| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,523,551 | T→A | 5.6% | H55L (CAC→CTC) | dinD ← | DNA‑damage‑inducible protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,523,551 | 0 | T | A | 5.6% | 359.2 / 10.7 | 125 | H55L (CAC→CTC) | dinD | DNA‑damage‑inducible protein |
| Reads supporting (aligned to +/- strand): ref base T (51/67); new base A (5/2); total (56/69) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.41e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01 | |||||||||||
Only 100 of 140 total aligned reads displayed.
ATGCTCTTTTGGCATTTGAGCCAATTTTGACCATATCGAGGATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAGGTATAAAGTGACGATACTCTGAATAACC > minE/2523483‑2523621 | aTGCTCTTTTGGCATTTGAGCCAATTTTGACCATATCGAGGATCTCCTCGAAATGGTCATCAATtgtgtg < 1:121678/70‑1 (MQ=255) tGCTCTTTTGGCATTTGAGCCAATTTTGACCATATCGAGGATCTCCTCGAAATGGTCATCAATtgtgtg < 1:3270182/69‑1 (MQ=255) tGCTCTTTTGGCATTTGAGCCAATTTTGACCATATCGAGGATCTCCTCGAAATGGTCATCAATtgtgtg < 1:3941791/69‑1 (MQ=255) ctctTTTGGCATTTGAGCCAATTTTGACCATATCGAGGATCTCCTCGAAATGGTCATCAATTGTGTGACCa < 1:1372787/71‑1 (MQ=255) ctctTTTGGCATTTGAGCCAATTTTGACCATATCGAGGATCTCCTCGAAATGGTCATCAATTGTGTGACCa < 1:3760894/71‑1 (MQ=255) tctTTTGGCATTTGAGCCAATTTTGACCATATCGAGGATCTCCTCGAAATGGTCATCAATTGTGTGACCAc > 1:2175737/1‑71 (MQ=255) tctTTTGGCATTTGAGCCAATTTTGACCATATCGAGGATCTCCTCGAAATGGTCATCAATTGTGTGACCAc > 1:1627583/1‑71 (MQ=255) ctTTTGGCATTTGAGCCAATTTTGACCATATCGAGGATCTCCTCGAAATGGTCATCAATTGTGTGACCACt > 1:2485837/1‑71 (MQ=255) tttGGCATTTGAGACAATTTTGACCATATCGAGGATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGt > 1:4030250/1‑71 (MQ=255) ttGGCATTTGAGCCAATTTTGACCATATCGAGGATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGtt > 1:148163/1‑71 (MQ=255) ttGGCATTTGAGCCAATTTTGACCATATCGAGGATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGtt > 1:2509807/1‑71 (MQ=255) gCATTTGAGCCAATTTTGACCATATCGAGGATCTCCTCGAAATGGTCATCAATTGTGTGACTACTGTtct < 1:2587336/70‑1 (MQ=255) gCATTTGAGCCAATTTTGACCATATCGAGGATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTtct < 1:1329977/70‑1 (MQ=255) gCATTTGAGCCAATTTTGACCATATCGAGGATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTtct < 1:1395121/70‑1 (MQ=255) gCATTTGAGCCAATTTTGACCATATCGAGGATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTtct < 1:2127272/70‑1 (MQ=255) cATTTGAGCCAATTTTGACCATATCGAGGATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTtct < 1:2966129/69‑1 (MQ=255) ttGAGCCAATTTTGACCATATCGAGGATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTtctc > 1:2270685/1‑67 (MQ=255) ttGAGCCAATTTTGACCATATCGAGGATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTTCTca > 1:163260/1‑68 (MQ=255) ttGAGCCAATTTTGACCATATCGAGGATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTTCTCACAg > 1:4233716/1‑71 (MQ=255) ttGAGCCAATTTTGACCATATCGAGGATATCCTCGAAATGGTCATCAATTGTGTGACCACTGtt > 1:1942751/1‑64 (MQ=255) ccAATTTTGACCATATCGAGGATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGcttc < 1:847126/71‑1 (MQ=255) ccAATTTTGACCATATCGAGGATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGcttc < 1:4021030/71‑1 (MQ=255) ccAATTTTGACCATATCGAGGATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGcttc < 1:4214359/71‑1 (MQ=255) tttGACCATATCGAGGATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGct > 1:3066302/1‑64 (MQ=255) ttGACCATATCGAGGATCTCCTCGAAATGGTCATCAATTGTGTGa > 1:515070/1‑45 (MQ=255) ttGACCATATCGAGGATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTTCTCCCAg > 1:3274290/1‑60 (MQ=255) ccATATCGAGGATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGCTTCTTTGgcgcgc < 1:3197965/71‑1 (MQ=255) atatCGAGGATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGt > 1:2915006/1‑71 (MQ=255) atatCGAGGATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGt > 1:3358136/1‑71 (MQ=255) tatCGAGGATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTtct < 1:1166583/48‑1 (MQ=255) tatCGAGGATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTTCTca < 1:1680192/50‑1 (MQ=255) tatCGAGGATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTTCTca < 1:1974258/50‑1 (MQ=255) tatCGAGGATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTTCTca < 1:2722946/50‑1 (MQ=255) tatCGAGGATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGtt < 1:2128866/71‑1 (MQ=255) tatCGAGGATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGtt < 1:871809/71‑1 (MQ=255) tatCGAGGATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGtt < 1:3665626/71‑1 (MQ=255) tatCGAGGATCTCCTCGAAATGGTCATCAATTGTGAGAACAGTg < 1:3663983/44‑1 (MQ=255) tatCGAGGATCTCCTCGAAATGGTCATCAATTGTGAGAACAGTg < 1:384921/44‑1 (MQ=255) atCGAGGATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGtt > 1:902989/1‑70 (MQ=255) tCGAGGATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTaa < 1:3805777/71‑1 (MQ=255) tCGAGGATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTaa > 1:3720820/1‑71 (MQ=255) tCGAGGATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTa < 1:3165929/70‑1 (MQ=255) tCGAGGATCTCCTCGAAATGGTCATCAATTGTGAGAACAGTggt > 1:3521858/1‑42 (MQ=25) tCGAGGATCTCCTCGAAATGGTCATCAATTGTGAGAACAGTggt > 1:2912782/1‑42 (MQ=25) tCGAGGATCTCCTCGAAATGGTCATCAATTGTGAGAACAGTgg > 1:1841657/1‑42 (MQ=37) gAGGATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAAt < 1:29999/70‑1 (MQ=255) aGGATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATAc < 1:1193181/71‑1 (MQ=255) aGGATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATAc < 1:550264/71‑1 (MQ=255) aGGATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATAc < 1:419819/71‑1 (MQ=255) ggATCTCCTCGAAATTGTCATCAATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATa < 1:2257815/69‑1 (MQ=255) ggATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATa < 1:2162150/69‑1 (MQ=255) gATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTaa < 1:3181341/66‑1 (MQ=255) gATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTaa < 1:12230/66‑1 (MQ=255) aTCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACa > 1:3113724/1‑69 (MQ=255) aTCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAgg > 1:2395387/1‑71 (MQ=255) aTCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAgg > 1:3558895/1‑71 (MQ=255) aTCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAg > 1:1021338/1‑70 (MQ=255) ctcctcGAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGcttctt > 1:2319931/1‑50 (MQ=255) ctcctcGAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTaa > 1:1295933/1‑63 (MQ=255) ctcctcGAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTaa > 1:253590/1‑63 (MQ=255) ctcGAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAGGtata > 1:3374354/1‑70 (MQ=255) tcGAAATTGTCATCAATTGTGTGACCACTGTTCTCACAGGcttctt < 1:1217846/46‑1 (MQ=255) tcGAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGcttctt < 1:660133/46‑1 (MQ=255) cGAAATGGTCATCAATTGTGTGACCACTGTTCTcaca > 1:26830/1‑37 (MQ=255) cGAAATGGTCATCAATTGTGTGACCACTGTTCTcaca > 1:4189684/1‑37 (MQ=255) gAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAGGTATAAAGt > 1:3987722/1‑71 (MQ=255) gAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAGGTATAAAGt < 1:1321428/71‑1 (MQ=255) gAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAGGTATAAAGt > 1:3941033/1‑71 (MQ=255) gAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAGGTATAAAGt > 1:2417207/1‑71 (MQ=255) gAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAGGTATAAAGt > 1:3798550/1‑71 (MQ=255) tGGTCATCAATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAGGTATAAAGTGAc < 1:2985134/70‑1 (MQ=255) tGGTCATCAATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAGGTATAAAGTGAc < 1:1933135/70‑1 (MQ=255) catcaATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAgg > 1:1745200/1‑55 (MQ=255) catcaATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAgg > 1:944369/1‑55 (MQ=255) catcaATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAgg > 1:2726451/1‑55 (MQ=255) atcaATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTaa > 1:2450713/1‑48 (MQ=255) atcaATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATAc < 1:2555963/51‑1 (MQ=255) atcaATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATAc < 1:1620359/51‑1 (MQ=255) atcaATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATAc < 1:4004280/51‑1 (MQ=255) atcaATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAGGTATAAAGt < 1:1841637/62‑1 (MQ=255) atcaATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAGGTATAAAGTGACGATa < 1:3803575/69‑1 (MQ=255) tcaATTGTGTGACCACTGTTCTCACAGGCTTCTTTg < 1:361276/36‑1 (MQ=255) tcaATTGTGTGACCACTGTTCTCACAGGCTTCTTTg < 1:1297285/36‑1 (MQ=255) tcaATTGTGTGACCACTGTTCTCACAGGCTTCTTTg < 1:2214192/36‑1 (MQ=255) tcaATTGTGTGACCACTGTTCTCACAGGCTTCTTTg < 1:2547762/36‑1 (MQ=255) tcaATTGTGTGACCACTGTTCTCACAGGCTTCTTTg < 1:1747065/36‑1 (MQ=255) tcaATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAGGtat < 1:455188/56‑1 (MQ=255) caATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAGGTATAAAGTGa > 1:3870066/1‑62 (MQ=255) caATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAGGTATAAAGTGACGATAct > 1:1642996/1‑69 (MQ=255) aaTTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAGGTATAAAGTGACGATa > 1:1710747/1‑66 (MQ=255) aaTTGTGTCACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAGGTATAAAGTGACGATa < 1:666143/66‑1 (MQ=255) aTTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAGGTATAAAGt < 1:1358470/58‑1 (MQ=255) tgtgtgACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAGGTATaaa > 1:753328/1‑54 (MQ=255) gtgtgACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTaa > 1:976541/1‑41 (MQ=255) gtgtgACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAGGTATAAAGTGACGATACTCTGa > 1:3957409/1‑68 (MQ=255) gtgtgACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAGGTATAAAGTGACGATACTCTGAAt > 1:860899/1‑70 (MQ=255) gtgtgACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAGGTATAAAGTGACGATACTCTGAAt > 1:3350386/1‑70 (MQ=255) gtgtgACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAGGTATAAAGTGACGATACTCTGAAt > 1:1400988/1‑70 (MQ=255) gtgtgACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAGGTATAAAGTGACGATACTCTGAAt > 1:3975420/1‑70 (MQ=255) tgACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAGGTATAAAGTGACGATACTCTGAATAAcc > 1:832968/1‑71 (MQ=255) | ATGCTCTTTTGGCATTTGAGCCAATTTTGACCATATCGAGGATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAGGTATAAAGTGACGATACTCTGAATAACC > minE/2523483‑2523621 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |