| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,599,135 | A→T | 29.5% | I33I (ATT→ATA) | gor ← | glutathione oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,599,135 | 0 | A | T | 29.5% | 86.2 / 51.0 | 61 | I33I (ATT→ATA) | gor | glutathione oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base A (25/18); new base T (15/3); total (40/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.90e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.84e-01 | |||||||||||
CACATCACTTTTTTCGGCACACAGCCAACATTTACGCAGGTGCCGCCCAGCTCTTTGGCTTCAATCAGCGCACATTTCTGGCCGTACATAGCCGCGCGGTTGATGGAGGCGATACCGCCGCTGCCGCCGCCG > minE/2599073‑2599204 | cacaTCACTTTTTTCGGCACACAGCCAACATTTACGCAGGTGCCGCCCAGCTCTTTGGCTTCAATCAgcgc < 1:611674/71‑1 (MQ=255) aCTTTTTTCGGCACACAGCCAACATTTACGCAGGTGCCGCCCAGCTCTTTGGCTTCTATCAgc > 1:502609/1‑63 (MQ=255) aCTTTTTTCGGCACACAGCCAACATTTACGCAGGTGCCGCCCAGCTCTTTGGCTTCTATCAgc > 1:2503417/1‑63 (MQ=255) aCTTTTTTCGGCACACAGCCAACATTTACGCAGGTGCCGCCCAGCTCTTTGGCTTCTATCAGCGCACAttt < 1:1649027/71‑1 (MQ=255) aCTTTTTTCGGCACACAGCCAACATTTACGCAGGTGCCGCCCAGCTCTTTGGCTTCAATCAg > 1:2779727/1‑62 (MQ=255) aCTTTTTTCGGCACACAGCCAACATTTACGCAGGTGCCGCCCAGCTCTTTGGCTTCAATCAGCGCACAttt > 1:3801954/1‑71 (MQ=255) aCTTTTTTCGGCACACAGCCAACATTTACGCAGGTGCCGCCCAGCTCTTTGGCTTCAATCAGCGCACAttt > 1:2001269/1‑71 (MQ=255) aCTTTTTTCGGCACACAGCCAACATTTACGCAGGTGCCGCCCAGCTCTTTGGCTTCAATCAGCGCACAttt > 1:1473435/1‑71 (MQ=255) ttttCGGCACACAGCCAACATTTACGCAGGTGCCGCCCAGCTCTTTGGCTTCTATCAGCGCACATTTCTgg > 1:2176152/1‑71 (MQ=255) ttttCGGCACACAGCCAACATTTACGCAGGTGCCGCCCAGCTCTTTGGCTTCAATCAGCGCACAttt > 1:1880418/1‑67 (MQ=255) ttttCGGCACACAGCCAACATTTACGCAGGTGCCGCCCAGCTCTTTGGCTTCAATCAGCGCACATTTCTg > 1:1238572/1‑70 (MQ=255) ttttCGGCACACAGCCAACATTTACGCAGGTGCCGCCCAGCTCTTTGGCTTCAATCAGCGCACATTTCTg > 1:1692615/1‑70 (MQ=255) ttttCGGCACACAGCCAACATTTACGCAGGTGCCGCCCAGCTCTTTGGCTTCAATCAGCGCACATTTCTg > 1:315405/1‑70 (MQ=255) ttttCGGCACACAGCCAACATTTACGCAGGTGCCGCCCAGCTCTTTGGCTTCAATCAGCGCACATTTCTg > 1:2723358/1‑70 (MQ=255) ggCACACAGCCAACATTTACGCAGGTGCCGCCCAGCTCTTTGGCTTCTATCAGCGCACATTTCTGGCCGTa < 1:513276/71‑1 (MQ=255) caGCCAACATTTACGCAGGTGCCGCCCAGCTCTTTGGCTTCAATc > 1:776518/1‑45 (MQ=255) caGCCAACATTTACGCAGGTGCCGCCCAGCTCTTTGGCTTCAATc > 1:2625968/1‑45 (MQ=255) gCCAACATTTACGCAGGTGCCGCCCAGCTCTTTGGCTTCAATCa < 1:2098059/44‑1 (MQ=255) cAACATTTACGCAGGTGCCGCCCAGCTCTTTGGCTTCAATCAGTGca < 1:2445678/47‑1 (MQ=255) cAACATTTACGCAGGTGCCGCCCAGCTCTTTGGCTTCAATCAGCGca < 1:2017411/47‑1 (MQ=255) cAACATTTACGCAGGTGCCGCCCAGCTCTTTGGCTTCAATCAGCGca < 1:2032834/47‑1 (MQ=255) aaCATTTACGCAGGTGCCGCCCAGCTCTTTGGCTTCAATCAGCGCACAt < 1:930479/49‑1 (MQ=255) aaCATTTACGCAGGTGCCGCCCAGCTCTTTGGCTTCAATCAGCGCACAt < 1:2084175/49‑1 (MQ=255) cATTTACGCAGGTGCCGCCCAGCTCTTTGGCTTCAATCAGCGCACATTTCTGGCCGTACATAGCCGCGCgg > 1:1813482/1‑71 (MQ=255) cATTTACGCAGGTGCCGCCCAGCTCTTTGGCTTCAATCAGCGCACATTTCTGGCCGTACATAGCCGCGCgg > 1:3920496/1‑71 (MQ=255) tttACGCAGGTGCCGCCCAGCTCTTTGGCTTCAATCAGCGCACATTTCTGGCCGTACATAGCCGCGCGGt < 1:1209065/70‑1 (MQ=255) tACGCAGGTGCCGCCCAGCTCTTTGGCTTCAATCAGCGCACATTTCTGGCCGTACATa > 1:2384874/1‑58 (MQ=255) tACGCAGGTGCCGCCCAGCTCTTTGGCTTCAATCAGCGCACATTTCTGGCCGTACATa > 1:1418861/1‑58 (MQ=255) aCGCAGGTGCCGCCCAGCTCTTTGGCTTCTATCAGCGcac > 1:3864265/1‑40 (MQ=255) aCGCAGGTGCCGCCCAGCTCTTTGGCTTCTATCAGCGCACATTTCt > 1:535477/1‑46 (MQ=255) aCGCAGGTGCCGCCCAGCTCTTTGGCTTCTATCAGCGCACATTTCt > 1:2432582/1‑46 (MQ=255) aCGCAGGTGCCGCCCAGCTCTTTGGCTTCTATCAGCGCACATTTCt > 1:2415091/1‑46 (MQ=255) aCGCAGGTGCCGCCCAGCTCTTTGGCTTCAATCAGCGCACATTTCTGGCCGTACATAGCCGCGCGGTTGa < 1:1403488/70‑1 (MQ=255) aCGCAGGTGCCGCCCAGCTCTTTGGCTTCAATCAGCGCACATTTCTGGCCGTACATAGCCGCGCGGTTGa < 1:272070/70‑1 (MQ=255) aCGCAGGTGCCGCCCAGCTCTTTGGCTTCAATCAGCGCACATTTCTGGCCGTACATAGCCGCGCGGTTGa < 1:3692887/70‑1 (MQ=255) aCGCAGGTGC‑‑CCCAGCTCTTTGGCTTCTATCAGCGCACATTTCt > 1:1465357/1‑44 (MQ=255) cGCAGGTGCCGCCCAGCTCTTTGGCTTCAATCAGCGCACATTTCTGGCCGTACATAGCCGCGCGGTTGa < 1:193015/69‑1 (MQ=255) cGCAGGTGCCGCCCAGCTCTTTGGCTTCAATCAGCGCACATTTCTGGCCGTACATAGCCGCGCGGTTGATg < 1:4156975/71‑1 (MQ=255) gccgccCAGCTCTTTGGCTTCTATCAGCGCACATTTCTGGCCGTACATAGCCGCGCGGTTGATGGAGGCGa > 1:3222497/1‑71 (MQ=255) gccgccCAGCTCTTTGGCTTCTATCAGCGCACATTTCTGGCCGTACATAGCCGCGCGGTTGATGGAGGCGa > 1:1515498/1‑71 (MQ=255) gccgccCAGCTCTTTGGCTTCTATCAGCGCACATTTCTGGCCGTACATAGCCGCGCGGTTGATGGAGGCGa > 1:3227311/1‑71 (MQ=255) gccgccCAGCTCTTTGGCTTCTATCAGCGCACATTTCTGGCCGTACATAGCCGCGCGGTTGATGGAGGCGa > 1:2614285/1‑71 (MQ=255) ccgccCAGCTCTTTGGCTTCTATCAGCGCACATTTCTGGCCGTACATAGCCGCGCGGTTGATGGAGGCGa > 1:3602067/1‑70 (MQ=255) gccCAGCTCTTTGGCTTCTATCAGCGCACATTTCTGGCCg < 1:1728196/40‑1 (MQ=255) ctctTTGGCTTCTATCAGCGCACATTTCTGGCCGTACATAGCCGCGCGGTTGATGGAGGCGATACcgccgc > 1:2569807/1‑71 (MQ=255) tctTTGGCTTCAATCAGCGCACATTTCTGGCCGTACATAGCCGCGCGGTTGATGGAGGc < 1:1226816/59‑1 (MQ=255) ctTTGGCTTCAATCAGCGCACATTTCTGGCCGTACa > 1:1045735/1‑36 (MQ=255) ctTTGGCTTCAATCAGCGCACATTTCTGGCCGTACa > 1:2326506/1‑36 (MQ=255) ctTTGGCTTCAATCAGCGCACATTTCTGGCCGTACa > 1:2564742/1‑36 (MQ=255) ctTTGGCTTCAATCAGCGCACATTTCTGGCCGTACa > 1:4097198/1‑36 (MQ=255) ctTTGGCTTCAATCAGCGCACATTTCTGGCCGTACa > 1:2779521/1‑36 (MQ=255) ctTTGGCTTCAATCAGCGCACATTTCTGGCCGTACa > 1:750859/1‑36 (MQ=255) ctTTGGCTTCAATCAGCGCACATTTCTGGCCGTACATAGCCGCGCGGTTGa > 1:1451089/1‑51 (MQ=255) tttGGCTTCAATCAGCGCACATTTCTGGCCGTACATAGCCGCGCGGtt < 1:2076934/48‑1 (MQ=255) tttGGCTTCAATCAGCGCACATTTCTGGCCGTACATAGCCGCGCGGtt < 1:3467911/48‑1 (MQ=255) tttGGCTTCAATCAGCGCACATTTCTGGCCGTACATAGCCGCGCGGtt < 1:4165686/48‑1 (MQ=255) tttGGCTTCAATCAGCGCACATTTCTGGCCGTACATAGCCGCGCGGtt < 1:1901346/48‑1 (MQ=255) ttGGCTTCAATCAGCGCACATTTCTGGCCGTACATAGCCGCGCGGt > 1:3431651/1‑46 (MQ=255) ttGGCTTCAATCAGCGCACATTTCTGGCCGTACATAGCCGCGCGGt > 1:2426677/1‑46 (MQ=255) tGGCTTCTATCAGCGCACATTTCTGGCCGTACATAGCCGCGCGGTTGATGGAGGCGATACCGCCGCTgccg > 1:68136/1‑71 (MQ=255) tGGCTTCAATCAGCGCACATTTCTGGCCGTACATAGCCGCGCGGTTGATGGAGGCGATACCGCCGCTgcc > 1:2616020/1‑70 (MQ=255) aaTCAGCGCACATTTCTGGCCGTACATAGCCGCGCGGTTGATGGAGGCGATACCGCCGCTGccgccgccg < 1:417444/70‑1 (MQ=255) aaTCAGCGCACATTTCTGGCCGTACATAGCCGCGCGGTTGATGGAGGCGATACCGCCGCTGccgccgccg < 1:2924650/70‑1 (MQ=255) | CACATCACTTTTTTCGGCACACAGCCAACATTTACGCAGGTGCCGCCCAGCTCTTTGGCTTCAATCAGCGCACATTTCTGGCCGTACATAGCCGCGCGGTTGATGGAGGCGATACCGCCGCTGCCGCCGCCG > minE/2599073‑2599204 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |