| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,467,238 | G→T | 31.8% | L234L (CTG→CTT) | atpA → | F1 sector of membrane‑bound ATP synthase, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,467,238 | 0 | G | T | 31.8% | 185.6 / 61.8 | 91 | L234L (CTG→CTT) | atpA | F1 sector of membrane‑bound ATP synthase, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base G (31/31); new base T (20/9); total (51/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.14e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01 | |||||||||||
Only 100 of 102 total aligned reads displayed.
ACTGGAAGAGCACGGCGCACTGGCTAACACCATCGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACCGCGGTGAAGA > minE/2467169‑2467300 | aCTGGAAGAGCACGGCGCACTGGCTAACACCATCGTTGTGGTAGCAACCGCGTCTGAATCGGctgcactgc < 1:1457026/71‑1 (MQ=255)aCTGGAAGAGCACGGCGCACTGGCTAACACCATCGTTGTGGTAGCAACCGCGTCTGAATCCGctgcactgc < 1:3763096/71‑1 (MQ=255)aCTGGAAGAGCACGGCGCACTGGCTAACACCATCGTTGTGGTAGCAACCGCGTCTGAATCCGctgcactgc < 1:2078160/71‑1 (MQ=255) gAAGAGCACGGCGCACTGGCTAACACCATCGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTTCAAt < 1:651226/70‑1 (MQ=255) gAAGAGCACGGCGCACTGGCTAACACCATCGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTGCaa < 1:2587196/69‑1 (MQ=255) gAAGAGCACGGCGCACTGGCTAACACCATCGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTGCaa < 1:910515/69‑1 (MQ=255) ggCGCACTGGCTAACACCATCGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTTCAATACCTGGCAcc > 1:841852/1‑71 (MQ=255) gcgcACTGGCTAACACCATCGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTGCAATACCTGGCACCg > 1:1819538/1‑71 (MQ=255) gcgcACTGGCTAACACCATCGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTGCAATACCTGGCACCg > 1:1422778/1‑71 (MQ=255) gcgcACTGGCTAACACCATCGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTGCAATACCTGGCACCg > 1:2071935/1‑71 (MQ=255) cgcACTGGCTAACACCATCGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTTCAATACCTGGCACCg < 1:1645944/70‑1 (MQ=255) cACTGGCTAACACCATCGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTTCAATACCTGGCACCGTAt > 1:1944221/1‑71 (MQ=255) cACTGGCTAACACCATCGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTTCAATACCTGGCACCGTAt > 1:324364/1‑71 (MQ=255) cACTGGCTAACACCATCGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTTCAATACCTGGCACCGTAt > 1:329395/1‑71 (MQ=255) cTGGCTAACACCATCGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTGCAATACCt < 1:346826/59‑1 (MQ=255) cTGGCTAACACCATCGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTGCAATACCt < 1:1404249/59‑1 (MQ=255) cTGGCTAACACCATCGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTGCAATACCt < 1:1751288/59‑1 (MQ=255) cTGGCTAACACCATCGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTGCAATACCt < 1:521817/59‑1 (MQ=255) ggCTAACACCATCGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCg > 1:840124/1‑71 (MQ=255) ggCTAACACCATCGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCg > 1:2012809/1‑71 (MQ=255) ggCTAACACCATCGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCg > 1:239618/1‑71 (MQ=255) ggCTAACACCATCGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCg > 1:140612/1‑71 (MQ=255) gCTAACACCATCGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCgg > 1:4116786/1‑71 (MQ=255) tAACACCATCGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTTCAATACCTGGCACCGTATGCCGGtt > 1:3476065/1‑71 (MQ=255) acacCATCGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTTCAATACCTGGCACCGTATGCCGGTTgc > 1:2857749/1‑71 (MQ=255) acacCATCGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTTCAATACCTGGCACCGTATGCCGGTTgc > 1:2687832/1‑71 (MQ=255) acacCATCGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTTCAATACCTGGCACCGTATGCCGGTTgc > 1:1974123/1‑71 (MQ=255) acacCATCGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTTCAATACCTGGCACCGTATGCCGGTTgc > 1:3592584/1‑71 (MQ=255) acacCATCGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTTCAATACCTGGCACCGTATGCCGGTTgc > 1:2778842/1‑71 (MQ=255) acacCATCGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTTCAATACCTGGCACCGTATGCCGGTTgc > 1:3307786/1‑71 (MQ=255) acacCATCGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTTCAATACCTGGCACCGTATGCCGGTTgc > 1:1350256/1‑71 (MQ=255) acCATCGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTgcgc < 1:1891614/71‑1 (MQ=255) acCATCGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTgcgc < 1:4183529/71‑1 (MQ=255) acCATCGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTgcgc < 1:1017322/71‑1 (MQ=255) acCATCGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTgcgc < 1:1539419/71‑1 (MQ=255) acCATCGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTgcgc < 1:841429/71‑1 (MQ=255) acCATCGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTgcgc < 1:891659/71‑1 (MQ=255) acCATCGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTgcgc < 1:875959/71‑1 (MQ=255) acCATCGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTgcg > 1:1942187/1‑70 (MQ=255) ccATCGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTTCAATACCTGGCACCGTATGCCGGTTGCGCa < 1:3014775/71‑1 (MQ=255) aTCGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTGCGCAAt < 1:388076/71‑1 (MQ=255) aTCGTAGTGGTAGCAACCGCGTCTGAATCCGCTGCACTTCAAAACCTGGCACCGTATGCCGGTTGCGCAAt > 1:1332785/1‑71 (MQ=255) tCGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTTCAATACCTGGCACCGTATGCCGGTTGCGCAATg < 1:2095779/71‑1 (MQ=255) tCGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTTCAATACCTGGCACCGTATGCCGGTTGCGCAATg < 1:576098/71‑1 (MQ=255) tCGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTGCAATACCTGGCACCg < 1:1245687/53‑1 (MQ=255) gTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTTCAATAcc > 1:1111477/1‑43 (MQ=255) gTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTGCGCAATgg < 1:1024242/70‑1 (MQ=255) tgtgGTAGCAACCGCGTCTGAATCCGctgcactgc < 1:1103210/35‑1 (MQ=255) tgtgGTAGCAACCGCGTCTGAATCCGCTGCACTTCAATACCTGGCACCGTATGCCGGTTGCGCAATGGGc > 1:1422916/1‑70 (MQ=255) tgtgGTAGCAACCGCGTCTGAATCCGCTGCACTTCAATACCTGGCACCGTATGCCGGTTGCGCAATGGGc > 1:3725458/1‑70 (MQ=255) tgtgGTAGCAACCGCGTCTGAATCCGCTGCACTTCAATACCTGGCACCGTATGCCGGTTGCGCAATGGGc > 1:4185923/1‑70 (MQ=255) gtgGTAGCAACCGCGTCTGAATCCGCTGCACTTCAATACCTGGCACCGTATGCCGGTTGCGCAATGGGCg < 1:911092/70‑1 (MQ=255) gtgGTAGCAACCGCGTCTGAATCCGCTGCACTTCAATACCTGGCACCGTATGCCGGTTGCGCAATGGGCg > 1:3608909/1‑70 (MQ=255) gtgGTAGCAACCGCGTCTGAATCCGCTGCACTTCAATACCTGGCACCGTATGCCGGTTGCGCAATGGGCg < 1:3880238/70‑1 (MQ=255) gtgGTAGCAACCGCGTCTGAATCCGCTGCACTTCAATACCTGGCACCGTATGCCGGTTGCGCAATGGGCg < 1:2679771/70‑1 (MQ=255) gtgGTAGCAACCGCGTCTGAATCCGCTGCACTTCAATACCTGGCACCGTATGCCGGTTGCGCAATGGGCg > 1:1188285/1‑70 (MQ=255) gtgGTAGCAACCGCGTCTGAATCCGCTGCACTTCAATACCTGGCACCGTATGCCGGTTGCGCAATGGGCg < 1:1971633/70‑1 (MQ=255) gtgGTAGCAACCGCGTCTGAATCCGCTGCACTTCAATACCTGGCACCGTATGCCGGTTGCGCAATGGGCg > 1:226786/1‑70 (MQ=255) gtgGTAGCAACCGCGTCTGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTGCGCAATGGGCg < 1:3946444/70‑1 (MQ=255) gtgGTAGCAACCGCGTCTGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTGCGCAATGGGCg < 1:1555517/70‑1 (MQ=255) gtgGTAGCAACCGCGTCTGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTGCGCAATGGGCg < 1:2289288/70‑1 (MQ=255) tgGTAGCAACCGCGTCTGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGaa < 1:4145336/71‑1 (MQ=255) gCAACCGCGTCTGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACtt < 1:1896420/71‑1 (MQ=255) cgcgTCTGAATCCGCTGCACTGCTATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTg < 1:1146822/71‑1 (MQ=255) gcgTTTGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGa > 1:2257971/1‑71 (MQ=255) gcgTCTGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGa > 1:1586812/1‑71 (MQ=255) gcgTCTGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGa > 1:3432602/1‑71 (MQ=255) gcgTCTGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGa > 1:2355702/1‑71 (MQ=255) gcgTCTGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGa > 1:1430379/1‑71 (MQ=255) gcgTCTGAATCCGCTGCACTGCAATACATGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGa > 1:3038994/1‑71 (MQ=255) gcgTATGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTgcg > 1:1493939/1‑49 (MQ=255) tCTGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACcg < 1:1822876/71‑1 (MQ=255) cTGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGt < 1:1451654/65‑1 (MQ=255) cTGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGt < 1:1467246/65‑1 (MQ=255) cTGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGt < 1:1977171/65‑1 (MQ=255) cTGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGt < 1:3757514/65‑1 (MQ=255) cTGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGt < 1:3937916/65‑1 (MQ=255) cTGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACcgc > 1:281662/1‑71 (MQ=255) cTGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACcgc > 1:1370656/1‑71 (MQ=255) tGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACcgcg > 1:1549545/1‑71 (MQ=255) tGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACcgcg > 1:1577478/1‑71 (MQ=255) tGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACcgcg > 1:3620530/1‑71 (MQ=255) tGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACcgcg > 1:2188546/1‑71 (MQ=255) tGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACcgcg > 1:85034/1‑71 (MQ=255) gAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACcgcg > 1:3098957/1‑70 (MQ=255) cGCTGCACTGCAATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACCGCGGTg > 1:530729/1‑68 (MQ=255) cGCTGCACTGCAATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACCGCGGTg > 1:2557654/1‑68 (MQ=255) cGCTGCACTGCAATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACCGCGGTg > 1:1915195/1‑68 (MQ=255) cGCTGCACTGCAATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACCGCGGTg > 1:3054082/1‑68 (MQ=255) cGCTGCACTGCAATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACCGCGGTg > 1:2978178/1‑68 (MQ=255) cGCTGCACTGCAATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACCGCGGTg > 1:2550827/1‑68 (MQ=255) gCTGCACTGCAATACCTGGCACCGTATGCCGGTTTCGCAATGGGCGAATACTTCCGTGACCGCGGTGAAGa < 1:4107455/71‑1 (MQ=255) gCTGCACTGCAATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACCGCGGTGAAGa < 1:1811087/71‑1 (MQ=255) ctgcacttcaATACCTGGCACCGTATGCCGGTTGCGCa < 1:310693/38‑1 (MQ=255) cactgcaATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACCGCgg > 1:3853650/1‑61 (MQ=255) cactgcaATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACCGCgg > 1:3505785/1‑61 (MQ=255) cactgcaATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACCGCgg > 1:2368552/1‑61 (MQ=255) cactgcaATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACCGCgg > 1:2435545/1‑61 (MQ=255) gcaATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACCGCGGTGAAGa > 1:3819661/1‑63 (MQ=255) gcaATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACCGCGGTGAAGa > 1:1996713/1‑63 (MQ=255) | ACTGGAAGAGCACGGCGCACTGGCTAACACCATCGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACCGCGGTGAAGA > minE/2467169‑2467300 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |