| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,651,936 | C→T | 37.4% | A57V (GCG→GTG) | glgC → | glucose‑1‑phosphate adenylyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,651,936 | 0 | C | T | 37.4% | 95.2 / 107.0 | 99 | A57V (GCG→GTG) | glgC | glucose‑1‑phosphate adenylyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (37/25); new base T (18/19); total (55/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.04e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01 | |||||||||||
Only 100 of 105 total aligned reads displayed.
AAGGATTTAACCAATAAGCGAGCAAAACCGGCCGTACACTTCGGCGGTAAGTTCCGCATTATCGACTTTGCGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGCGTGATCACCCAGTACCAGTCC > minE/2651866‑2651997 | aaGGATTTAACCAATAAGCGAGCAAAACCGGCCGTACACTTCGGCGGTAAGTTCCGCATTATCGACTTTgc > 1:3557886/1‑71 (MQ=255)aaGGATTTAACCAATAAGCGAGCAAAACCGGCCGTACACTTCGGCGGTAAGTTCCGCATTATCGACTTTgc > 1:4232496/1‑71 (MQ=255)aaGGATTTAACCAATAAGCGAGCAAAACCGGCCGTACACTTCGGCGGTAAGTTCCGCATTATCGACTTTgc > 1:152418/1‑71 (MQ=255)aaGGATATAACCAATAAGCGAGCAAAACCGGCCGTACACTTAGGCGGTAAGTTCCGCATTATCGACTTTgc > 1:542098/1‑71 (MQ=255) aGGATTTAACCAATAAGCGAGCAAAACCGGCCGTACACTTCGGCGGTAAGTTCCGCATTGTCGACTTTgcg > 1:2014908/1‑71 (MQ=255) aGGATTTAACCAATAAGCGAGCAAAACCGGCCGTACACTTCGGCGGTAAGTTCCGCATTATCGACTTTgcg > 1:1909391/1‑71 (MQ=255) gATTTAGCCAATAAGCGAGCAAAACCGGCCGTACACTTCGGCGGTAAGTTCCGCATTATCGACTTTGCGCt > 1:545008/1‑71 (MQ=255) gATTTAACCAATAAGCGAGCAAAACCGGCCGTACACTTCGGCGGTAAGTTCCGCATTATCGACTTTGCGCt > 1:3846376/1‑71 (MQ=255) aTTTAACCAATAAGCGAGCAAAACCGGCCGTACACTTCGGCGGTAAGTTGCGCATTATCGACTTTGTGCTg > 1:1889679/1‑71 (MQ=255) aTTTAACCAATAAGCGAGCAAAACCGGCCGTACACTTCGGCGGTAAGTTCCGCATTATCGACTTTGTGCTg > 1:1975712/1‑71 (MQ=255) aTTTAACCAATAAGCGAGCAAAACCGGCCGTACACTTCGGCGGTAAGTTCCGCATTATCGACTTTGCGCTg < 1:4072046/71‑1 (MQ=255) tAACCAATAAGCGAGCAAAACCGGCCGTACACTTCGGCGGTAAGTTCCGCATTATCGACTTTGCGCTGTc > 1:2055179/1‑70 (MQ=255) aaCCAATAAGCGAGCAAAACCGGCCGTACACTTCGGCGGTAAGTTCCGCATTATCGACTTTGCGCTGTCt < 1:1466415/70‑1 (MQ=255) aCCAATAAGCGAGCAAAACCGGCCGTACACTTCGGCGGTAAGTTCCGCATTATCGACTTTGCGCTGTCTaa < 1:3572290/71‑1 (MQ=255) aCCAATAAGCGAGCAAAACCGGCCGTACACTTCGGCGGTAAGTTCCGCATTATCGACTTTGCGCTGTCTa < 1:1959813/70‑1 (MQ=255) aCCAATAAGCGAGCAAAACCGGCCGTACACTTCGGCGGTAAGTTCCGCATTATCGACTTTGCGCTGTCTa < 1:3833718/70‑1 (MQ=255) ccAATAAGCGAGCAAAACCGGCCGTACACTTCGGCGGTAAGTTCCGCATTATCGACTTTGTGCTGTCTAAc > 1:1168955/1‑71 (MQ=255) tAAGCGAGCAAAACCGGCCGTACACTTCGGCGGTAAGTTCCGCATTATCGACTTTGTGCTGTCTAACTGCa > 1:3130160/1‑71 (MQ=255) aaGCGAGCAAAACCGGCCGTACACTTCGGCGGTAAGTTCCGCATTATCGACTTTGCGCTGTCTaa > 1:3311663/1‑65 (MQ=255) aGCGAGCAAAACCGGCCGTACACTTCGGCGGTAAGTTCCGCATTATCGACTTTGTGCTGTCTAACTGCAt > 1:303797/1‑70 (MQ=255) aGCGAGCAAAACCGGCCGTACACTTCGGCGGTAAGTTCCGCATTATCGACTTTGTGCTGTCTAACTGCAt > 1:859315/1‑70 (MQ=255) aGCGAGCAAAACCGGCCGTACACTTCGGCGGTAAGTTCCGCATTATCGACTTTGTGCTGTCTAACTGCAt > 1:111773/1‑70 (MQ=255) aGCGAGCAAAACCGGCCGTACACTTCGGCGGTAAGTTCCGCATTATCGACTTTGTGCTGTCTAACTGCAt > 1:501775/1‑70 (MQ=255) ccGGCCGTACACTTCGGCGGTAAGTTCCGCATTATCGCCTTTGCGCTGTCTAACTGCATCAACTCCGGGAt > 1:1007479/1‑71 (MQ=255) ccGGCCGTACACTTCGGCGGTAAGTTCCGCATTATCGACTTTGCGCTGTCTAACTGCATCAACTCCGGGAt > 1:1261681/1‑71 (MQ=255) cGGCCGTACACTTCGGCGGTAAGTTCCGCATTATCGACTTTGTGCTGTCTAACTGCATCAACTCCGGGATc > 1:2127378/1‑71 (MQ=255) cGGCCGTACACTTCGGCGGTAAGTTCCGCATTATCGACTTTGTGCTGTCTAACTGCATCAACTCCGGGATc > 1:1213321/1‑71 (MQ=255) cGGCCGTACACTTCGGCGGTAAGTTCCGCATTATCGACTTAGCGCTg < 1:1516904/47‑1 (MQ=255) ggCCGTACACTTCGGCGGTAAGTTCCGCATTATCGACTTTGTGCTGTCTAACTGCATCAACTCCGGGATcc < 1:2469320/71‑1 (MQ=255) ggCCGTACACTTCGGCGGTAAGTTCCGCATTATCGACTTTGTGCTGTCTAACTGCATCAACTCCGGGATcc < 1:2338135/71‑1 (MQ=255) gCCGTACACTTCGGCGGTAAGTTCCGCATTATCGACTTTGTGCTGTCTAACTGCATCAACTCCGGGATCcg > 1:4103458/1‑71 (MQ=255) gCCGTACACTTCGGCGGTAAGTTCCGCATTATCGACTTTGTGCTGTCTAACTGCATCAACTCCGGGATCcg > 1:1224954/1‑71 (MQ=255) gTACACTTCGGCGGTAAGTTCCGCATTATCGACTTTGCGCTGTCTAACTGCATCAACTCCGGGATCcgtcg < 1:1242708/71‑1 (MQ=255) tACACTTCGGCGGTAAGTTCCGCATTATCGACTTTGCGCTGTCTAACTGCATCAACTCCGGGATCcgt < 1:3703639/68‑1 (MQ=255) acacTTCGGCGGTAAGTTCCGCATTATCGACTTTGTGCTGTCTAACTGCATCAACTCCGTGATCcgtcgt < 1:1373832/70‑1 (MQ=255) acacTTCGGCGGTAAGTTCCGCATTATCGACTTTGTGCTGTCTAACTGCATCAACTCCGGGATTcgtcgt < 1:540774/70‑1 (MQ=255) acacTTCGGCGGTAAGTTCCGCATTATCGACTTTGTGCTGTCTAACTGCATCAACTCCGGGATCcgtcgt < 1:2803903/70‑1 (MQ=255) acacTTCGGCGGTAAGTTCCGCATTATCGACTTTGTGCTGTCTAACTGCATCAACTCCGGGATCcgtcgt < 1:865739/70‑1 (MQ=255) acacTTCGGCGGTAAGTTCCGCATTATCGACTTTGTGCTGTCTAACTGCATCAACTCCGGGATCcgtcgt < 1:1981682/70‑1 (MQ=255) acacTTCGGCGGTAAGTTCCGCATTATCGACTTTGTGCTGTCTAACTCCATCAACTCCGGGATCcgtcgt < 1:3189851/70‑1 (MQ=255) cacTTCGGCGGTAGGTTCCGCATTATCGACTTTGCGCTGTCTAACTGCATCAACTCCGGGATCcgtcg > 1:1427373/1‑68 (MQ=255) cacTTCGGCGGTAAGTTCCGCATTATCGACTTTGCGCTGTCTAACTGCATCAACTCCGGGATCcgtcg > 1:2383347/1‑68 (MQ=255) cacTTCGGCGGTAAGTTCCGCATTATCGACTTTGCGCTGTCTAACTGCATCAACTCCGGGATCcgtcg > 1:2082696/1‑68 (MQ=255) cacTTCGGCGGTAAGTTCCGCATTATCGACTTTGCGCTGTCTAACTGCATCAACTCCGGGATCcgtcg > 1:4203130/1‑68 (MQ=255) cacTTCGGCGGTAAGTTCCGCATTATCGACTTTGCGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTAt > 1:1869204/1‑71 (MQ=255) cacTTCGGCGGTAAGTTCCGCATTATCGACTTTGCGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTAt < 1:1942415/71‑1 (MQ=255) cacTTCGGCGGTAAGTTCCGCATTATCGACTTTGCGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTAt < 1:1028355/71‑1 (MQ=255) cacTTCGGCGGTAAGTTCCGCATTATCGACTTTGCGCTGTATAAATGCATCAACTCCGGGAt > 1:3285883/1‑62 (MQ=255) cTTCGGCGGTAAGTTCCGCATTATCGACTTTGTGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATgg < 1:3904353/71‑1 (MQ=255) gcggTAAGTTCCGCATTATCGACTTTGTGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGTCGTg < 1:2608155/71‑1 (MQ=255) gcggTAAGTTCCGCATTATCGACTTTGCGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGCGTg < 1:2211116/71‑1 (MQ=255) cggTAAGTTCCGCATTATCGACTTTGCGCTGTCTAACTGCATCAACTCCggg < 1:2159896/52‑1 (MQ=255) ggTAAGTTCCGCATTATCGACTTTGCGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGCGTGAt > 1:2545779/1‑71 (MQ=255) gTAAGTTCCGCATTATCGACTTTGTGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGCGTg < 1:1337115/68‑1 (MQ=255) gTAAGTTCCGCATTATCGACTTTGTGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGCGTg < 1:101730/68‑1 (MQ=255) gTAAGTTCCGCATTATCGACTTTGTGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGCGTg < 1:3516179/68‑1 (MQ=255) gTAAGTTCCGCATTATCGACTTTGCGCTGTCTAACTGCATCAACTCCGGGAt > 1:1098628/1‑52 (MQ=255) gTAAGTTCCGCATTATCGACTTTGCGCTGTCTAACTGCATCAACTCCGGGAt > 1:1054158/1‑52 (MQ=255) gTAAGTTCCGCATTATCGACTTTGCGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGCGTGa < 1:3344063/69‑1 (MQ=255) gTAAGTTCCGCATTATCGACTTTGCGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGCGTGa < 1:2189392/69‑1 (MQ=255) aaGTTCCGCATTATCGACTTTGTGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGCg > 1:2761467/1‑64 (MQ=255) aaGTTCCGCATTATCGACTTTGTGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGCg > 1:3109977/1‑64 (MQ=255) aaGTTCCGCATTATCGACTTTGCGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGCGTGAt > 1:1845091/1‑68 (MQ=255) aaGTTCCGCATTATCGACTTTGCGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGCGTGATCAc > 1:317250/1‑71 (MQ=255) ttCCGCATTATCGACTTTGTGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGc > 1:2639470/1‑60 (MQ=255) ttCCGCATTATCGACTTTGTGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGCg > 1:1332126/1‑61 (MQ=255) ttCCGCATTATCGACTTTGTGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGCg > 1:3948710/1‑61 (MQ=255) ttCCGCATTATCGACTTTGTGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGCg > 1:3573887/1‑61 (MQ=255) ccGCATTATCGACTTTGCGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGCGTGATCACCCAg > 1:1014864/1‑70 (MQ=255) ccGCATTATCGACTTTGCGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGCGTGATCACCCAg > 1:3590597/1‑70 (MQ=255) ccGCATTATCGACTTTGCGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGCGTGATCACCCAg > 1:429622/1‑70 (MQ=255) ccGCATTATCGACTTTGCGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGCGTGATCACCCAg > 1:713725/1‑70 (MQ=255) ccGCATTATCGACTTTGCGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGCGTGATCACCCAg > 1:1031448/1‑70 (MQ=255) ccGCATTATCGACTTTGCGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGCGTGATCACCCAg > 1:2755070/1‑70 (MQ=255) ccGCATTATCGACTTTGCGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGCGTGATCACCCAg < 1:1810317/70‑1 (MQ=255) ccGCATTATCGACTTTGCGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGCGTGATCACCCAg < 1:3553927/70‑1 (MQ=255) cGCATTATCGACTTTGCGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGCGTGATCACCCAGt < 1:2833250/70‑1 (MQ=255) cGCATTATCGACTTTGCGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGCGTGATCACCCAGt < 1:4217314/70‑1 (MQ=255) cGCATTATCGACTTTGCGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGCGTGATCACCCAGt < 1:1815388/70‑1 (MQ=255) gCATTATCGACTTTGCGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGCGTGATCACCCAGTAc < 1:1092403/71‑1 (MQ=255) cATTATCGACTTTGTGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGCGTGATCACCCAGTAcc < 1:2034800/71‑1 (MQ=255) cATTATCGACTTTGCGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGCGTGATCACCCAGtgc > 1:3897425/1‑68 (MQ=255) ttATCGACTTTGCTCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGCGTGATCACCCAGTACCAg < 1:156626/71‑1 (MQ=255) tATCGACTTTGTGCTGTCTAACTGCATCAACTCCGGGa < 1:868546/38‑1 (MQ=255) tATCGACTTTGTGCTGTCTAACTGCATCAACTCCGGGa < 1:2027834/38‑1 (MQ=255) tATCGACTTTGCGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTa > 1:4181041/1‑47 (MQ=255) tATCGACTTTGCGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTa > 1:665668/1‑47 (MQ=255) tATCGACTTTGCGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTa > 1:4151220/1‑47 (MQ=255) tATCGACTTTGCGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTa > 1:2860371/1‑47 (MQ=255) tATCGACTTTGCGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTa > 1:3550232/1‑47 (MQ=255) aTCGACTTTGCGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGCGTGATCACCCAGTACCAGTc > 1:1785577/1‑71 (MQ=255) aTCGACTTTGCGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGCGTGATCACCCAGTACCAGTc > 1:1780425/1‑71 (MQ=255) tCGACTTTGTGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGCGTGATCACCCAGTACCAGTcc < 1:2760881/71‑1 (MQ=255) cGACTTTGCGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGCGt > 1:3822990/1‑51 (MQ=255) cGACTTTGCGCTGTCTAACTGCATCAACTACGGGATCCGTCGTATGGGCGTGATCACCCAGTACCa > 1:189449/1‑66 (MQ=255) gACTTTGCGCTGTCTAACTGCATCAACTCCGGGATc > 1:3527142/1‑36 (MQ=255) gACTTTGCGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGCGTGAt > 1:1863667/1‑53 (MQ=255) tttGCGCTGTCTAACTGCATCAACTCCGGGATCcgtcg < 1:1803006/38‑1 (MQ=255) tttGCGCTGTCTAACTGCATCAACTCCGGGATCcgtcg < 1:1777828/38‑1 (MQ=255) tttGCGCTGTCTAACTGCATCAACTCCGGGATCcgtcg < 1:1220124/38‑1 (MQ=255) | AAGGATTTAACCAATAAGCGAGCAAAACCGGCCGTACACTTCGGCGGTAAGTTCCGCATTATCGACTTTGCGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGCGTGATCACCCAGTACCAGTCC > minE/2651866‑2651997 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |