Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 297,667 C→T 32.2% R110C (CGT→TGT)  mdlA → fused predicted multidrug transporter subunits and ATP‑binding components of ABC superfamily

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE297,6670CT32.2% 84.1 / 52.4 62R110C (CGT→TGT) mdlAfused predicted multidrug transporter subunits and ATP‑binding components of ABC superfamily
Reads supporting (aligned to +/- strand):  ref base C (21/21);  new base T (10/10);  total (31/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AACTGGCTGTTGAACTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCG  >  minE/297599‑297733
                                                                    |                                                                  
aaCTGGCTGTTGAACTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTTCCTgtgt                                                                  <  1:3530088/71‑1 (MQ=255)
aaCTGGCTGTTGAACTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTgtgt                                                                  <  1:3309645/71‑1 (MQ=255)
  cTGGCTGTTGAACTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGTGTCa                                                                <  1:3318233/71‑1 (MQ=255)
  cTGGCTGTTGAACTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGTGTCa                                                                <  1:3737919/71‑1 (MQ=255)
    ggCTGTTGAACTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATc                                                              >  1:2608729/1‑71 (MQ=255)
     gCTGTTGAACTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGTGTCAt                                                               <  1:1521606/69‑1 (MQ=255)
     gCTGTTGAACTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCAt                                                               <  1:796335/69‑1 (MQ=255)
     gCTGTTGAACTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCAt                                                               <  1:729798/69‑1 (MQ=255)
     gCTGTTGAACTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCAt                                                               <  1:1902152/69‑1 (MQ=255)
         ttGAACTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGTGTCATCGCAcc                                                         >  1:3234118/1‑71 (MQ=255)
          tGAACTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCg                                                        >  1:2860085/1‑71 (MQ=255)
           gAACTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATc                                                              >  1:35783/1‑64 (MQ=255)
             aCTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGTGTCATCGCACCGGt                                                      <  1:1099377/70‑1 (MQ=255)
             aCTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGGCATCGCACCGGTg                                                     <  1:24127/71‑1 (MQ=255)
                gCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGAcc                                                  >  1:1625714/1‑71 (MQ=255)
                gCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGAcc                                                  >  1:1029266/1‑71 (MQ=255)
                gCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGAcc                                                  >  1:2119388/1‑71 (MQ=255)
                   tGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCa                                               >  1:4063121/1‑71 (MQ=255)
                    gAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTc                                                                 <  1:2914836/52‑1 (MQ=255)
                    gAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTc                                                                 <  1:275985/52‑1 (MQ=255)
                    gAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTc                                                                 <  1:3433990/52‑1 (MQ=255)
                     aaGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGTGTCa                                                                <  1:1850009/52‑1 (MQ=255)
                         ttattaCCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGTGTCATCGCACCGGTGACCTCATGGCTc                                         >  1:2308285/1‑71 (MQ=255)
                         ttattaCCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGTGTCATCGCACCGGTGACCTCATGGCTc                                         >  1:814/1‑71 (MQ=255)
                         ttattaCCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCgg                                                       >  1:716695/1‑57 (MQ=255)
                         ttattaCCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCgg                                                       >  1:2016899/1‑57 (MQ=255)
                          tattaCCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGTGTCATCGCACCGGTGACCTCATGGCTCg                                        >  1:2704683/1‑71 (MQ=255)
                          tattaCCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGTGTCATCGCACCGGTGACCTCATGGCTCg                                        >  1:2709397/1‑71 (MQ=255)
                          tattaCCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGTGTCATCGCACCGGTGACCTCATGGCTCg                                        >  1:1836870/1‑71 (MQ=255)
                          tattaCCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGTGTCATCGCACCGGTGACCTCATGGCTCg                                        >  1:3805879/1‑71 (MQ=255)
                           attaCCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCAcc                                                         >  1:280276/1‑53 (MQ=255)
                           attaCCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCg                                        >  1:1566534/1‑70 (MQ=255)
                           attaCCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGt                                       >  1:3417954/1‑71 (MQ=255)
                           attaCCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGt                                       >  1:3941390/1‑71 (MQ=255)
                           attaCCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGt                                       >  1:1061224/1‑71 (MQ=255)
                           attaCCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGt                                       >  1:1354176/1‑71 (MQ=255)
                                cGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGt                                                      <  1:3753299/51‑1 (MQ=255)
                                  tCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGTGTCATCGCACCGGTCACCTCATGGCTCGTGCGACCa                                <  1:2123571/71‑1 (MQ=255)
                                    aGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAAt                              <  1:3247724/71‑1 (MQ=255)
                                    aGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAAt                              <  1:2859530/71‑1 (MQ=255)
                                    aGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAAt                              <  1:2843344/71‑1 (MQ=255)
                                      cTAAGCCGGCAGCATCCTGAGTTTTACCTGTGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGa                            <  1:1460537/71‑1 (MQ=255)
                                      cTAAGCCGGCAGCATCCTGAGTTTTACCTGTGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGa                            <  1:1681676/71‑1 (MQ=255)
                                       tAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGa                            >  1:3288258/1‑70 (MQ=255)
                                       tAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGa                            >  1:731168/1‑70 (MQ=255)
                                           ccGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCg                       <  1:794335/71‑1 (MQ=255)
                                           ccGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCg                       <  1:4240719/71‑1 (MQ=255)
                                            cGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGa                      <  1:2051878/71‑1 (MQ=255)
                                            cGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGa                      <  1:1247576/71‑1 (MQ=255)
                                            cGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGa                      <  1:1189841/71‑1 (MQ=255)
                                            cGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGa                      <  1:4084265/71‑1 (MQ=255)
                                            cGGCAGCATCCTGACTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCg                       <  1:4074016/70‑1 (MQ=255)
                                                   aTCCTGAGTTTTACCTGTGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGcgtc               <  1:4191813/71‑1 (MQ=255)
                                                    tCCTGAGTTTTACCTGTGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAAt                              <  1:1322667/55‑1 (MQ=255)
                                                    tCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCg                       <  1:771212/62‑1 (MQ=255)
                                                      cTGAGTTTTACCTGTGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGCCGTCGATcgcg                 >  1:2477594/1‑66 (MQ=255)
                                                      cTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCgtg            >  1:3819879/1‑71 (MQ=255)
                                                         aGTTTTACCTGTGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGttt         >  1:1292530/1‑71 (MQ=255)
                                                         aGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCgtgt           <  1:2918556/69‑1 (MQ=255)
                                                         aGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCgtgt           <  1:800862/69‑1 (MQ=255)
                                                             ttACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTgcc      >  1:2614234/1‑70 (MQ=255)
                                                              tACCTGTGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTgccgc    >  1:3766407/1‑71 (MQ=255)
                                                                   gCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGtt          >  1:3323416/1‑60 (MQ=255)
                                                                   gCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTGccgccg  >  1:98026/1‑68 (MQ=255)
                                                                    |                                                                  
AACTGGCTGTTGAACTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCG  >  minE/297599‑297733

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: