| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,531,609 | A→G | 64.2% | I14I (ATT→ATC) | coaD ← | pantetheine‑phosphate adenylyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,531,609 | 0 | A | G | 64.2% | 70.1 / 68.0 | 67 | I14I (ATT→ATC) | coaD | pantetheine‑phosphate adenylyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (13/11); new base G (18/25); total (31/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.44e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.23e-01 | |||||||||||
TCGCCAGAATAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTCAATGCGTTTTCGGTGGCAGG > minE/2531545‑2531679 | tCGCCAGAATAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGa > 1:205679/1‑71 (MQ=255) cGCCAGAATAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGAt > 1:3831027/1‑71 (MQ=255) cAGAATAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGATCGa < 1:751677/71‑1 (MQ=255) cAGAATAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGATCGa < 1:3062264/71‑1 (MQ=255) aCGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGATCGAAAGTAcc < 1:501099/71‑1 (MQ=255) aCGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGATCGAAAGTAc < 1:3327675/70‑1 (MQ=255) gTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCg > 1:2033381/1‑71 (MQ=255) gATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGATCGAAAGTACCCgg < 1:2571571/70‑1 (MQ=255) aTCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGATCGa < 1:1565558/59‑1 (MQ=255) aTCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGATCGa < 1:532214/59‑1 (MQ=255) aTCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGATCGa < 1:2231608/59‑1 (MQ=255) tCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGATCGAAAGTACCCGGATa < 1:1768260/71‑1 (MQ=255) tCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGATCGAAAGTACCCGGATa < 1:1773287/71‑1 (MQ=255) cGAAGATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGATc > 1:1087639/1‑55 (MQ=255) cGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGATCGAAAGTACCCGGATa > 1:2202748/1‑70 (MQ=255) cGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGATCGAAAGTACCCGGATa > 1:3176528/1‑70 (MQ=255) cGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGATCGAAAGTACCCGGATa > 1:1865324/1‑70 (MQ=255) cGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGATCGAAAGTACCCGGATa > 1:1234865/1‑70 (MQ=255) cGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGATCGAAAGTACCCGGATa > 1:3866122/1‑70 (MQ=255) gAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGATCGAAAGTACCCGGATaa < 1:2514677/70‑1 (MQ=255) gAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGATCGAAAGTACCCGGATaa < 1:1179245/70‑1 (MQ=255) gAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGATCGAAAGTACCCGGATaa < 1:1740743/70‑1 (MQ=255) gAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATggg < 1:2236288/51‑1 (MQ=255) aaCATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGATCGAAAGTACCCGGATaa < 1:450704/69‑1 (MQ=255) aCATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGATCGAAAGTACCCGGATAAAt > 1:3062011/1‑70 (MQ=255) tCTGCGTGGCGCGCGTCACGATATCGATGTGACCATTGGTGATGGGATCGAAAGTa < 1:2188996/56‑1 (MQ=255) tCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGcc > 1:682700/1‑71 (MQ=255) tCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGcc > 1:3840741/1‑71 (MQ=255) tCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGcc > 1:3005436/1‑71 (MQ=255) tCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGcc > 1:2939177/1‑71 (MQ=255) tCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGcc > 1:371847/1‑71 (MQ=255) cTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGATCGAAAGTACCCGGATAAATCGccc > 1:1396730/1‑71 (MQ=255) tGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGATCGAAAGTACCCGGATAAATCGCCCg > 1:619109/1‑71 (MQ=255) atcGTGACGCGCGCCACGATATCGATATGACCATTGGTGATGGGATCGAAAGTACCCGGATAAATCGCCCg > 1:3579149/3‑71 (MQ=255) atcGTGACGCGCGCCACGATATCGATATGACCATTGGTGATGGGATCGAAAGTACCCGGATAAATCGCCCg > 1:1712729/3‑71 (MQ=255) gcgcgcGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGttttt > 1:2219614/1‑70 (MQ=255) gcgcgcGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTg > 1:1896799/1‑71 (MQ=255) cgcgTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCAt < 1:2091960/71‑1 (MQ=255) gcgTCACGATATCGATATGACCATTGGTGATGGGATCGAAAGTACCCGGATAAATCGCCCGtt < 1:3744273/63‑1 (MQ=255) gcgTCACGATATCGATATGACCATTGGTGATGGGATCGAAAGTACCCGGATAAATCGCCCGtt < 1:1694755/63‑1 (MQ=255) gcgACACGATATCGATATGACCATTGGTGATGGGATCGAAAGTACCCGGATAAATCGCCCGtt < 1:2004298/63‑1 (MQ=255) tCACGATATCGATATGACCATTGTTAATGGGATCGAAAGTACCCGGATAAATCGCCCGtttt < 1:3917272/62‑1 (MQ=255) tCACGATATCGATATGACCATTGGTGATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATaaca < 1:1560531/71‑1 (MQ=255) tCACGATATCGATATGACCATTGGTGATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATaac < 1:375803/70‑1 (MQ=255) tCACGATATCGATATGACCATTGGTGATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATaac < 1:2569731/70‑1 (MQ=255) tCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATaa < 1:938122/50‑1 (MQ=255) tCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGtttt < 1:2196123/62‑1 (MQ=255) aCGATATCGATATGACCATTGGTGATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATaacaac > 1:387747/1‑71 (MQ=255) aCGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATaacaac > 1:3585132/1‑71 (MQ=255) aCGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGGTTTTGCATaacaac > 1:2078606/1‑71 (MQ=255) atatcgatatGACCATTGGTAATTGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTc < 1:962699/71‑1 (MQ=255) atatcgatatGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTc < 1:3380911/71‑1 (MQ=255) atatcgatatGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTc < 1:1883986/71‑1 (MQ=255) tcgatatGACCATTGGTGATGGGATCGAAAGTACCCGGATAAATCGCCCGtt < 1:1392505/52‑1 (MQ=255) cgatatGACCATTGGTGATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCa > 1:4044222/1‑57 (MQ=255) gatatGACCATTGGTGATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTCaa > 1:1194817/1‑68 (MQ=255) gatatGACCATTGGTGATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTCaa > 1:1602960/1‑68 (MQ=255) gatatGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTCAATGc < 1:1666520/71‑1 (MQ=255) gatatGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTCAATGc > 1:490565/1‑71 (MQ=255) gatatGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTCAATGc > 1:1885637/1‑71 (MQ=255) tGACCATTGGTGATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCAt > 1:663493/1‑53 (MQ=255) tGACCATTGGTGATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCAt > 1:2593069/1‑53 (MQ=255) aCCATTGGTGATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTCAATGCGTTTTc < 1:1721973/71‑1 (MQ=255) aCCATTGGTGATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTCAATGCGTTTTc < 1:594714/71‑1 (MQ=255) aCCATTGGTGATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTCAATGCGTTTTc < 1:788481/71‑1 (MQ=255) aCCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTCAATGCGTTTTc < 1:1818370/71‑1 (MQ=255) gTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTTCATAACAACCTCAATGCGTTTTCGGTGGc < 1:2386384/70‑1 (MQ=255) aaTGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTCAATGCGTTTTCGGTGGCAgg > 1:2855933/1‑71 (MQ=255) | TCGCCAGAATAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTCAATGCGTTTTCGGTGGCAGG > minE/2531545‑2531679 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |