| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,540,373 | G→T | 35.8% | D300Y (GAT→TAT) | rfaJ → | UDP‑D‑glucose:(galactosyl)lipopolysaccharide glucosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,540,373 | 0 | G | T | 35.8% | 208.4 / 92.5 | 117 | D300Y (GAT→TAT) | rfaJ | UDP‑D‑glucose:(galactosyl)lipopolysaccharide glucosyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (42/33); new base T (19/23); total (61/56) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.35e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01 | |||||||||||
Only 100 of 124 total aligned reads displayed.
TATCCATCGGTAAAATACTATAAAATAGCTTTAGAAAATTCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTAGTGCAACATCATTATATCTCA > minE/2540307‑2540441 | aTCCATCGGTAAAATACTATAAAATAGCTTTAGAAAATTCCCCCTGGAAAGATGACTCTCCACGAGATGc < 1:1061897/70‑1 (MQ=255) aTCCATCGGTAAAATACTATAAAATAGCTTTAGAAAATTCCCCCTGGAAAGATGACTCTCCACGAGATGc < 1:975268/70‑1 (MQ=255) aTCCATCGGTAAAATACTATAAAATAGCTTTAGAAAATTCCCCCTGGAAAGATGACTCTCCACGAGATGc < 1:2485749/70‑1 (MQ=255) aTCCATCGGTAAAATACTATAAAATAGCTTTAGAAAATTCCCCCTGGAAAGATGACTCTCCACGAGATGc < 1:1132704/70‑1 (MQ=255) ggTAAAATACTATAAAATAGCTTTAGAAAATTCCCCCTGGAAAGATGACTCTCCACGATATGCGAAATCa > 1:3241231/1‑70 (MQ=255) ggTAAAATACTATAAAATAGCTTTAGAAAATTCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATCaa > 1:3182360/1‑71 (MQ=255) ggTAAAATACTATAAAATAGCTTTAGAAAATTCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATCaa > 1:645963/1‑71 (MQ=255) aaaaTACTATAAAATAGCTTTAGAAAATTCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATCAat < 1:3580103/69‑1 (MQ=255) aaaTACTATAAAATAGCTTTAGAAAATTCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATCAattat > 1:1401491/1‑71 (MQ=255) aaaTACTATAAAATAGCTTTAGAAAATTCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATCAattat > 1:2549313/1‑71 (MQ=255) aaaTACTATAAAATAGCTTTAGAAAATTCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATCAattat > 1:1685663/1‑71 (MQ=255) aaTACTATAAAATAGCTTTAGAAAATTCCCCCTGGAAAGATGACTCTCCACGATATGCGAAATCAat < 1:1073703/67‑1 (MQ=255) aTACTATAAAATAGCTTTAGAAAATTCCCCCTGGAAAGATGACTCTCCACGATATGCGAAATCAattatt > 1:738845/1‑70 (MQ=255) tACTATAAAATAGCTTTAGAAAATTCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATCAatta > 1:4251448/1‑67 (MQ=255) tACTATAAAATAGCTTTAGAAAATTCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATCAatta > 1:2756002/1‑67 (MQ=255) taAAATAGCTTTAGAAAATTCCCCCTGGAAAGATGACTCTCCACGATATGCGAAATCAATTATTGAATTTa < 1:3297665/71‑1 (MQ=255) aaaaTAGCTTTAGAAAATTCCCCCTGGAAAGATGACTCTCCACGATATGCGAAATCAATTATTGAATTTa > 1:1649901/1‑70 (MQ=255) aaaaTAGCTTTAGAAAATTCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATCAATTATTGAAtt > 1:3577000/1‑68 (MQ=255) aaaTAGCTTTAGAAAATTCCCCCTGGAAAGATGACTCTCCACGATATGCGaaa > 1:3781814/1‑53 (MQ=255) aaaTAGCTTTAGAAAATTCCCCCTGGAAAGATGACTCTCCACGATATGCGaaa > 1:1752946/1‑53 (MQ=255) aaaTAGCTTTAGAAAATTCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATCAATTATTGAATTTaa > 1:813879/1‑70 (MQ=255) ttAGAAAATTCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGat > 1:625771/1‑70 (MQ=255) tAGAAAATTCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGata > 1:2309475/1‑70 (MQ=255) tAGAAAATTCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGata > 1:2463454/1‑70 (MQ=255) tAGAAAATTCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGat < 1:820550/69‑1 (MQ=255) tAGAAAATTCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGat < 1:2340814/69‑1 (MQ=255) gAAAATTCCCCCTGGAAAGATGACTCTCCACGATATGCGAAATCAATTATTGAATTTAAAAAAAGAtata < 1:2146550/70‑1 (MQ=255) gAAAATTCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGAtata < 1:1706807/70‑1 (MQ=255) gAAAATTCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGAtata < 1:3550939/70‑1 (MQ=255) gAAAATTCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGAtata < 1:1358000/70‑1 (MQ=255) aaaaTTCCCCCTGGAAAGATGACTCTCCACGATATGCGAAATCAATTATTGAATTTAAAAAAAGAtata < 1:3329387/69‑1 (MQ=255) aaaaTTCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATCAattatt < 1:2537927/50‑1 (MQ=255) aaTTCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATc > 1:965326/1‑41 (MQ=255) aTTCCCCCTGGAAAGATGACTCTCCACGATATGCGAAATCAat < 1:347072/43‑1 (MQ=255) aTTCCCCCTGGAAAGATGACTCTCCACGATATGCGAAATCAat < 1:2139713/43‑1 (MQ=255) aTTCCCCCTGGAAAGATGACTCTCCACGATATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACa < 1:801769/70‑1 (MQ=255) ccccTGGAAAGATGACTCTCCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCttt < 1:3435241/71‑1 (MQ=255) ccccTGGAAAGATGACTCTCCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCttt < 1:3033007/71‑1 (MQ=255) ccccTGGAAAGATGACTCTCCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCtt < 1:339549/70‑1 (MQ=255) cccTGGAAAGATGACTCTCCACGATATGCGAAATCAATTATTGAATTTAAAAAAAGAtata < 1:500109/61‑1 (MQ=255) cccTGGAAAGATGACTCTCCACGATATGCGAAATCAATTATTGAATTTAAAAAAAGAtata < 1:685999/61‑1 (MQ=255) ccTGGAAAGATGACTCTCCACGATATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTa < 1:1486940/71‑1 (MQ=255) cTGGAAAGATGACTCTCCACGAGATGCGAAATCAat > 1:3217869/1‑36 (MQ=255) cTGGAAAGATGACTCTCCACGAGATGCGAAATCAat > 1:2642505/1‑36 (MQ=255) cTGGAAAGATGACTCTCCACGAGATGCGAAATCAat > 1:1767116/1‑36 (MQ=255) cTGGAAAGATGACTCTCCACGAGATGCGAAATCAat > 1:2023358/1‑36 (MQ=255) tGGAAAGATGACTCTCCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCtttt < 1:1912009/68‑1 (MQ=255) tGGAAAGATGACTCTCCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCtttt < 1:548321/68‑1 (MQ=255) tGGAAAGATGACTCTCCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTAGt > 1:2862189/1‑71 (MQ=255) ggAAAGATGACTCTCCACGATATGCGAAATCAATTATTGAATTTaaaa < 1:876441/48‑1 (MQ=255) ggAAAGATGACTCTCCACGATATGCGAAATCAATTATTGAATTTaaaa < 1:1062933/48‑1 (MQ=255) ggAAAGATGACTCTCCACGATATGCGAAATCAATTATTGAATTTAAAAAAAGAtata < 1:1888071/57‑1 (MQ=255) ggAAAGATGACTCTCCACGATATGCGAAATCAATTATTGAATTTAAAAAAAGAtata < 1:2174621/57‑1 (MQ=255) ggAAAGATGACTCTCCACGATATGCGAAATCAATTATTGAATTTAAAAAAAGAtata < 1:4215231/57‑1 (MQ=255) ggAAAGATGACTCTCCACGATATGCGAAATCAATTATTGAATTTAAAAAAAGAtata < 1:2268311/57‑1 (MQ=255) ggAAAGATGACTCTCCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTAGTg > 1:721953/1‑71 (MQ=255) ggAAAGATGACTCTCCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTAGTg > 1:2254235/1‑71 (MQ=255) ggAAAGATGACTCTCCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTAGTg > 1:4186541/1‑71 (MQ=255) gAAAGATGACTCTCCACGAGATGCGAAATCAATTATTGAATTTaaaaaa > 1:3691340/1‑49 (MQ=255) gAAAGATGACTCT‑CACGAGATGCGAAATCAATTATTGAATTTaaaaaaa > 1:143867/1‑49 (MQ=255) aaaGATGACTCTCCACGATATGCGAAATCAATTATTGAATTTaaaaaa > 1:1785319/1‑48 (MQ=255) gATGACTCTCCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTAGTGCAAca < 1:2453596/71‑1 (MQ=255) gATGACTCTCCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTAGTGCAAca < 1:3836300/71‑1 (MQ=255) tGACTCTCCAAGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTa < 1:3027533/61‑1 (MQ=255) aCTCTCCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTAGTGCAAcatcat < 1:3521369/71‑1 (MQ=255) ctctCCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTAGTGCAACATCAtt < 1:1163727/71‑1 (MQ=255) tctcCACGATATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCtttt < 1:3297451/56‑1 (MQ=255) tctcCACGATATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCtttt < 1:2970091/56‑1 (MQ=255) tctcCACGATATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCtttt < 1:4135015/56‑1 (MQ=255) tctcCACGATATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCtttt < 1:1806282/56‑1 (MQ=255) tctcCACGATATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTa > 1:4001206/1‑57 (MQ=255) ctcCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGAtata < 1:3715725/45‑1 (MQ=255) tcCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGatat < 1:1593438/43‑1 (MQ=255) tcCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGatat < 1:113311/43‑1 (MQ=255) tcCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACAt < 1:2892802/49‑1 (MQ=255) tcCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACAt < 1:2202580/49‑1 (MQ=255) tcCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTAGt > 1:3066720/1‑57 (MQ=255) ccACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATaaa > 1:559494/1‑45 (MQ=255) ccACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATaaa > 1:3917439/1‑45 (MQ=255) cACGATATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTAGTGCAAcatc > 1:398950/1‑63 (MQ=255) cACGATATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTAGTGCAAcatc > 1:1147504/1‑63 (MQ=255) cACGATATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTAGTGCAAc > 1:2729721/1‑60 (MQ=255) cACGATATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTAGTGCAAc > 1:1508148/1‑60 (MQ=255) cACGATATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTAGTGCAACATCATTATAtc > 1:1070217/1‑71 (MQ=255) cACGATATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTAGTGCAACATCATTATAtc > 1:2519501/1‑71 (MQ=255) cACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATaa > 1:3447830/1‑43 (MQ=255) cACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATaa > 1:2656842/1‑43 (MQ=255) cACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCtt > 1:1560470/1‑50 (MQ=255) cACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTAGTGCAACATCATtat < 1:479006/68‑1 (MQ=255) cACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTAGTGCAACATCATtat < 1:4041291/68‑1 (MQ=255) cGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTAGTGCAACATCATTATAtct > 1:1898831/1‑70 (MQ=255) cGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTAGTGCAACATCATTATAtct > 1:1833663/1‑70 (MQ=255) cGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTAGTGCAACATCATTATAtct > 1:1016761/1‑70 (MQ=255) gagaTGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTAGTGCAACATCATTATATCTCa < 1:3808719/71‑1 (MQ=255) gagaTGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTAGTGCAACATCATTATATCTCa < 1:1391220/71‑1 (MQ=255) agaTGCGAAATCTATTATTGTATTTAAAAAAAGATATAAACATCttt < 1:958054/47‑1 (MQ=255) agaTGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCttt < 1:423686/47‑1 (MQ=255) agaTGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCttt < 1:3162240/47‑1 (MQ=255) agaTGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCttt < 1:2683444/47‑1 (MQ=255) gaTGCGAAATCAATTATTGAATTTAAAAAAAGAtata < 1:1685755/37‑1 (MQ=255) | TATCCATCGGTAAAATACTATAAAATAGCTTTAGAAAATTCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTAGTGCAACATCATTATATCTCA > minE/2540307‑2540441 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |