| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,489,241 | T→C | 10.7% | R80R (CGT→CGC) | dnaN → | DNA polymerase III, beta subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,489,241 | 0 | T | C | 10.7% | 143.9 / 10.2 | 56 | R80R (CGT→CGC) | dnaN | DNA polymerase III, beta subunit |
| Reads supporting (aligned to +/- strand): ref base T (26/24); new base C (2/4); total (28/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.69e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.11e-01 | |||||||||||
GCGCTGGTTCAGCCACACGAGCCAGGAGCGACGACCGTTCCGGCGCGCAAATTCTTTGATATCTGCCGTGGTCTGCCTGAAGGCGCGGAAATTGCCGTGCAGCTGGAAGGTGAACGGATGCTGGTACGCTCCGGGCG > minE/2489173‑2489309 | gcgcTGGTTCAGCCACACGAGCCAGGAGCGACGACCGTTCCGGCGCGCAAATTCTTTGATATCTGCCGTgg > 1:4159789/1‑71 (MQ=255) cTGGTTCAGCCACACGAGCCAGGAGCGACGACCGTTCCGGCGCGCAAATTCTTTGATATCTGCCGTGGTc < 1:43094/70‑1 (MQ=255) tCAGCCACACGAGCCAGGAGCGACGACCGTTCCGGCGCGCAAATTCTTTGATATCTGCCGTGGTCTGcc > 1:3715827/1‑69 (MQ=255) cAGCCACACGAGCCAGGAGCGACGACCGTTCCGGCGCGCAAATTCTTTGATATCTGCCGTGGTCTGCCTg < 1:779182/70‑1 (MQ=255) cAGCCACACGAGCCAGGAGCGACGACCGTTCCGGCGCGCAAATTCTTTGATATCTGCCGTGGTCTGCCTg < 1:646194/70‑1 (MQ=255) cAGCCACACGAGCCAGGAGCGACGACCGTTCCGGCGCGCAAATTCTTTGATATCTGCCGTGGTCTGCCTg < 1:1347543/70‑1 (MQ=255) aGCCACACGAGCCAGGAGCGACGACCGTTCCGGCGCGCAAATTCTTTGATATCTGCCGTGGTCTGCCTg < 1:2248596/69‑1 (MQ=255) gCCACACGAGCCAGGAGCGACGACCGTTCCGGCGCGCAAATTCTTTGATATCTGCCGTGGTCTGCCTGAAg > 1:151926/1‑71 (MQ=255) gCCACACGAGCCAGGAGCGACGACCGTTCCGGCGCGCAAATTCTTTGATATCTGCCGTGGTCTGCCTGAAg > 1:418294/1‑71 (MQ=255) cGAGCCAGGAGCGACGACCGTTCCGGCGCGCAAATTCTTTGATATCTGCCGTGGTCTGCCTGAAGGcgcg > 1:1891825/1‑70 (MQ=255) gAGCCAGGAGCGACGACCGTTCCGGCGCGCAAATTCTTTGATATCTGCCGTGGTCTGCCTGAAGGCGCGGa < 1:2490903/71‑1 (MQ=255) ccAGGAGCGACGACCGTTCCGGCGCGCAAATTCTTTGATATCTGCCGTGGTCTGCCTGAAGGCGCGGAAAt > 1:2366033/1‑71 (MQ=255) cAGGAGCGACGACCGTTCCGGCGCGCAAATTCTTTGATATCTGCCGTGGTCTGCCTGAAGGCGCGGAAAtt > 1:1426775/1‑71 (MQ=255) cAGGAGCGACGACCGTTCCGGCGCGCAAATTCTTTGATATCTGCCGTGGTCTGCCTGAAGGCGCGGAAAtt > 1:303584/1‑71 (MQ=255) gAGCGACGACCGTTGCGGCGCGCAAATTCTTTGATATCTGCCGTGGTCTGCCTGa < 1:648713/55‑1 (MQ=255) gAGCGACGACCGTTCCGGCGCGCAAATTCTTTGATATCTGCCGTGGTCTGCCTGa < 1:3667946/55‑1 (MQ=255) gAGCGACGACCGTTCCGGCGCGCAAATTCTTTGATATCTGCCGTGGTCTGCCTGa < 1:319162/55‑1 (MQ=255) gAGCGACGACCGTTCCGGCGCGCAAATTCTTTGATATCTGCCGTGGTCTGACTg < 1:3009964/54‑1 (MQ=255) aGCGACGACCGTTCCGGCGCGCAAATTCTTTGATATCTGCCGTGGTCTGCCTGAAGGCGCGGAAATTGCCg > 1:3724855/1‑71 (MQ=255) aGCGACGACCGTTCCGGCGCGCAAATTCTTTGATATCTGCCGTGGTCTGCCTGAAGGCGCGGAAATTGCCg > 1:1252572/1‑71 (MQ=255) aGCGACGACCGTTCCGGCGCGCAAATTCTTTGATATCTGCCGTGGTCTGCCTGAAGGCGCGGAAATTGCCg > 1:3907471/1‑71 (MQ=255) aGCGACGACCGTTCCGGCGCGCAAATTCTTTGATATCTGCCGTGGTCTGCCTGAAGGCGCGGAAATTGCCg > 1:3926920/1‑71 (MQ=255) gCGACGACCGTTCCGGCGCGCAAATTCTTTGATATCTGCCGTGGTCTGCCTGAAg > 1:2060697/1‑55 (MQ=255) gacgacCGTTCCGGCGCGCAAATTCTTTGATATCTGCCGTGGTCTGCCTGAAGGCGCGGAAATTGCCGTGc < 1:231390/71‑1 (MQ=255) ttCCGGCGCGCAAATTCTTTGATATCTGCCGTGGTCTGCCTg < 1:1980147/42‑1 (MQ=255) ttCCGGCGCGCAAATTCTTTGATATCTGCCGTGGTCTGCCTg < 1:1886695/42‑1 (MQ=255) ccGGCGCGCAAATTCTTTGATATCTGCCGTGGTCTGCCTGAAGGCGCGGa < 1:1197965/50‑1 (MQ=255) ccGGCGCGCAAATTCTTTGATATCTGCCGTGGTCTGCCTGAAGGCGCGGAAATTGCCGTGCAGCTGGAAgg > 1:844761/1‑71 (MQ=255) ccGGCGCGCAAATTCTTTGATATCTGCCGTGGTCTGCCTGAAGGCGCGGAAATTGCCGTGCAGCTGGAAg > 1:1587671/1‑70 (MQ=255) ggCGCGCAAATTCTTTGATATCTGCCGTGGTCTGCCTGAAGGCGCGGAAATTGCCGTGCAGCTGGAAGGTg < 1:2392815/71‑1 (MQ=255) gcAAATTCTTTGATATCTGCCGTGGTCTGCCTGAAGGCGCGGAAATTGCCGTGCAGCTGGAAGGTGAACg < 1:3906644/70‑1 (MQ=255) gcAAATTCTTTGATATCTGCCGTGGTCTGCCTGAAGGCGCGGAAATTGCCGTGCAGCTGGAAGGTGAACg > 1:3880914/1‑70 (MQ=255) aaaTTCTTTGATATCTGCCGTGGTCTGCCTGAAGGCGCGGAAATTGCCGTGCAGCTGGAAGGTGAACGGAt < 1:620191/71‑1 (MQ=255) aaaTTCTTTGATATCTGCCGTGGTCTGCCTGAAGGCGCGGAAATTGCCGTGCAGCTGGAAGGTGAACGGAt < 1:3211840/71‑1 (MQ=255) ttCTTTGATATTTGCCGTGGTCTGCCTGAAGGCGCGGAAATTGCCGTGCAGCTGGAAGGTGAACGGATGCt > 1:119195/1‑71 (MQ=255) ttCTTTGATATCTGCCGTGGTCTGCCTGAAGGCGCGGAAATTGCCGTGCAGCTGGAAGGTGaa > 1:1761695/1‑63 (MQ=255) tCTTTGATATCTGCCGTGGTCTGCCTGAAGGCGCGGAAATTGCCGTGCAGCTGGAAGGTGAACGGa > 1:988331/1‑66 (MQ=255) tttGATATCTGCCGTGGTCTGCCTGAAGGCGCGGAAATTGCCGTGCAGCTGGAAGGTGAACGGATGCTgg > 1:3309828/1‑70 (MQ=255) tttGATATCTGCCGTGGTCTGCCTGAAGGCGCGGAAATTGCCGTGCAGCTGGAAGGTGAACGGATGCTgg > 1:3938104/1‑70 (MQ=255) tGATATCTGCCGTGGTCTGCCTGAAGGCGCGGAAATTGCCGTGCAGCTGGAAGGTGAACGGATGCTgg < 1:2733676/68‑1 (MQ=255) tGATATCTGCCGCGGTCTGCCTGAAGGCGCGGAAATTGCCGTGCAGCTGGAAGGTGAACGGATGCTGGt < 1:385185/69‑1 (MQ=255) tGATATCTGCCGCGGTCTGCCTGAAGGCGCGGAAATTGCCGTGCAGCTGGAAGGTGAACGGATGCTGGt < 1:3295806/69‑1 (MQ=255) tGATATCTGCCGCGGTCTGCCTGAAGGCGCGGAAATTGCCGTGCAGCTGGAAGGTGAACGGATGCTGGt < 1:2015781/69‑1 (MQ=255) tGATATCTGCCGCGGTCTGCCTGAAGGCGCGGAAATTGCCGTGCAGCTGGAAGGTGAACGGATGCTGGt < 1:1456042/69‑1 (MQ=255) atatCTGCCGTGGTCTGCCTGAAGGCGCGGAAATTGCCGTGCAGCTGGAAGGTGAACgg < 1:862757/59‑1 (MQ=255) tGCCGTGGTCTGCCTGAAGGCGCGGAAATTGCCGTGCAGCTGGAAGGTGAACGGATGCTGGTACGCTCCgg > 1:687061/1‑71 (MQ=255) tGCCGTGGTCTGCCTGAAGGCGCGGAAATTGCCGTGCAGCTGGAAGGTGAACGGATGCTGGTACGCTCCgg > 1:3918990/1‑71 (MQ=255) tGCCGTGGTCTGCCTGAAGGCGCGGAAATTGCCGTGCAGCTGGAAGGTGAACGGATGCTGGTACGCTCCgg > 1:2530513/1‑71 (MQ=255) tGCCGTGGTCTGCCTGAAGGCGCGGAAATTGCCGTGCAGCTGGAAGGTGAACGGATGCTGGTACGCTCCgg > 1:2073631/1‑71 (MQ=255) tGCCGTGGTCTGCCTGAAGGCGCGGAAATTGCCGTGCAGCTGGAAGGTGAACGGATGCTGGTACGCTCCgg > 1:1580315/1‑71 (MQ=255) tGCCGCGGTCTGCCTGAAGGCGCGGAAATTGCCGTGCAGCTGGAAgg > 1:1032726/1‑47 (MQ=255) tGCCGCGGTCTGCCTGAAGGCGCGGAAATTGCCGTGCAGCTGGAAgg > 1:1027654/1‑47 (MQ=255) gCCGTGGTCTGCCTGAAGGCGCGGAAATTGCCGTGCAGCTGGa < 1:3833435/43‑1 (MQ=255) ccGTGGTCTGCCTGAAGGCGCGGAAATTGCCGTGCAGCTGGAAGGTGAACGGATGCTGGTACGCTCCGGGc < 1:3774532/71‑1 (MQ=255) cGTGGTCTGCCTGAAGGCGCGGAAATTGCCGTGCAGCTGGAAGGTGAACGGATGCTGGTACGCTCCGGGCg < 1:3091598/71‑1 (MQ=255) cGTGGTCTGCCTGAAGGCGCGGAAATTGCCGTGCAGCTGGAAGGTGAACGGATGCTGGTACGCTCCGGGCg < 1:1474917/71‑1 (MQ=255) | GCGCTGGTTCAGCCACACGAGCCAGGAGCGACGACCGTTCCGGCGCGCAAATTCTTTGATATCTGCCGTGGTCTGCCTGAAGGCGCGGAAATTGCCGTGCAGCTGGAAGGTGAACGGATGCTGGTACGCTCCGGGCG > minE/2489173‑2489309 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |