| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 298,537 | C→T | 45.5% | L400L (CTG→TTG) | mdlA → | fused predicted multidrug transporter subunits and ATP‑binding components of ABC superfamily |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 298,537 | 0 | C | T | 45.5% | 22.7 / 105.9 | 77 | L400L (CTG→TTG) | mdlA | fused predicted multidrug transporter subunits and ATP‑binding components of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base C (21/21); new base T (15/20); total (36/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.47e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.23e-01 | |||||||||||
TACCCTGTTGTCGCTCATTCAGCGTCATTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAGTTAGCCAGACGCCATTCCTTTTT > minE/298467‑298605 | tACCCTGTTGTCGCTCATTCAGCGTCATTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCctc > 1:2324663/1‑71 (MQ=255)tACCCTGTTGTCGCTCATTCAGCGTCATTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCctc > 1:2868097/1‑71 (MQ=255) aCCCTGTTGTCGCTCATTCAGCGTCATTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCctc > 1:2108543/1‑70 (MQ=255) gtCGCTCATTCAGCGTCATTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGACGAAGt > 1:3624360/1‑71 (MQ=255) gtCGCTCATTCAGCGTCATTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGACGAAGt > 1:1517550/1‑71 (MQ=255) gtCGCTCATTCAGCGTCATTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGACGAAGt > 1:636618/1‑71 (MQ=255) tCGCTCATTCAGCGTCATTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTTTGACGAAGtt < 1:3623949/71‑1 (MQ=255) tCGCTCATTCAGCGTCATTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTTTGACGAAGtt < 1:3408050/71‑1 (MQ=255) gCTCATTCAGCGTCATTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGACGAAGTTAc < 1:1176810/71‑1 (MQ=255) gCTCATTCAGCGTCATTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGACGAAGTTAc < 1:2717650/71‑1 (MQ=255) gCTCATTCAGCGTCATTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGACGAAGTTAc < 1:80751/71‑1 (MQ=255) cTCATTCAGCGTCATTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTTTGACGAAGTTAc > 1:819551/1‑70 (MQ=255) cATTCAGCGTCATTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTTTGACGAAGTTACAAc > 1:1944369/1‑71 (MQ=255) cATTCAGCGTCATTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTTTGACGAAGTTACAAc > 1:3452158/1‑71 (MQ=255) ttCAGCGTCATTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTTTGACGAAGTTACAACTc < 1:3249827/71‑1 (MQ=255) ttCAGCGTCATTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTTTGACGAAGTTACAACTc < 1:695947/71‑1 (MQ=255) ttCAGCGTCATTTCGACGTCAGCGAGGGGCATATTCGCTTTCATGATATTCCTTTGACGAAGTTACAACTc < 1:3741520/71‑1 (MQ=255) tCAGCGTCATTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTTTGACGAAGTTACAACTc > 1:1933893/1‑70 (MQ=255) tCATTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGACGAAGTTACAACTc < 1:857225/64‑1 (MQ=255) tttCGACGTCAGCTAGGGGGATATTCGCTTTCATGATATTCCTCTGACGAAGTTACAACTCGATAGCTgg > 1:478479/1‑70 (MQ=255) tttCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGACGAAGTTACAACTCGAAAGCTGGc > 1:4045314/1‑71 (MQ=255) tCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTTTGACGAAGTTACAACTCGATAGCTGGCg < 1:2424737/70‑1 (MQ=255) tCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGACGAAGTTACaa > 1:3610518/1‑56 (MQ=255) tCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGACGAAGTTACaa > 1:2659218/1‑56 (MQ=255) gTCAGCGAGGGGGATATTCGCTTTCATGATATTCctct < 1:887381/38‑1 (MQ=255) gTCAGCGAGGGGGATATTCGCTTTCATGATATTCctct < 1:859594/38‑1 (MQ=255) aGCGAGGGGGATATTCGCTTTCATGATATTCCTTTGACGAAGTTACaa > 1:1818167/1‑48 (MQ=255) aGCGAGGGGGATATTCGCTTTCATGATATTCCTTTGACGAAGTTACaa > 1:2491219/1‑48 (MQ=255) aGCGAGGGGGATATTCGCTTTCATGATATTCCTTTGACGAAGTTACaa > 1:3687044/1‑48 (MQ=255) aGCGAGGGGGATATTCGCTTTCATGATATTCCTTTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCt > 1:1338595/1‑71 (MQ=255) aGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCt > 1:4226938/1‑71 (MQ=255) gCGAGGGGGATATTCGCTTTCATGATATTCCTCTGACGAAGTTACAACTCGATAGc < 1:2502673/56‑1 (MQ=255) gCGAGGGGGATATTCGCTTTCATGATATTCCTCTGACGAAGTTACAACTCGATAGc < 1:2148825/56‑1 (MQ=255) gCGAGGGGGATATTCGCTTTCATGATATTCCTCTGACGAAGTTACAACTCGATAGc < 1:1523333/56‑1 (MQ=255) cGAGGGGGATATTCGCTTTCATGATATTCCTCTGACGAAGTTACAACTCGATAGc < 1:624574/55‑1 (MQ=255) gggggATATTCGCTTTCATGATATTCCTCTGACGAAGTTACAAc < 1:2215726/44‑1 (MQ=255) ggggATATTCGCTTTCATGATATTCCTCTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGt < 1:3829800/71‑1 (MQ=255) gggATATTCGCTTTCATGATATTCCTCTGACGAAGTTACa < 1:359254/40‑1 (MQ=255) gggATATTCGCTTTCATGATATTCCTCTGACGAAGTTACAACTc > 1:2901733/1‑44 (MQ=255) gggATATTCGCTTTCATGATATTCCTCTGACGAAGTTACAACTc > 1:554952/1‑44 (MQ=255) ggATATTCGCTTTCATGATATTCCTCTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAg < 1:148108/71‑1 (MQ=255) ggATATTCGCTTTCATGATATTCCTCTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAg < 1:1299568/71‑1 (MQ=255) gATATTCGCTTTCATGATATTCCTCTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAg < 1:695033/70‑1 (MQ=255) atatTCGCTTTCATGATATTCCTTTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAGtt < 1:1811960/71‑1 (MQ=255) tatTCGCTTTCATGATATTCCTTTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAGTTa < 1:1309917/71‑1 (MQ=255) tatTCGCTTTCATGATATTCCTTTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAGTTa < 1:2443287/71‑1 (MQ=255) tatTCGCTTTCATGATATTCCTCTGACGAAGTTACAAc < 1:1831392/38‑1 (MQ=255) tatTCGCTTTCATGATATTCCTCTGACGAAGTTACAAc < 1:666894/38‑1 (MQ=255) tatTCGCTTTCATGATATTCCTCTGACGAAGTTACAAc < 1:2690467/38‑1 (MQ=255) tatTCGCTTTCATGATATTCCTCTGACGAAGTTACAAc < 1:397957/38‑1 (MQ=255) tatTCGCTTTCATGATATTCCTCTGACGAAGTTACAAc < 1:3940520/38‑1 (MQ=255) ttCGCTTTCATGATATTCCTCTGACGAAGTTACAACTCGATAGCTGGCGTAgc > 1:141609/1‑53 (MQ=255) ttCGCTTTCATGATATTCCTCTGACGAAGTTACAACTCGATAGCTGGCGTAgc > 1:182529/1‑53 (MQ=255) cGCTTTCATGATATTCCTTTGACGAAGTTACAACTCGa > 1:2533914/1‑38 (MQ=255) cGCTTTCATGATATTCCTTTGACGAAGTTACAACTCGa > 1:376693/1‑38 (MQ=255) cGCTTTCATGATATTCCTCTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAg > 1:1132067/1‑64 (MQ=255) gCTTTCATGATATTCCTCTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAGTTAGCCa < 1:3462589/70‑1 (MQ=255) tttCATGATATTCCTCTGATGAAGTTACAACTCGAt > 1:4224403/1‑36 (MQ=255) tttCATGATATTCCTCTGACGAAGTTACAACTCGAt > 1:1755693/1‑36 (MQ=255) tttCATGATATTCCTCTGACGAAGTTACAACTCGAt > 1:3177592/1‑36 (MQ=255) tttCATGATATTCCTCTGACGAAGTTACAACTCGAt > 1:810007/1‑36 (MQ=255) tttCATGATATTCCTCTGACGAAGTTACAACTCGAt > 1:3547859/1‑36 (MQ=255) cATGATATTCCTTTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAGTTAGcc > 1:3381213/1‑64 (MQ=255) cATGATATTCCTTTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAGTTAGcc > 1:3376395/1‑64 (MQ=255) cATGATATTCCTCTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAGTTAGCCAGACGc < 1:3040721/70‑1 (MQ=255) aTGATATTCCTCTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAGTTAGCCAGACGCCa > 1:704990/1‑71 (MQ=255) aTGATATTCCTCTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAGTTAGCCAGACGCCa > 1:2896178/1‑71 (MQ=255) ttCCTTTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAGTTAGCCAGACGCCATTCCt < 1:1375103/70‑1 (MQ=255) ttCCTTTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAGTTAGCCAGACGCCATTCCt < 1:778507/70‑1 (MQ=255) ttCCTTTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAGTTAGCCAGACGCCATTCCt < 1:2055432/70‑1 (MQ=255) ttCCTTTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAGTTAGCCAGACGCCATTCCt < 1:3065374/70‑1 (MQ=255) ttCCTTTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAGTTAGCCAGACGCCATTCCt < 1:4192618/70‑1 (MQ=255) ttCCTTTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAGTTAGCCAGACGCCATTCCt < 1:1754896/70‑1 (MQ=255) ttCCTTTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAGTTAGCCAGACGCCATTCCt < 1:3607692/70‑1 (MQ=255) ttCCTTTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAGTTAGCCAGACGCCATTCCt < 1:2590816/70‑1 (MQ=255) tCCTCTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAGTTAGCCAGACGCCAt > 1:2676071/1‑65 (MQ=255) ccTTTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAGTTAGCCAGACGCCATTCCtttt > 1:2546655/1‑71 (MQ=255) ccTTTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAGTTAGCCAGACGCCATTCCtttt > 1:1644854/1‑71 (MQ=255) ccTTTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAGTTAGCCAGACGCCATTCCtttt > 1:3966739/1‑71 (MQ=255) ccTTTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAGTTAGCCAGACGCCATTCCttt < 1:4031277/70‑1 (MQ=255) ccTTTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAGTTAGCCAGACGCCATTCCttt < 1:2141396/70‑1 (MQ=255) ccTTTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAGTTAGCCAGACGCCATTCCttt < 1:2152734/70‑1 (MQ=255) ctctGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAGTTAGCCAGACGCCATTCCttttt < 1:3092244/71‑1 (MQ=255) ctctGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAGTTAGCCAGACGCCATTCCttttt < 1:2209090/71‑1 (MQ=255) ctctGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAGTTAGCCAGACGCCATTCCtttt < 1:1668010/70‑1 (MQ=255) ctctGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAGTTAGCCAGACGCCATTCCtttt < 1:2779718/70‑1 (MQ=255) ctctGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAGTTAGCCAGACGCCATTCCtttt < 1:217341/70‑1 (MQ=255) tctGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAGTTAGCCAGACGCCATTCCtttt < 1:828361/69‑1 (MQ=255) | TACCCTGTTGTCGCTCATTCAGCGTCATTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAGTTAGCCAGACGCCATTCCTTTTT > minE/298467‑298605 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |