| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,523,769 | T→A | 9.4% | intergenic (‑55/+166) | dinD ← / ← yicC | DNA‑damage‑inducible protein/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,523,769 | 0 | T | A | 9.4% | 413.2 / 30.6 | 149 | intergenic (‑55/+166) | dinD/yicC | DNA‑damage‑inducible protein/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (60/75); new base A (7/7); total (67/82) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.81e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.61e-01 | |||||||||||
Only 100 of 175 total aligned reads displayed.
ATGATGTTCGTTCATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGCAAAATCTGGAGCAGGC > minE/2523700‑2523837 | atgatgTTCGTTCATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTtata < 1:858252/71‑1 (MQ=255) gttcgttcATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAg > 1:2212702/1‑71 (MQ=255) gttcgttcATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAg > 1:3224711/1‑71 (MQ=255) gttcgttcATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAg > 1:503476/1‑71 (MQ=255) tcgttcATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAg > 1:4168630/1‑69 (MQ=255) tcgttcATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGtt > 1:2540677/1‑71 (MQ=255) tcgttcATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGt > 1:1508958/1‑70 (MQ=255) tcgttcATTGCCCACTCCATGTGATATAAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGtt > 1:1369298/1‑71 (MQ=255) cgttcATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGtt < 1:2399090/70‑1 (MQ=255) ttgaTTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTtgtg < 1:2192063/68‑1 (MQ=255) ttcATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTtgtg < 1:8229/71‑1 (MQ=255) ttcATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTtgtg < 1:3847574/71‑1 (MQ=255) ttcATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTtgtg < 1:2172409/71‑1 (MQ=255) tcATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTtgtg < 1:2576380/70‑1 (MQ=255) tcATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTtgt > 1:3057481/1‑69 (MQ=255) tcATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTtgt > 1:1950223/1‑69 (MQ=255) cATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAg > 1:3532545/1‑71 (MQ=255) cATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAg > 1:2500802/1‑71 (MQ=255) aTTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAg > 1:4212102/1‑70 (MQ=255) aTTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAg > 1:3975578/1‑70 (MQ=255) aTTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAg > 1:229269/1‑70 (MQ=255) aTTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAg > 1:3032543/1‑70 (MQ=255) aTTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAg < 1:2817279/70‑1 (MQ=255) cccACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTAtc < 1:2955975/70‑1 (MQ=255) ccACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTAtctc < 1:2706428/71‑1 (MQ=255) cACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCa < 1:292932/71‑1 (MQ=255) aCTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCAc < 1:55375/71‑1 (MQ=255) ccATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGt > 1:1451432/1‑70 (MQ=255) cATGTGATATCAATTGCAAAGAATGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTtc > 1:3027000/1‑71 (MQ=255) cATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTtata > 1:3392534/1‑47 (MQ=255) cATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTtata > 1:3690615/1‑47 (MQ=255) cATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTtata > 1:254800/1‑47 (MQ=255) cATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTtc > 1:3284108/1‑71 (MQ=255) cATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTtc > 1:4058445/1‑71 (MQ=255) cATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTtc < 1:2839567/71‑1 (MQ=255) cATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTtc > 1:3620866/1‑71 (MQ=255) tgtgATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTtctc < 1:3419595/71‑1 (MQ=255) tgtgATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTtctc < 1:373955/71‑1 (MQ=255) gtgATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTa > 1:3094420/1‑58 (MQ=255) gtgATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTtct > 1:3762494/1‑69 (MQ=255) tgATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGa > 1:3708867/1‑54 (MQ=255) atatCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAgtg < 1:3773098/71‑1 (MQ=255) tatCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCAc < 1:2193279/60‑1 (MQ=255) atCAATTGCAAAGAAAGTAAATCTGTAACTGTATTtata > 1:2607136/1‑39 (MQ=255) atCAATTGCAAAGAAAGTAAATCTGTAACTGTATTtata > 1:2481326/1‑39 (MQ=255) atCAATTGCAAAGAAAGTAAATCTGTAACTGTATTtata > 1:3230038/1‑39 (MQ=255) atCAATTGCAAAGAAAGTAAATCTGTAACTGTATTtata > 1:603244/1‑39 (MQ=255) atCAATTGCAAAGAAAGTAAATCTGTAACTGTATTtata < 1:678913/39‑1 (MQ=255) atCAATTGCAAAGAAAGTAAATCTGTAACTGTATTtata < 1:683769/39‑1 (MQ=255) atCAATTGCAAAGAAAGTAAATCTGTAACTGTATTtat > 1:2063137/1‑38 (MQ=255) atCAATTGCAAAGAAAGTAAATCTGTAACTGTATTtat > 1:4039263/1‑38 (MQ=255) atCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGtgtg > 1:484768/1‑71 (MQ=255) tCAATTGCAAAGAAAGTAAATCTGTAACTGTATATAAATACAGt < 1:377227/44‑1 (MQ=37) tCAATTGCAAAGAAAGTAAATCTGTAACTGTATATAAATACAGt < 1:2968128/44‑1 (MQ=37) cAATTGCAAAGAAAGTAAATCTGTAACTGTATTtatata > 1:1966043/1‑39 (MQ=255) aaTTGCAAAGAAAGTAAATCTTTAACTGTATTTATATACAGTtgt < 1:2372558/45‑1 (MQ=255) aaTTGCAAAGAAAGTAAATCTGTAACTGTATTtatata > 1:814314/1‑38 (MQ=255) aaTTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTtgt < 1:2736127/45‑1 (MQ=255) aaTTGCAAAGAAAGTAAATCTGTAACTGTATATAAATACAGtta > 1:784940/1‑43 (MQ=25) aaTTGCAAAGAAAGTAAATCTGTAACTGTATATAAATACAGtta > 1:1119101/1‑43 (MQ=25) gCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTAtct > 1:358338/1‑49 (MQ=255) gCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGc > 1:965066/1‑70 (MQ=255) cAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTtgtg < 1:3355435/41‑1 (MQ=255) cAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCaa < 1:690417/71‑1 (MQ=255) aaagaaagTAAATCTGTAACTGTATTTATATACAGTTGTGAGTAtctc < 1:737642/48‑1 (MQ=255) aagaaagTAAATCTGTAACTGTATTTATATACAGTTGTGAg > 1:4096312/1‑41 (MQ=255) aagaaagTAAATCTGTAACTGTATTTATATACAGTTGTGAg > 1:3652688/1‑41 (MQ=255) aagaaagTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGtt < 1:4048503/52‑1 (MQ=255) aagaaagTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGtt < 1:108440/52‑1 (MQ=255) aagaaagTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGtt < 1:17099/52‑1 (MQ=255) aagaaagTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGtt < 1:2063792/52‑1 (MQ=255) aagaaagTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCt < 1:345087/63‑1 (MQ=255) aagaaagTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCt < 1:558200/63‑1 (MQ=255) aagaaagTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCt < 1:4152303/63‑1 (MQ=255) agaaagTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTtctc > 1:2010090/1‑54 (MQ=255) agaaagTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCaaaaa < 1:1611046/71‑1 (MQ=255) agaaagTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCaaaaa < 1:1279214/71‑1 (MQ=255) agaaagTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCaaaa > 1:1173618/1‑70 (MQ=255) aaagTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCaaaaaaa < 1:1971588/71‑1 (MQ=255) aaagTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCaaaaaaa < 1:746741/71‑1 (MQ=255) aaagTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCaaaaaaa < 1:1197440/71‑1 (MQ=255) aaagTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCaaaaaaa < 1:1047684/71‑1 (MQ=255) aaagTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCGGGGCaaaaaca < 1:1690570/71‑3 (MQ=255) aaaTCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCg > 1:2813525/1‑71 (MQ=255) aaTCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCg > 1:2749989/1‑70 (MQ=255) tCTGTAACTGTATTTATATACAGTTGTGAGTATCTCAc > 1:3871578/1‑38 (MQ=255) tAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGCaaaa < 1:1154728/69‑1 (MQ=255) tAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGCaaaa < 1:3778896/69‑1 (MQ=255) tAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGCaaa < 1:1547636/68‑1 (MQ=255) tAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGCaa < 1:2652260/67‑1 (MQ=255) aCTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGCAAAATCTg > 1:1912954/1‑71 (MQ=255) tATTTATATACAGTTGTGAGTATCTCACGTTCTCAgtg > 1:3188738/1‑38 (MQ=255) tATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGCAAAATCTGGAg > 1:3771952/1‑70 (MQ=255) tATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGCAAAATCTGGAg > 1:614983/1‑70 (MQ=255) tATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGCAAAATCTGGAGc < 1:3799943/71‑1 (MQ=255) tttATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGCAAAATCTGGAGCAg > 1:2088997/1‑71 (MQ=255) tatataCAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGCAAAATCTGGAGCAgg < 1:1843124/70‑1 (MQ=255) tatataCAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGCAAAATCTGGAGCAgg > 1:4187652/1‑70 (MQ=255) tatataCAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGCAAAATCTGGAGCAgg > 1:3098082/1‑70 (MQ=255) atataCAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGCAAAATCTGGAGCAGGc < 1:3240953/70‑1 (MQ=255) | ATGATGTTCGTTCATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGCAAAATCTGGAGCAGGC > minE/2523700‑2523837 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |