACGCCCCAGTCATTactg < 1:1459036/40‑1 (MQ=255) tttctttcGCCCAATAAAAAACGCCCCAGTCATTa > 1:313852/1‑35 (MQ=255) tttctttcGCCCAATAAAAAACGCCCCAGTCATTa > 1:2536534/1‑35 (MQ=255) tttctttcGCCCAATAAAAAACGCCCCAGTCATTa > 1:1399736/1‑35 (MQ=255) tctttcGCCCAATAAAAAACGCCCCAGTCATTACTGACTGGGGCGGCTAAAATATTCAGCCAAATCCGAt < 1:745214/70‑1 (MQ=255) ctttcGCCCAATAAAAAACGCCCCAGTCATTACTGACTGGGGCGGCTaaaa > 1:2624203/1‑51 (MQ=255) ctttcGCCCAATAAAAAACGCCCCAGTCATTACTGACTGGGGCGGCTaaaa > 1:2117871/1‑51 (MQ=255) gCCCAATAAAAAACGCCCCAGTCATTACTGACTGGGGCGGCTAAAATATTCAGCCAAATCCGATTACGTg < 1:158272/70‑1 (MQ=255) ccAATAAAAAACGCCCCAGTCATTACTGACTGGGGCGGCTAAAATATTCAGCCAAATCCGATTACTTg < 1:1388208/68‑1 (MQ=255) aTAAAAAACGCCCCAGTCATTACTGACTGGGGCGGCTAAAATATTCAGCCAAATCCGATTACGTGAAGTaa < 1:1222784/71‑1 (MQ=255) tAAAAAACGCCCCAGTCATTACTGACTGGGGCGGCTAAAATATTCAGCCAAATCCGATTACGTGAAGTaa > 1:2610222/1‑70 (MQ=255) aaaaaCGCCCCAGTCATTACTGACTGGGGCGGCTAAAATATTCAGCCAAATCCGATTACGTGAAGTAAAAg > 1:3568690/1‑71 (MQ=255) aaaCGCCCCAGTCATTACTGACTGGGGCGGCTAAAATATTCAGCCAAATCCGATTACGTGAAGTAAAAGGt < 1:4187158/71‑1 (MQ=255) aaCGCCCCAGTCATTACTGACTGGGGCGGCTAAAATATTCAGCCAAATCCGATTACGTGAAGTAAAAGGTc < 1:1235500/71‑1 (MQ=255) cccAGTCATTACTGACTGGGGCGGCTAAAATATTCAGCCAAATCCGATTACGTGAAGTAAAAGGTCTGaaa < 1:1025740/71‑1 (MQ=255) cccAGTCAGTAATGACTGGGGCGGCTAAAATATTCAGCCAAATCCGATTACGTGAAGTAAAAGGTCTGaaa < 1:3235329/71‑1 (MQ=255) cccAGTCAGTAATGACTGGGGCGGCTAAAATATTCAGCCAAATCCGATTACGTGAAGTAAAAGGTCTGaaa < 1:4096043/71‑1 (MQ=255) ccAGTCATTACTGACTGGGGCGGCTAAAATATTCAGCCAAATCCGATTACGTGAAGTAAAAGGTCTGAAAg < 1:269266/71‑1 (MQ=255) ccAGTCAGTAATGACTGGGGCGGCTAAAATATTCCGCCAAATCCGATTACGGGAAGTAAAAGGTCTGa > 1:1434107/1‑68 (MQ=37) ccAGTCAGTAATGACTGGGGCGGCTAAAATATTCAGCCAAATCCGATTACGTGAAGTAAAAGGTCTGa > 1:1428931/1‑68 (MQ=255) ccAGTCAGTAATGACTGGGGCGGCTAAAATATTCAGCCAAATCCGATTACGTGAAGTAAAAGGTCTGa > 1:1293185/1‑68 (MQ=255) cagtcagtAATGACTGGGGCGGCTAAAATATTCAGCCAAATCCGATTACGTGAAGTAAAAGGTCt > 1:3712635/1‑65 (MQ=255) cagtcagtAATGACTGGGGCGGCTAAAATATTCAGCCAAATCCGATTACGTGAAGTAAAAGGTCTg > 1:3717446/1‑66 (MQ=255) cagtcagtAATGACTGGGGCGGCTAAAATATTCAGCCAAATCCGATTACGTGAAGTAAAAGGTCTGa > 1:1476302/1‑67 (MQ=255) cagtcagtAATGACTGGGGCGGCTAAAATATTCAGCCAAATCCGATTACGTGAAGTAAAAGGTCTGa > 1:89177/1‑67 (MQ=255) cAGTCATTACTGACTGGGGCGGCTAAAATATTCAGCCAAATCCGATTACGTGTAGTAAAAGGTCTGa > 1:3674740/1‑67 (MQ=255) cAGTCATTACTGACTGGGGCGGCTAAAATATTCAGCCAAATCCGATTACGTGAAGTAAAAGGTCTg > 1:2432140/1‑66 (MQ=255) cAGTCATTACTGACTGGGGCGGCTAAAATATTCAGCCAAATCCGATTACGTGAAGTAAAAGGTCTGa > 1:3669804/1‑67 (MQ=255) cAGTCATTACTGACTGGGGCGGCTAAAATATTCAGCCAAATCCGATTACGTGAAGTAAAAGGTCTGa > 1:3156637/1‑67 (MQ=255) gTCATTACTGACTGGGGCGGCTAAAATATTCAGCCAAATCCGATTACGTGAAGTaaaa > 1:199846/1‑58 (MQ=255) gTCATTACTGACTGGGGCGGCTAAAATATTCAGCCAAATCCGATTACGTGAAGTaaaa > 1:570585/1‑58 (MQ=255) aTTACTGACTGTGGCGGCTAAAATATTCAGCCAAATCCGATTACGTGAAGTAAAAGGTCTGAAAGa > 1:3824692/1‑66 (MQ=255) aTTACTGACTGGGGCGGCTAAAATATTCAGCCAAATCCGATTACGTGAAGTAAAAGGTCTGAAAGa > 1:188879/1‑66 (MQ=255) aTTACTGACTGGGGCGGCTAAAATATTCAGCCAAATCCGATTACGTGAAGTAAAAGGTCTGAAAGa > 1:3551007/1‑66 (MQ=255) aTTACTGACTGGGGCGGCTAAAATATTCAGCCAAATCCGATTACGTGAAGTAAAAGGTCTGAAAGa > 1:2576747/1‑66 (MQ=255) aTTACTGACTGGGGCGGCTAAAATATTCAGCCAAATCCGATTACGTGAAGTAAAAGGTCTGAAAGATAGaa > 1:59545/1‑71 (MQ=255) ttACTGACTGGGGCGGCTAAAATATTCAGCCAAATCCGATTACGTg < 1:108294/46‑1 (MQ=255) ttACTGACTGGGGCGGCTAAAATATTCAGCCAAATCCGATTACGTGAAGTAAAAGGTCTGAAAGATAGAAc < 1:850766/71‑1 (MQ=255) | GGCCTGATAAACATAGCGCATCAGGCATTACGGATCTTTTCTTTCGCCCAATAAAAAACGCCCCAGTCATTACTGACTGGGGCGGCTAAAATATTCAGCCAAATCCGATTACGTGAAGTAAAAGGTCTGAAAGATAGAAC > minE/2348555‑2348694 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |