| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,377,802 | A→T | 31.7% | Y196N (TAC→AAC) | ubiB ← | 2‑octaprenylphenol hydroxylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,377,802 | 0 | A | T | 31.7% | 86.9 / 53.5 | 63 | Y196N (TAC→AAC) | ubiB | 2‑octaprenylphenol hydroxylase |
| Reads supporting (aligned to +/- strand): ref base A (28/15); new base T (14/6); total (42/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.80e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01 | |||||||||||
TGCGCCGAAGCTGAATGGCGTTGGCAGATTCCCGCAGCAAATTCAGTTCATCAATCAATGTCTTTTCGTACTCGCGCACCACTTCGGTTGGGCGCAGACGGCGACCATCCGGCAGCAAACGCGGCACCCAGCGAGCCA > minE/2377733‑2377870 | tGCGCCGAAGCTGAATGGCGTTGGCAGATTCCCGCAGCAAATTCAGTTCATCAATCAATGTCTTTTCGTAc > 1:1636824/1‑71 (MQ=255)tGCGCCGAAGCTGAATGGCGTTGGCAGATTCCCGCAGCAAATTCAGTTCATCAATCAATGTCTTTTCGTAc > 1:3014042/1‑71 (MQ=255) gAAGCTGAATGGCGTTGGCAGATTCCCGCAGCAAATTCAGTTCATCAATCAATGTCTTTTCGTTCTCgcgc < 1:1699272/71‑1 (MQ=255) gAATGGCGTTGGCAGATTCCCGCAGCAAATTCAGTTCATCAATCAATGTCTTTTCGTACt > 1:3772917/1‑60 (MQ=255) gAATGGCGTTGGCAGATTCCCGCAGCAAATTCAGTTCATCAATCAATGTCTTTTCGTACTCGCGCACCACt > 1:1901337/1‑71 (MQ=255) ggCAGATTCCCGCAGCAAATTCAGTTCATCAATCAATGTCTTTTCGTACTCGCGCACCACTTCGGTTGGgc > 1:366813/1‑71 (MQ=255) cAGTTTCCCGCAGCAAATTCAGTTCATCAATCAATGTCTTTTCGTACTCGCGCACCACTTCGGTTGGgcgc < 1:2501737/71‑1 (MQ=255) cAGATTCCCGCAGCAAATTCAGTTCATCAATCAATGTCTTTTCGTACTcgc < 1:3724608/51‑1 (MQ=255) cAGATTCCCGCAGCAAATTCAGTTCATCAATCAATGTCTTTTCGTACTcgc < 1:3547282/51‑1 (MQ=255) cAGATTCCCGCAGCAAATTCAGTTCATCAATCAATGTCTTTTCGTACTCGCGCACCACTTCGGTTGGgcg > 1:3952141/1‑70 (MQ=255) cAGATTCCCGCAGCAAATTCAGTTCATCAATCAATGTCTTTTCGTACTCGCGCACCACTTCGGTTGGgcg > 1:72576/1‑70 (MQ=255) aGATTCCCGCAGCAAATTCAGTTCATCAATCAATGTCTTTTCGTACTCGCGCACCACTTCGGTTGGTCGCa < 1:2847909/71‑1 (MQ=255) aTTCCCGCAGCAAATTCAGTTCATCAATCAATGTCTTTTCGTTCTCGCGCACCACTTCGGTTGGGCGCAGa > 1:1647516/1‑71 (MQ=255) ttCCCGCAGCAAATTCAGTTCATCAATCAATGTCTTTTCGTTCTCGCGCACCACTTCGGTTGGGCGCAGAc > 1:3018824/1‑71 (MQ=255) cGCAGCAAATTCAGTTCATCAATCAATGTCTTTTCGTACTCGCGCACCACTTCGGTTGGGCGCAGACGGCg < 1:2370752/71‑1 (MQ=255) gcagcaAATTCAGTTCATCAATCAATGTCTTTTCGTTCTCGCGCACCACTTCGGTTGGgcgaa > 1:1146641/1‑61 (MQ=255) gcagcaAATTCAGTTCATCAATCAATGTCTTTTCGTTCTCGCGCACCACTTCGGTTGGGCGCa > 1:480996/1‑63 (MQ=255) gcagcaAATTCAGTTCATCAATCAATGTCTTTTCGTTCTCGCGCACCACTTCGGTTGGGCGCa > 1:191021/1‑63 (MQ=255) gcagcaAATTCAGTTCATCAATCAATGTCTTTTCGTTCTCGCGCACCACTTCGGTTGGGCGCa > 1:4255863/1‑63 (MQ=255) gcagcaAATTCAGTTCATCAATCAATGTCTTTTCGTTCTCGCGCACCACTTCGGTTGGGCGCa > 1:1845807/1‑63 (MQ=255) gcagcaAATTCAGTTCATCAATCAATGTCTTTTCGTTCTCGCGCACCACTTCGGTTGGGCGCa > 1:1887941/1‑63 (MQ=255) gcagcaAATTCAGTTCATCAATCAATGTCTTTTCGTTCTCGCGCACCACTTCGGTTGGGCGCa > 1:1609551/1‑63 (MQ=255) ttCAGTTCATCAATCAATGTCTTTTCGTACTCgcgc < 1:2036166/36‑1 (MQ=255) ttCAGTTCATCAATCAATGTCTTTTCGTACTCgcgc < 1:1279196/36‑1 (MQ=255) ttCAGTTCATCAATCAATGTCTTTTCGTACTCgcgc < 1:3197574/36‑1 (MQ=255) ttCAGTTCATCAATCAATGTCTTTTCGTACTCGCTCACCACTTc < 1:2057383/44‑1 (MQ=255) ttCAGTTCATCAATCAATGTCTTTTCGTACTCGCGCACCACTTc < 1:3852556/44‑1 (MQ=255) ttCAGTTCATCAATCAATGTCTTTTCGTACTCGCGCACCACTTc < 1:360779/44‑1 (MQ=255) ttCAGTTCATCAATCAATGTCTTTTCGTACTCGCGCACCACTTc < 1:744470/44‑1 (MQ=255) cAGTTCATCAATCAATGTCTTTTCGTACTCGCGcac > 1:3342758/1‑36 (MQ=255) cAGTTCATCAATCAATGTCTTTTCGTACTCGCGcac > 1:287081/1‑36 (MQ=255) catcaATCAATGTCTTTTCGTTCTCGCGCACCACTTCGGTTGGGCGCAGACGGCGACCATCCGGCAGCaaa > 1:3260319/1‑71 (MQ=255) catcaATCAATGTCTTTTCGTTCTCGCGCACCACTTCGGTTGGGCGCAGACGGCGACCATCCGGCAGCaaa > 1:1963845/1‑71 (MQ=255) catcaATCAATGTCTTTTCGTACTCGCGCACCACTTCGGt > 1:87272/1‑40 (MQ=255) catcaATCAATGTCTTTTCGTACTCGCGCACCACTTCGGt > 1:1154375/1‑40 (MQ=255) catcaATCAATGTCTTTTCGTACTCGCGCACCACTTCGGt > 1:1492160/1‑40 (MQ=255) catcaATCAATGTCTTTTCGTACTCGCGCACCACTTCGGt > 1:2978134/1‑40 (MQ=255) catcaATCAATGTCTTTTCGTACTCGCGCACCACTTCGGt > 1:1750698/1‑40 (MQ=255) catcaATCAATGTCTTTTCGTACTCGCGCACCACTTCGGTTGGGCGCAGACGGCGACCATCCGGCAGCaaa > 1:293797/1‑71 (MQ=255) tcaatcaaTGTCTTTTCGTTCTCGCGCACCACTTCGGTTGGGCGCAGACGGCGAc < 1:285951/55‑1 (MQ=255) tcaatcaaTGTCTTTTCGTTCTCGCGCACCACTTCGGTTGGGCGCAGACGGCGAc < 1:1088162/55‑1 (MQ=255) tcaatcaaTGTCTTTTCGTTCTCGCGCACCACTTCGGTTGGGCGCAGACGGCGACCATCCGGCAGCAAAcg < 1:3676359/71‑1 (MQ=255) tcaatcaaTGTCTTTTCGTACTCGCGCACCACTTCGGTTgg > 1:2123501/1‑41 (MQ=255) tcaatcaaTGTCTTTTCGTACTCGCGCACCACTTCGGTTgg > 1:3886271/1‑41 (MQ=255) tcaatcaaTGTCTTTTCGTACTCGCGCACCACTTCGGTTgg > 1:1359778/1‑41 (MQ=255) tcaatcaaTGTCTTTTCGTACTCGCGCACCACTTCGGTTgg > 1:2571278/1‑41 (MQ=255) aatcaatGTCTTTTCGTACTCGCGCACCACTTCGGTTAGGCGCAGACGGCGACCATCCGGCAGCAAAcgc < 1:1052298/70‑1 (MQ=255) aatGTCTTTTCGTTCTCGCGCACCACTTCGGTTGGGCGCAGACGGCGACCATCCGGCAGCAAACGCGGCa < 1:2293387/70‑1 (MQ=255) aatGTCTTTTCGTTCTCGCGCACCACTTCGGTTGGGCGCAGACGGCGACCATCCGGCAGCAAACGCGGCAc > 1:1811956/1‑71 (MQ=255) aatGTCTTTTCGTTCTCGCGCACCACTTCGGTTGGGCGCAGACGGCGACCATCCGGCAGCAAACGCGGCAc > 1:854998/1‑71 (MQ=255) aatGTCTTTTCGTTCTCGCGCACCACTTCGGTTGGGCGCAGACGGCGACCATCCGGCAGCAAACGCGGCAc > 1:794761/1‑71 (MQ=255) aatGTCTTTTCGTACTCGCGCACCACTTCGGTTGGGCGCa < 1:1203776/40‑1 (MQ=255) aatGTCTTTTCGTACTCGCGCACCACTTCGGTTGGGCGCAGAc > 1:853775/1‑43 (MQ=255) aatGTCTTTTCGTACTCGCGCACCACTTCGGTTGGGCGCAGAc > 1:2745484/1‑43 (MQ=255) aatGTCTTTTCGTACTCGCGCACCACTTCGGTTGGGCGCAGAc > 1:3110356/1‑43 (MQ=255) tCTTTTCGTACTCGCGCACCACTTCGGTTGGgcgc > 1:2781704/1‑35 (MQ=255) tCTTTTCGTACTCGCGCACCACTTCGGTTGGGCGCa > 1:3306160/1‑36 (MQ=255) tCTTTTCGTACTCGCGCACCACTTCGGTTGGGCGCa > 1:2222571/1‑36 (MQ=255) tCTTTTCGTACTCGCGCACCACTTCGGTTGGGCGCAGACGGCGACCATCCGGCAGCAAACGCGGCACCCAg > 1:1112765/1‑71 (MQ=255) tCTTTTCGTACTCGCGCACCACTTCGGTTGGGCGCAGACGGCGACCATCCGGCAGCAAACGCGGCACCCAg > 1:1078125/1‑71 (MQ=255) tCTTTTAGTACTCGCGCAACACTTCGGTTGGGCGCAGACGGCGACCATCCGGCAGCAAACGCGGCACCCAg > 1:1504921/1‑71 (MQ=255) ttCGTTCTCGCGCACCACTTCGGTTGGGCGCAGACGGCGACCATCCGGCAGCAAACGCGGCACCCAGCGa < 1:3554462/70‑1 (MQ=255) gTACTCGCGCACCACTTCGGTTGGGCGCAGACGGCGACCATCCGGCAGCAAACGCTGCACCCAGCGAGCCa < 1:286242/71‑1 (MQ=255) | TGCGCCGAAGCTGAATGGCGTTGGCAGATTCCCGCAGCAAATTCAGTTCATCAATCAATGTCTTTTCGTACTCGCGCACCACTTCGGTTGGGCGCAGACGGCGACCATCCGGCAGCAAACGCGGCACCCAGCGAGCCA > minE/2377733‑2377870 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |