| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,523,766 | T→A | 8.0% | intergenic (‑52/+169) | dinD ← / ← yicC | DNA‑damage‑inducible protein/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,523,766 | 0 | T | A | 8.0% | 488.5 / 29.5 | 174 | intergenic (‑52/+169) | dinD/yicC | DNA‑damage‑inducible protein/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (75/85); new base A (7/7); total (82/92) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01 | |||||||||||
Only 100 of 186 total aligned reads displayed.
GTTGATGATGTTCGTTCATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGCAAAATCTGGAGCAG > minE/2523696‑2523835 | gTTGATGATGTTCGTTCATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTAtt < 1:1845082/71‑1 (MQ=255) atgatgTTCGTTCATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTtata < 1:858252/71‑1 (MQ=255) atgatgTTCGTTCATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTtata < 1:3765687/71‑1 (MQ=255) gttcgttcATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAg > 1:2212702/1‑71 (MQ=255) gttcgttcATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAg > 1:3224711/1‑71 (MQ=255) gttcgttcATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAg > 1:503476/1‑71 (MQ=255) ttcgttcATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAg < 1:3776611/70‑1 (MQ=255) tcgttcATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAg > 1:4168630/1‑69 (MQ=255) tcgttcATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGtt > 1:2540677/1‑71 (MQ=255) tcgttcATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGt > 1:1508958/1‑70 (MQ=255) cgttcATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGtt < 1:3095636/70‑1 (MQ=255) cgttcATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGtt > 1:405251/1‑70 (MQ=255) cgttcATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGtt < 1:2399090/70‑1 (MQ=255) ttcATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTtgtg < 1:8229/71‑1 (MQ=255) tcATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTta > 1:3818459/1‑58 (MQ=255) cATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAg > 1:2500802/1‑71 (MQ=255) aTTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAg > 1:229269/1‑70 (MQ=255) aTTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAg > 1:4212102/1‑70 (MQ=255) aTTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAg < 1:2817279/70‑1 (MQ=255) aTTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAg > 1:28278/1‑70 (MQ=255) cccACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTAtc < 1:2955975/70‑1 (MQ=255) cACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCa < 1:2018030/71‑1 (MQ=255) cACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCa < 1:292932/71‑1 (MQ=255) aCTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCAc < 1:2974388/71‑1 (MQ=255) aCTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCAc < 1:55375/71‑1 (MQ=255) tacATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTta < 1:259432/45‑1 (MQ=255) tCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTta < 1:1944431/47‑1 (MQ=255) tCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTta < 1:2369077/47‑1 (MQ=255) ccATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGt > 1:1451432/1‑70 (MQ=255) cATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTtata > 1:741275/1‑47 (MQ=255) cATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTtata > 1:3392534/1‑47 (MQ=255) cATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTtata > 1:3690615/1‑47 (MQ=255) cATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGa > 1:2173962/1‑58 (MQ=255) cATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTtc < 1:2839567/71‑1 (MQ=255) cATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTtc > 1:3620866/1‑71 (MQ=255) cATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTtc > 1:3284108/1‑71 (MQ=255) cATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTtc > 1:2084154/1‑71 (MQ=255) gtgATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTa > 1:3094420/1‑58 (MQ=255) gtgATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTa > 1:1977874/1‑58 (MQ=255) tgATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGa > 1:3708867/1‑54 (MQ=255) tgATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATATAAATACAGtt > 1:3240221/1‑50 (MQ=38) atatCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAgtg < 1:2707018/71‑1 (MQ=255) atatCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAgtg < 1:3772978/71‑1 (MQ=255) tatCAATTGCAAAGAAAGTAAATCTGTAACTGTATTtatat < 1:3051559/41‑1 (MQ=255) tatCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCAc < 1:2193279/60‑1 (MQ=255) atCAATTGCAAAGAAAGTAAATCTGTAACTGTATTtata > 1:2607136/1‑39 (MQ=255) atCAATTGCAAAGAAAGTAAATCTGTAACTGTATTtata > 1:3482394/1‑39 (MQ=255) atCAATTGCAAAGAAAGTAAATCTGTAACTGTATTtata > 1:603244/1‑39 (MQ=255) atCAATTGCAAAGAAAGTAAATCTGTAACTGTATTtata > 1:2481326/1‑39 (MQ=255) atCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGtgtg > 1:484768/1‑71 (MQ=255) tCAATTGCAAAGATAGTAAATCTGTAACTGTATATAAATACAGt < 1:767892/44‑1 (MQ=25) tCAATTGCAAAGAAAGTAAATCTGTAACTGTAttt < 1:1378211/35‑1 (MQ=255) tCAATTGCAAAGAAAGTAAATCTGTAACTGTAttt < 1:3790285/35‑1 (MQ=255) tCAATTGCAAAGAAAGTAAATCTGTAACTGTAttt < 1:3352465/35‑1 (MQ=255) tCAATTGCAAAGAAAGTAAATCTGTAACTGTAttt < 1:2581852/35‑1 (MQ=255) tCAATTGCAAAGAAAGTAAATCTGTAACTGTATATAAATACAGt < 1:685103/44‑1 (MQ=37) tCAATTGCAAAGAAAGTAAATCTGTAACTGTATATAAATACAGt < 1:812440/44‑1 (MQ=37) tCAATTGCAAAGAAAGTAAATCTGTAACTGTATATAAATACAGt < 1:2571826/44‑1 (MQ=37) cAATTGCAAAGAAAGTAAATCTGTAACTGTATTtatata > 1:1966043/1‑39 (MQ=255) aaTTGCAAAGAGAGTAAATCTGTAACTGTATTtatata > 1:4197799/1‑38 (MQ=255) aaTTGCAAAGAAAGTAAATCTGTAACTGTATATAAATACAGtta > 1:1119101/1‑43 (MQ=25) gCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTAtct > 1:358338/1‑49 (MQ=255) gCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGc > 1:965066/1‑70 (MQ=255) cAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTtgtg < 1:2335931/41‑1 (MQ=255) cAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTtgtg < 1:138213/41‑1 (MQ=255) cAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCaa < 1:690417/71‑1 (MQ=255) aaagaaagTAAATCTGTAACTGTATTTATATACAGTtgtg < 1:403399/40‑1 (MQ=255) aagaaagTAAATCTGTAACTGTATTTATATACAGTTGTGAg > 1:1807408/1‑41 (MQ=255) aagaaagTAAATCTGTAACTGTATTTATATACAGTTGTGAg > 1:3652688/1‑41 (MQ=255) aagaaagTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGtt < 1:2063792/52‑1 (MQ=255) aagaaagTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGtt < 1:3746169/52‑1 (MQ=255) aagaaagTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGtt < 1:108440/52‑1 (MQ=255) aagaaagTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGtt < 1:4048503/52‑1 (MQ=255) aagaaagTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCt < 1:3410123/63‑1 (MQ=255) aagaaagTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCt < 1:2755407/63‑1 (MQ=255) aagaaagTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCt < 1:558200/63‑1 (MQ=255) agaaagTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTtctc > 1:3428098/1‑54 (MQ=255) agaaagTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTtctc > 1:3734170/1‑54 (MQ=255) agaaagTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCaaaaa < 1:1611046/71‑1 (MQ=255) agaaagTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCaaaa > 1:1173618/1‑70 (MQ=255) aaagTAATTCTGTAACTGTATTTATATACAGTTGTGAGTATCTCAc < 1:120713/46‑1 (MQ=255) aaagTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCaaaaaaa < 1:1047684/71‑1 (MQ=255) aaagTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCGGGGCaaaaaca < 1:1690570/71‑3 (MQ=255) aagTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCaaaaaaa < 1:3027554/70‑1 (MQ=255) agTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACa < 1:3861682/71‑1 (MQ=255) aaaTCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCg > 1:2132987/1‑71 (MQ=255) tAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGCaaaa < 1:1154728/69‑1 (MQ=255) tAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGCaaaa < 1:623856/69‑1 (MQ=255) tAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGCaaaa < 1:3778896/69‑1 (MQ=255) tAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGCaaa < 1:1547636/68‑1 (MQ=255) tAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGCaaa < 1:682779/68‑1 (MQ=255) tAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGCaa < 1:2652260/67‑1 (MQ=255) tAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGCAAAATc > 1:4089035/1‑71 (MQ=255) aCTGTATTTATATACAGTTGTGAGTATCTCACGTtct < 1:1366289/37‑1 (MQ=255) aCTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGCAAAATCTg > 1:1912954/1‑71 (MQ=255) tATTTATATACAGTTGTGAGTATCTCACGTTCTCAgtg > 1:3188738/1‑38 (MQ=255) tATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGCAAAATCTGGAg > 1:3771952/1‑70 (MQ=255) tATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGCAAAATCTGGAg > 1:4109837/1‑70 (MQ=255) tttATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGCAAAATCTg > 1:3897246/1‑65 (MQ=255) tttATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGCAAAATCTGGAGCa < 1:2745800/70‑1 (MQ=255) | GTTGATGATGTTCGTTCATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGCAAAATCTGGAGCAG > minE/2523696‑2523835 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |