| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,523,557 | C→G | 5.1% | S53T (AGT→ACT) | dinD ← | DNA‑damage‑inducible protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,523,557 | 0 | C | G | 5.1% | 428.6 / 6.7 | 136 | S53T (AGT→ACT) | dinD | DNA‑damage‑inducible protein |
| Reads supporting (aligned to +/- strand): ref base C (58/71); new base G (5/2); total (63/73) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.49e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 139 total aligned reads displayed.
TCTTTTGGCATTTGAGCCAATTTTGACCATATCGAGGATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAGGTATAAAGTGACGATACTCTGAATAACCCAGTAG > minE/2523487‑2523627 | ctTTTGGCATTTGAGCCAATTTTGACCATATCGAGGATCTCCTCGAAATGGTCATCAATTGTGTGACCACt > 1:2485837/1‑71 (MQ=255) tttGGCATTTGAGACAATTTTGACCATATCGAGGATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGt > 1:4030250/1‑71 (MQ=255) gCATTTGAGCCAATTTTGACCATATCGAGGATCTCCTCGAAATGGTCATCAATTGTGTGACTACTGTtct < 1:2587336/70‑1 (MQ=255) gCATTTGAGCCAATTTTGACCATATCGAGGATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTtct < 1:2798215/70‑1 (MQ=255) gCATTTGAGCCAATTTTGACCATATCGAGGATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTtct < 1:1329977/70‑1 (MQ=255) gCATTTGAGCCAATTTTGACCATATCGAGGATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTtct < 1:2127272/70‑1 (MQ=255) cATTTGAGCCAATTTTGACCATATCGAGGATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTtct < 1:2966129/69‑1 (MQ=255) ttGAGCCAATTTTGACCATATCGAGGATCTCCTCGAAATGGTCATCAATTGTGTGACCACTTTTCTCACAg > 1:163541/1‑71 (MQ=255) ttGAGCCAATTTTGACCATATCGAGGATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTTCTca > 1:163260/1‑68 (MQ=255) ttGAGCCAATTTTGACCATATCGAGGATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTTCTca > 1:1555599/1‑68 (MQ=255) ttGAGCCAATTTTGACCATATCGAGGATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTTCTCACAg > 1:4233716/1‑71 (MQ=255) tGAGCCAATTTTGACCATATCGAGGATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTTCTCACAg > 1:3254531/1‑70 (MQ=255) ccAATTTTGACCATATCGAGGATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGcttc < 1:3990991/71‑1 (MQ=255) ccAATTTTGACCATATCGAGGATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGcttc < 1:4021030/71‑1 (MQ=255) ccAATTTTGACCATATCGAGGATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGcttc < 1:4214359/71‑1 (MQ=255) ccAATTTTGACCATATCGAGGATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGcttc < 1:847126/71‑1 (MQ=255) tttGACCATATCGAGGATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGct > 1:3066302/1‑64 (MQ=255) ttGACCATATCGAGGATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTTCTCCCAg > 1:3274290/1‑60 (MQ=255) atatCGAGGATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGt > 1:3358136/1‑71 (MQ=255) atatCGAGGATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGt < 1:3603310/71‑1 (MQ=255) atatCGAGGATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGt > 1:2915006/1‑71 (MQ=255) tatCGAGGATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTtct < 1:1166583/48‑1 (MQ=255) tatCGAGGATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTTCTca < 1:1680192/50‑1 (MQ=255) tatCGAGGATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTTCTca < 1:1974258/50‑1 (MQ=255) tatCGAGGATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTTCTca < 1:2722946/50‑1 (MQ=255) tatCGAGGATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGtt < 1:3665626/71‑1 (MQ=255) tatCGAGGATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGtt < 1:871809/71‑1 (MQ=255) tatCGAGGATCTCCTCGAAATGGTCATCAATTGTGAGAACAGTg < 1:3663983/44‑1 (MQ=255) tatCGAGGATCTCCTCGAAATGGTCATCAATTGTGAGAACAGTg < 1:384921/44‑1 (MQ=255) atCGAGGATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGtt > 1:2084363/1‑70 (MQ=255) atCGAGGATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGtt > 1:902989/1‑70 (MQ=255) tCGAGGATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTaa > 1:3720820/1‑71 (MQ=255) tCGAGGATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTaa < 1:3805777/71‑1 (MQ=255) tCGAGGATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTa < 1:3165929/70‑1 (MQ=255) tCGAGGATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTa < 1:3791605/70‑1 (MQ=255) tCGAGGATCTCCTCGAAATGGTCATCAATTGTGAGAACAGTggt > 1:2912782/1‑42 (MQ=25) tCGAGGATCTCCTCGAAATGGTCATCAATTGTGAGAACAGTggt > 1:2297072/1‑42 (MQ=25) gAGGATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATa > 1:1299488/1‑71 (MQ=255) aGGATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATAc < 1:550264/71‑1 (MQ=255) aGGATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATAc < 1:419819/71‑1 (MQ=255) aGGATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATAc < 1:1193181/71‑1 (MQ=255) ggATCTCCTCGAAATTGTCATCAATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATa < 1:2257815/69‑1 (MQ=255) ggATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATa < 1:2162150/69‑1 (MQ=255) gATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGtt < 1:1346808/64‑1 (MQ=255) gATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTaa < 1:3181341/66‑1 (MQ=255) gATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTaa < 1:12230/66‑1 (MQ=255) aTCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACa > 1:1780398/1‑69 (MQ=255) aTCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACa > 1:3113724/1‑69 (MQ=255) aTCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAgg > 1:3558895/1‑71 (MQ=255) aTCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAg > 1:1021338/1‑70 (MQ=255) ctcctcGAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGcttctt > 1:2319931/1‑50 (MQ=255) ctcctcGAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTaa > 1:329188/1‑63 (MQ=255) ctcctcGAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTaa > 1:253590/1‑63 (MQ=255) cctcGAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGcttct < 1:1680596/47‑1 (MQ=255) ctcGAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAGGtata > 1:3374354/1‑70 (MQ=255) tcGAAATTGTCATCAATTGTGTGACCACTGTTCTCACAGGcttctt < 1:1217846/46‑1 (MQ=255) tcGAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGcttctt < 1:1222873/46‑1 (MQ=255) tcGAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGcttctt < 1:3465829/46‑1 (MQ=255) cGAAATGGTCATCAATTGTGTGACCACTGTTCTcaca > 1:26830/1‑37 (MQ=255) cGAAATGGTCATCAATTGTGTGACCACTGTTCTcaca > 1:4189684/1‑37 (MQ=255) gAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAGGTATAAAGt < 1:1398288/71‑1 (MQ=255) gAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAGGTATAAAGt > 1:3941033/1‑71 (MQ=255) gAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAGGTATAAAGt > 1:3987722/1‑71 (MQ=255) gAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAGGTATAAAGt > 1:2417207/1‑71 (MQ=255) aaaTGGTCATCAATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAGGTATAAAGt < 1:1867601/70‑1 (MQ=255) aTGGTCATCAATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAGGTATAAAGTGAc < 1:2441943/71‑1 (MQ=255) tGGTCATCAATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAGGTATAAAGTGAc < 1:1933135/70‑1 (MQ=255) tGGTCATCAATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAGGTATAAAGTGAc < 1:2985134/70‑1 (MQ=255) tcatcaATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAGGTATAAAGTGACGATa < 1:792880/71‑1 (MQ=255) catcaATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAgg > 1:944369/1‑55 (MQ=255) atcaATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTaa > 1:2247523/1‑48 (MQ=255) atcaATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATAc < 1:1620359/51‑1 (MQ=255) atcaATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATAc < 1:2555963/51‑1 (MQ=255) atcaATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAGGTATAAAGt < 1:1841637/62‑1 (MQ=255) atcaATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAGGTATAAAGTGACGATa < 1:3803575/69‑1 (MQ=255) tcaATTGTGTGACCACTGTTCTCACAGGCTTCTTTg < 1:2214192/36‑1 (MQ=255) tcaATTGTGTGACCACTGTTCTCACAGGCTTCTTTg < 1:2504958/36‑1 (MQ=255) tcaATTGTGTGACCACTGTTCTCACAGGCTTCTTTg < 1:1297285/36‑1 (MQ=255) tcaATTGTGTGACCACTGTTCTCACAGGCTTCTTTg < 1:2547762/36‑1 (MQ=255) tcaATTGTGTGACCACTGTTCTCACAGGCTTCTTTg < 1:624801/36‑1 (MQ=255) tcaATTGTGTGACCACTGTTCTCACAGGCTTCTTTg < 1:1747065/36‑1 (MQ=255) tcaATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAGGtat < 1:455188/56‑1 (MQ=255) caATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAGGTATAAAGTGa > 1:2153156/1‑62 (MQ=255) aaTTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAGGTATAAAGTGACGATa > 1:1710747/1‑66 (MQ=255) aaTTGTGTCACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAGGTATAAAGTGACGATa < 1:666143/66‑1 (MQ=255) aTTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAGGTATAAAGt < 1:1358470/58‑1 (MQ=255) tgtgtgACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAGGTATaaa > 1:753328/1‑54 (MQ=255) gtgtgACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTaa > 1:976541/1‑41 (MQ=255) gtgtgACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAGGTATAAAGTGACGATACTCTGa > 1:3957409/1‑68 (MQ=255) gtgtgACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAGGTATAAAGTGACGATACTCTGAAt > 1:3975420/1‑70 (MQ=255) gtgtgACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAGGTATAAAGTGACGATACTCTGAAt > 1:1400988/1‑70 (MQ=255) gtgtgACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAGGTATAAAGTGACGATACTCTGAAt > 1:860899/1‑70 (MQ=255) tgtgACCACTGTCCTCACAGGCTTCTTTGGCGCGCGTTAATACAGGTATAAAGTGACGATACTCTGAATa < 1:303231/70‑1 (MQ=255) tgACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAGGTATAAAGTGACGATACTCTGAATAAcc > 1:832968/1‑71 (MQ=255) aCCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAgg > 1:4186259/1‑42 (MQ=255) cACTGTTCTCACAGTCTTCTTTGGCGCGCGTTAATACAGGTATAAAGt < 1:1600449/48‑1 (MQ=255) cACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAGGTATAAAGt < 1:2411612/48‑1 (MQ=255) cACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAGGTATAAAGt < 1:26091/48‑1 (MQ=255) aCTGTTCTCAGAGGCTTCTTTGGCGCGCGTTAATACAGGTATAAAGTGACGATa < 1:2459227/54‑1 (MQ=255) cTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAGGTATAAAGTGACGATACTCTGAATAACCCagtag > 1:3663455/1‑71 (MQ=255) | TCTTTTGGCATTTGAGCCAATTTTGACCATATCGAGGATCTCCTCGAAATGGTCATCAATTGTGTGACCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAGGTATAAAGTGACGATACTCTGAATAACCCAGTAG > minE/2523487‑2523627 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |