| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,528,498 | C→T | 24.6% | D83N (GAT→AAT) | dfp ← | fused 4'‑phosphopantothenoylcysteine decarboxylase and phosphopantothenoylcysteine synthetase, FMN‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,528,498 | 0 | C | T | 24.6% | 116.3 / 37.6 | 61 | D83N (GAT→AAT) | dfp | fused 4'‑phosphopantothenoylcysteine decarboxylase and phosphopantothenoylcysteine synthetase, FMN‑binding |
| Reads supporting (aligned to +/- strand): ref base C (26/20); new base T (5/10); total (31/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.46e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01 | |||||||||||
TTCGCCATTCCGGCAGCAACACGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGCTTCGGCTGCCGGGTCCAGCAGACTGTCGG > minE/2528434‑2528563 | ttCGCCATTCCGGCAGCAACACGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCa > 1:1958821/1‑71 (MQ=255)ttCGCCATTCCGGCAGCAACACGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCa > 1:1438951/1‑71 (MQ=255) cGCCATTCCGGCAGCAACACGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCAtt > 1:4240056/1‑71 (MQ=255) gCCATTCCGGCAGCAACACGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAg > 1:166959/1‑64 (MQ=255) gCCATTCCGGCAGCAACACGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCAtt < 1:3806394/70‑1 (MQ=255) cGGCAGCAACACGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATTAGCCCATTTACCCAGc > 1:1671108/1‑71 (MQ=255) gcagcaACACGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTc < 1:3252569/71‑1 (MQ=255) gcagcaACACGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTc < 1:3762045/71‑1 (MQ=255) agcaACACGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATTAGCCCATTTACCCAGCTCaa < 1:405589/71‑1 (MQ=255) agcaACACGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATTAGCCCATTTACCCAGCTCaa < 1:3350347/71‑1 (MQ=255) agcaACACGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCaa < 1:1121420/71‑1 (MQ=255) agcaACACGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCaa < 1:4249336/71‑1 (MQ=255) agcaACACGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCaa < 1:1869007/71‑1 (MQ=255) agcaACACGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCaa < 1:1716111/71‑1 (MQ=255) agcaACACGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCaa < 1:1755502/71‑1 (MQ=255) tCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATTAGCCCATTTa < 1:1111345/47‑1 (MQ=255) tCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATTAGCCCATTTa < 1:1490542/47‑1 (MQ=255) tCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAATATGGCCCATAGCg < 1:1780547/71‑1 (MQ=255) tCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAATATGGCCCATAGCg > 1:3436097/1‑71 (MQ=255) tCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAATATGGCCCATAGCg < 1:2817920/71‑1 (MQ=255) aaaTCTGCCGTGGCAGGGGCGAGAATCACTAAATTAGCCCATTTACCCAGCTCAATATGGCCCATAGc < 1:3919517/68‑1 (MQ=255) aaaTCTGCCGTGGCAGGGGCGAGAATCACTAAATTAGCCCATTTACCCAGCTCAATATGGCCCATAGc < 1:2463705/68‑1 (MQ=255) aaaTCTGCCGTGGCAGGGGCGAGAATCACTAAATTAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGc > 1:667881/1‑71 (MQ=255) aaaTCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGc > 1:2555667/1‑71 (MQ=255) ccGTGGCAGGGGCGAGAATCACTAAATTAGCCCATTTACCCAGc < 1:2735566/44‑1 (MQ=255) ccGTGGCAGGGGCGAGAATCACTAAATTAGCCCATTTACCCAGc < 1:1019683/44‑1 (MQ=255) ccGTGGCAGGGGCGAGAATCACTAAATTAGCCCATTTACCCAGc < 1:2082936/44‑1 (MQ=255) ccGTGGCAGGGGCGAGAATCACTAAATTAGCCCATTTACCCAGc < 1:1821563/44‑1 (MQ=255) ccGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGCTTCgg < 1:4234392/69‑1 (MQ=255) ccGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGCTTCGGCt > 1:3857636/1‑71 (MQ=255) gTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGCTTCGGCTGc > 1:2375494/1‑71 (MQ=255) ggCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGCTTCGGCTGCCg > 1:446440/1‑71 (MQ=255) ggCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGCTTCGGCTGCCg > 1:272661/1‑71 (MQ=255) ggCAGGGGAGAGAATCACTAAATCAGCCCATTTACCCAGCTCAATATgg > 1:2876178/1‑49 (MQ=255) ggggCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGCTTCGGCTGcc < 1:1184835/66‑1 (MQ=255) ggggCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGCTTCGGCTGcc < 1:2834456/66‑1 (MQ=255) gggCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGCTTCGGCTGCCGGGTcc < 1:2776113/71‑1 (MQ=255) gCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGCTTCGGCTGCCGGGTCcag > 1:1606252/1‑71 (MQ=255) gagaATCACTAAATTAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGCTTCGGCTGCCGGGTCcag > 1:3095709/1‑69 (MQ=255) gagaATCACTAAATCAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGCTTCGGCTGCCGGGTCcagca < 1:3207455/71‑1 (MQ=255) gagaATCACTAAATCAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGCTTCGGCTGCCGGGTCcagca < 1:1854257/71‑1 (MQ=255) agaATCACTAAATCAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGCTTCGGCTGCCGGGTCcagcag < 1:907257/71‑1 (MQ=255) agaATCACTAAATCAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGCTTCGGCTGCCGGGTCcagcag < 1:1870713/71‑1 (MQ=255) gaATCACTAAATCAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGCTTCGGCTGCCGGGTCCAGCAGa > 1:3630238/1‑71 (MQ=255) aaTCACTAAATTAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGCTTCGGCTGCCGGGTCCAGCAGAc > 1:2473724/1‑71 (MQ=255) tCACTAAATCAGCCCATTTACCCAGCTCAATATgg > 1:3552627/1‑35 (MQ=255) tCACTAAATCAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGCTTCGGCTGCCGGGTCCAGCAGACTg > 1:2466393/1‑71 (MQ=255) tCACTAAATCAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGCTTCGGCTGCCGGGTCCAGCAGACTg > 1:1381647/1‑71 (MQ=255) cACTAAATCAGCCCATTTACCCAGCTCAATATGGCCCAt > 1:2570526/1‑39 (MQ=255) aaaTTAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGCTTCGGCTGCCGGGTCCAGCAGACTGTCgg > 1:806655/1‑70 (MQ=255) aaaTCAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGCTTCgg > 1:1681866/1‑46 (MQ=255) aaaTCAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGCTTCGGc > 1:3269266/1‑47 (MQ=255) aaaTCAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGCTTCGGc > 1:1330258/1‑47 (MQ=255) aaaTCAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGCTTCGGc > 1:2844517/1‑47 (MQ=255) aaaTCAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGCTTCGGc > 1:378548/1‑47 (MQ=255) aaaTCAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGCTTCGGc > 1:1860883/1‑47 (MQ=255) aaaTCAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGCTTCGGCTGCCGGGTCCAGCAGACTGTCgg > 1:3019657/1‑70 (MQ=255) aaTCAGCCCATTTACCCAGCTCAATATGGCCCATAg > 1:2930132/1‑36 (MQ=255) aaTCAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGCTTCGGCTGcc < 1:1743278/50‑1 (MQ=255) aaTCAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGCTTCGGCTGcc < 1:675650/50‑1 (MQ=255) aaTCAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGCTTCGGCTGCCgg > 1:4058196/1‑52 (MQ=255) | TTCGCCATTCCGGCAGCAACACGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGCTTCGGCTGCCGGGTCCAGCAGACTGTCGG > minE/2528434‑2528563 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |